Essentialism: The Disciplined Pursuit of Less

he utilizes three steps in discovering and embracing what is most important: explore, eliminate, and execute. intertwined in these steps are practical stories of corporate executives who discovered their niche and priorities by following these principles. the author gives practical counsel such as getting plenty of sleep, taking time to play, gathering the courage to eliminate things that are not important even if it offends others, and even getting into a flow or routine of doing a few things well while cutting out the non-essential priorities that others place in our path

Men of Valor: A Study of Following Christ\u27s Example of Discipleship with Five Key Men in the Local Church

Problem The Tampa First Seventh-day Adventist Church is a diverse congregation with over 40 countries represented in its membership of nearly 600. There had been a steady increase of attendance and membership through transfers and conversion for several years. One of the biggest challenges that we faced at Tampa First was the discipleship of men. I have perceived in my 23 years of ministry that when the father is growing spiritually, the family unit had a greater tendency to grow with him, and the children were much more likely to stay connected to God and the church. It was imperative for me to find a way to break through the barriers of spiritual stagnation with the men at Tampa First. This project has led the way to spiritual growth and an intentional disciple-making process for these men in my congregation. Task The task of this project was to develop and implement a process of discipleship for a small group the men of the Tampa First Church where I poured myself into five key male leaders over a twelve-month period and empowered them to be disciple-makers for other men in our local congregation and in their spheres of influence. Results After the twelve-month period of discipleship, three of the five key leaders remained active in the group while others moved on for various reasons. I created a spiritual growth measuring tool I called a Life Thermometer consisting of ten questions that was given to the participants at the end of the twelve months. The results of all five participants from the Life Thermometer showed overall increases ranging from 15% up to a 48% increase with an average growth among the five men of 28%. Conclusion There was spiritual growth among all the participants and in me as the group leader over the twelve-month period. We all grew as leaders, men of God, priests in our homes, and witnesses in our spheres of influence. The principles fleshed out in this study will have a significant impact in men’s discipleship at the Tampa First Church as well as possibly impacting other churches in our region and local conference

Differential recognition of the Multiple Banded Antigen isoforms across Ureaplasma parvum and Ureaplasma urealyticum species by monoclonal antibodies

Two separate species of Ureaplasma have been identified that infect humans: Ureaplasma parvum and Ureaplasma urealyticum. Most notably, these bacteria lack a cell wall and are the leading infectious organism associated with infection-related induction of preterm birth. Fourteen separate representative prototype bacterial strains, called serovars, are largely differentiated by the sequence of repeating units in the C-terminus of the major surface protein: multiple-banded antigen (MBA). Monoclonal antibodies that recognise single or small groups of serovars have been previously reported, but these reagents remain sequestered in individual research laboratories. Here we characterise a panel of commercially available monoclonal antibodies raised against the MBA and describe the first monoclonal antibody that cross-reacts by immunoblot with all serovars of U. parvum and U. urealyticum species. We also describe a recombinant MBA expressed by Escherichia coli which facilitated further characterisation by immunoblot and demonstrate immunohistochemistry of paraffin-embedded antigens. Immunoblot reactivity was validated against well characterised previously published monoclonal antibodies and individual commercial antibodies were found to recognise all U. parvum strains, only serovars 3 and 14 or only serovars 1 and 6, or all strains belonging to U. parvum and U. urealyticum. MBA mass was highly variable between strains, consistent with variation in the number of C-terminal repeats between strains. Antibody characterisation will enable future investigations to correlate severity of pathogenicity to MBA isoform number or mass, in addition to development of antibody-based diagnostics that will detect infection by all Ureaplasma species or alternately be able to differentiate between U. parvum, U. urealyticum or mixed infections