Genomic characterization, high-density mapping and anchoring of DArT markers to the reference genome of Eucalyptus

Genetic linkage maps have been essential tools to examine the inheritance of qualitative and quantitative traits, to carry out comparative mapping and to provide markers for molecular breeding applications. Linkage maps for species of Eucalyptus have been reported for several pedigrees using different molecular marker technologies [1]. However improved marker density, throughput and transferability across species are necessary to increase resolution of current maps for a variety of genomic applications. We report the development of a high density linkage map for Eucalyptus based on microsatellites and DArT (Diversity Arrays Technology) markers generated by a standardized genotyping microarray [2]. DNA probes that constitute the DArT microarray were sequenced and positioned on the reference Eucalyptus genome providing information about their sequence content, their distribution relative to annotated genes as well as the relationship between physical and recombination distance in the Eucalyptus genome. [Poster Presentation

High realized accuracies of genomic selection for volume growth and wood density in Eucalyptus breeding populations with contrasting effective sizes.

W235: Forest Trees

Computação da Seleção Genômica Ampla (GWS).

Métodos para GWS; Teoria dos métodos de regressão; Computação do método Random (Ridge) Regression BLUP (RR-BLUP/GWS); Fenótipos corrigidos; Frequências alélicas, variância dos marcadores e herdabilidade; Marcadores codominantes (SNP) ? Modelo genotípico; Marcadores dominantes (DArT) - Modelo genotípico; Marcadores codominantes (SNP) ? Modelo gamético ou alélico; Número de marcadores com efeitos significativos; Populações de estimação, validação e seleção; População de validação e Jacknife; Correlação e regressão entre valores genéticos preditos e fenótipos na população de validação; Análise de associação na GWAS; Software Selegen Genômica: Random (Ridge) Regression BLUP: RR-BLUP/GWS; Exemplo aplicado ao melhoramento do eucalipto.bitstream/item/31426/1/Doc210.pd