7 research outputs found
Nano‐ and Microscale Confinements in DNA‐Scaffolded Enzyme Cascade Reactions
Artificial reconstruction of naturally evolved principles, such as compartmentalization and cascading of multienzyme complexes, offers enormous potential for the development of biocatalytic materials and processes. Due to their unique addressability at the nanoscale, DNA origami nanostructures (DON) have proven to be an exceptionally powerful tool for studying the fundamental processes in biocatalytic cascades. To systematically investigate the diffusion-reaction network of (co)substrate transfer in enzyme cascades, a model system of stereoselective ketoreductase (KRED) with cofactor regenerating enzyme is assembled in different spatial arrangements on DNA nanostructures and is located in the sphere of microbeads (MB) as a spatially confining nano- and microenvironment, respectively. The results, obtained through the use of highly sensitive analytical methods, Western blot-based quantification of the enzymes, and mass spectrometric (MS) product detection, along with theoretical modeling, provide strong evidence for the presence of two interacting compartments, the diffusion layers around the microbead and the DNA scaffold, which influence the catalytic efficiency of the cascade. It is shown that the microscale compartment exerts a strong influence on the productivity of the cascade, whereas the nanoscale arrangement of enzymes has no influence but can be modulated by the insertion of a diffusion barrier
Self-immobilizing Biocatalysts for fluidic Reaction Cascades
The industrial implementation of whole-cells and enzymes in flow biocatalysis microreactors is essential for the
emergence of a biobased circular economy. Major challenges concern the efficient immobilization of delicate enzymes
inside miniaturized reactors without compromising their catalytic activity. We describe the design and application of four
different immobilization techniques including self-immobilizing whole-cells and purified enzymes on magnetic
microbeads, as well as reactor modules manufactured by 3D printing of bioinks containing thermostable enzymes. To
increase the volumetric activity of our microreactors we furthermore developed and applied self-assembling all-enzyme
hydrogels with cofactor-regenerating capabilities. The resulting reactor formats have excellent operational stability times
of > 14 days and maximum space-time yields of > 450 g product/L-1day-1 paving the way for mild and effective
immobilization techniques of biocatalysts in microfluidic systems
Identification of a small-molecule ligand of the epigenetic reader protein Spindlin1 via a versatile screening platform
Epigenetic modifications of histone tails play an essential role in the regulation of eukaryotic transcription. Writer and eraser enzymes establish and maintain the epigenetic code by creating or removing posttranslational marks. Specific binding proteins, called readers, recognize the modifications and mediate epigenetic signalling. Here, we present a versatile assay platform for the investigation of the interaction between methyl lysine readers and their ligands. This can be utilized for the screening of smallmolecule inhibitors of such protein–protein interactions and the detailed characterization of the inhibition. Our platform is constructed in a modular way consisting of orthogonal in vitro binding assays for ligand screening and verification of initial hits and biophysical, label-free techniques for further kinetic characterization of confirmed ligands. A stability assay for the investigation of target engagement in a cellular context complements the platform. We applied the complete evaluation chain to the Tudor domain containing protein Spindlin1 and established the in vitro test systems for the double Tudor domain of the histone demethylase JMJD2C. We finally conducted an exploratory screen for inhibitors of the interaction between Spindlin1 and H3K4me3 and identified A366 as the first nanomolar small-molecule ligand of a Tudor domain containing methyl lysine reader