Transport study of adsorptively HI 6 dichloride monohydrate-loaded nanoparticles on an <i>in vitro</i> BBB model.

<p>Transport study of adsorptively HI 6 dichloride monohydrate-loaded nanoparticles on an <i>in vitro</i> BBB model.</p

Long time measurement of transendothelial electrical resistance (TER) after nanoparticle addition.

<p>pBCEC were seeded on collagen IV-coated Transwell inserts and incubated with the free drug or 0.26 mg nanoparticles per cm² growth area of the ApoE-modified (NP-ApoE) as well as the PEGylated (NP-PEG) nanoparticulate formulations, which were loaded with 1000 µM of HI 6 dichloride monohydrate (HI 6-DCL) at 37°C by adding the nanoparticles into the upper/apical compartment of the Transwell system. The TER was measured automatically every hour by impedance measurement. A magnification of the area of interest is highlighted in the red quadrangle. As control the measurement of the TER of a Transwell insert without cells is shown.</p

Transport study of adsorptively obidoxime-loaded nanoparticles on an <i>in vitro</i> BBB model.

<p>Transport study of adsorptively obidoxime-loaded nanoparticles on an <i>in vitro</i> BBB model.</p

Transport study of adsorptively HI 6 dimethanesulfonate-loaded nanoparticles on an <i>in vitro</i> BBB model.

<p>*Mann-Whitney U Test: significant difference between NP-ApoE and free HI 6-DMS (P<0.05).</p

Measurement of transendothelial electrical resistance (TER) and the capacitance (C_cl).

<p>pBCEC were seeded on collagen IV-coated Transwell inserts, which were placed in the cellZscope. The transendothelial electrical resistance (TER) and the capacitance (C_cl) of the pBCEC were measured automatically every hour by impedance measurement. As control the measurement of the transendothelial electrical resistance (TER) of a Transwell insert without cells is shown.</p