MICROBIAL STRAINS AND METHODS OF MAKING AND USING

Microbial strains are provided, as are methods of making and using such microbial strains. Methane is an increasingly important global energy Source, both as the primary component of natural gas and as a biologically produced fuel. Currently, Sweden uses 100% renewable methane (biogas) in place of compressed natural gas (CNG) for transportation fuel, and several European countries are implementing policies to increase their pro portion of biogas transportation fuel. In addition, methane is a valuable chemical for conversion into other higher-order carbon compounds (e.g., methanol, formate, alkanes, ter penes). More than 50% of the world\u27s methane is produced biologically by anaerobic archaea called methanogens, but one significant limitation with industrial scale biological methane production from any type of feedstock is that the rate is relatively slow. Methanosarcina acetivorans is one of a unique group of organisms capable of producing methane from both acetate and methylated compounds, which are common anaerobic fermentation end products. A genetically engineered M. acetivorans strain is described herein that exhibits an increased rate of methane production on methanol and on acetate. Such genetically engineered microorganisms will be an essential tool in the ability to use methanogens as an inexpensive source of methane fuel derived from renewable biomass

MICROBAL STRANS AND METHODS OF MAKING AND USING

Microbial strains are provided, as are methods of making and using Such microbial strains

PRODUCTION OF ISOPRENE BY METHANE - PRODUCING ARCHAEA

Plasmid vectors and use of plasmid vectors in methods for producing methane and isoprene using Archaea are disclosed . Particularly, plasmid vectors that express isoprene synthase ( ispS ) are prepared and inserted into methanogens, such as Methanosarcina acetivorans, to allow for co - production of methane and isoprene. In one embodiment , the methods of the present disclosure can be used for wastewater management

PRODUCTION OF ISOPRENE BY METHANE - PRODUCING ARCHAEA

Plasmid vectors and use of plasmid vectors in methods for producing methane and isoprene using Archaea are disclosed . Particularly, plasmid vectors that express isoprene synthase ( ispS ) are prepared and inserted into methanogens, such as Methanosarcina acetivorans, to allow for co - production of methane and isoprene. In one embodiment , the methods of the present disclosure can be used for wastewater management