Complete Genome Sequence of Pelosinus fermentans JBW45, a Member of a Remarkably Competitive Group of Negativicutes in the Firmicutes Phylum.

The genome of Pelosinus fermentans JBW45, isolated from a chromium-contaminated site in Hanford, Washington, USA, has been completed with PacBio sequencing. Nine copies of the rRNA gene operon and multiple transposase genes with identical sequences resulted in breaks in the original draft genome and may suggest genomic instability of JBW45

The Black Hole Mass of NGC 4151. II. Stellar Dynamical Measurement from Near-Infrared Integral Field Spectroscopy

We present a revised measurement of the mass of the central black hole (Mbh) in the Seyfert 1 galaxy NGC 4151. The new stellar dynamical mass measurement is derived by applying an axisymmetric orbit-superposition code to near-infrared integral field data obtained using adaptive optics with the Gemini NIFS spectrograph. When our models attempt to fit both the NIFS kinematics and additional low spatial resolution kinematics, our results depend sensitively on how chi-squared is computed--probably a consequence of complex bar kinematics that manifest immediately outside the nuclear region. The most robust results are obtained when only the high spatial resolution kinematic constraints in the nuclear region are included in the fit. Our best estimates for the BH mass and H-band mass-to-light ratio are Mbh~(3.76+/-1.15)E7 Msun (1-sigma error) and M/L(H-band)~0.34+/-0.03 Msun/Lsun (3-sigma error), respectively (the quoted errors reflect the model uncertainties). Our BH mass measurement is consistent with estimates from both reverberation mapping (3.57[+0.45/-0.37]E7 Msun) and gas kinematics (3.0[+0.75/-2.2]E7 Msun; 1-sigma errors), and our best-fit mass-to-light ratio is consistent with the photometric estimate of M/L(H-band)=0.4+/-0.2 Msun/Lsun. The NIFS kinematics give a central bulge velocity dispersion sigma_c=116+/-3 km/s, bringing this object slightly closer to the M-sigma relation for quiescent galaxies. Although NGC 4151 is one of only a few Seyfert 1 galaxies in which it is possible to obtain a direct dynamical BH mass measurement--and thus, an independent calibration of the reverberation mapping mass scale--the complex bar kinematics makes it less than ideally suited for this purpose.Comment: 21 pages, 15 figures. Accepted for publication in Ap

Psychological Symptoms Among 2032 Youth Living with HIV: A Multisite Study

This study determined the prevalence and patterns of psychological symptoms in adolescents and young adults living with HIV (YLWH) in medical care and relationships between psychological symptoms, route and duration of infection, and antiretroviral treatment (ART). A clinic-based sample of 2032 YLWH (mean age 20.3 years), recruited from 20 adolescent medicine HIV clinics, completed a cross-sectional survey of health behaviors and psychological symptoms using the Brief Symptom Inventory (BSI). Overall, 17.5% of youth reported psychological symptoms greater than the normative threshold on the Global Severity Index. A wide variety of symptoms were reported. The prevalence of clinical symptoms was significantly greater in youth with behaviorally acquired HIV compared to those with perinatally acquired infection (20.6% vs. 10.8%, OR=2.06 in Multiple Logistic Regression (MLR)), and in those not taking ART that had been prescribed (29. 2% vs. 18.8%, OR=1.68 in MLR). Knowing one's HIV status for more than one year and disclosure of HIV status were not associated with fewer symptoms. A large proportion of YLWH have psychological symptoms and the prevalence is greatest among those with behaviorally acquired infection. The high rate of psychological symptoms for youth not taking ART that is prescribed is a cause for concern. Symptoms do not appear to be a transient reaction to diagnosis of HIV.Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/140176/1/apc.2014.0113.pd

Conducting a Journal Assessment Project Using Microsoft Access to Obtain Faculty Input and Promote the Creation of a Cost-Effective Journal Collection

Himmelfarb Health Sciences Library created a database using Microsoft Access to execute a value assessment project of its online and print journal collection. This poster presentation summarizes the process of creating a cost-effective journal collection

All Hands on Deck: A Team Approach to System Migration

Purpose: To determine if a team approach to a complex integrated library system (ILS) migration would result in a timely and successful outcome. Brief Description: Changing needs and an aging integrated library system server platform prompted Himmelfarb Health Sciences Library to migrate to a new ILS. After considering several options, Himmelfarb decided to join with the George Washington University\u27s Jacob Burns Law Library in their move to a hosted instance of Innovative Interface\u27s Sierra ILS and Encore OPAC. Though some separation of data and functionality could be achieved, there were several functions that would be shared between the two libraries\u27 systems. A successful transition would depend upon both libraries\u27: detailed knowledge of the system data determining essential functionality to preserve willingness to change and accommodate Because the Law Library\u27s Sierra migration was delayed until mid-December 2012 and Himmelfarb\u27s pre-paid server maintenance was ending in March 2013, the window to accomplish migration was a short 12 weeks, prompting the decision to team-manage the migration. Conclusion: Teams and organizational tools, including weekly meetings to discuss problems, ensured the timely completion of key assignments that were critical to meeting the Go Live date goal. The team approach allowed the work and decision-making to be distributed among a large percentage of the staff, creating a broader sense of ownership. Distribution of tasks allowed a high level of attention to detail necessary for an optimal outcome

Increasing interest in social studies: Social perspective taking and self-efficacy in stimulating simulations

This study examined the potential of simulations to bolster interest in middle school social studies classrooms. Using a pre-post design, we examined 305 middle school students (49% female) who participated in the web-based GlobalEd simulation. Unlike the motivation declines middle school students usually experience, participants in this simulation became more interested in social studies. We investigated four hypotheses as to why these increases may have occurred. We found no support for the possibility that, (a) students’ interest in a particular issue area or (b) their increased valuing of the subject matter, were related to their increased interest. However, results suggested that, (c) the challenging nature of the activity may have bolstered their interest in social studies and (d) students’ increased propensity to engage in social perspective taking. The discussion explores future research directions and whether implications for classroom teachers are warranted given the correlational nature of the research

Reassortant Pandemic (H1N1) 2009 Virus in Pigs, United Kingdom

Surveillance for influenza virus in pigs in the United Kingdom during spring 2010 detected a novel reassortant influenza virus. This virus had genes encoding internal proteins from pandemic (H1N1) 2009 virus and hemagglutinin and neuraminidase genes from swine influenza virus (H1N2). Our results demonstrate processes contributing to influenza virus heterogeneity

Chronic late-gestation hypoglycemia upregulates hepatic PEPCK associated with increased PGC1alpha mRNA and phosphorylated CREB in fetal sheep.

Hepatic glucose production is normally activated at birth but has been observed in response to experimental hypoglycemia in fetal sheep. The cellular basis for this process remains unknown. We determined the impact of 2 wk of fetal hypoglycemia during late gestation on enzymes responsible for hepatic gluconeogenesis, focusing on the insulin-signaling pathway, transcription factors, and coactivators that regulate gluconeogenesis. Hepatic phosphoenolpyruvate carboxykinase and glucose-6-phosphatase mRNA increased 12-fold and 7-fold, respectively, following chronic hypoglycemia with no change in hepatic glycogen. Chronic hypoglycemia decreased fetal plasma insulin with no change in glucagon but increased plasma cortisol 3.5-fold. Peroxisome proliferator-activated receptor-gamma coactivator-1alpha mRNA and phosphorylation of cAMP response element binding protein at Ser(133) were both increased, with no change in Akt, forkhead transcription factor FoxO1, hepatocyte nuclear factor-4alpha, or CCAAT enhancer binding protein-beta. These results demonstrate that chronic fetal hypoglycemia triggers signals that can activate gluconeogenesis in the fetal liver