Appendix A. A table of microhabitat use by morphotypes of Herichthys minckleyi.

A table of microhabitat use by morphotypes of Herichthys minckleyi

Appendix B. A table of feeding behaviors used by morphotypes of Herichthys minckleyi.

A table of feeding behaviors used by morphotypes of Herichthys minckleyi

Scatter plot of the relative concentrations of peaks 16–18 against pronotum width of male Japanese rhinoceros beetles (<i>Trypoxylus dichotomus)</i>.

Peaks 16 (A) and 17 (B) are both negatively associated with body size and peak 18 (C) is positively associated with body size.</p

List of likely cuticular hydrocarbons (CHCs) present on the elytra of male and female Japanese rhinoceros beetles (<i>Trypoxylus dichotomus)</i>.

List of likely cuticular hydrocarbons (CHCs) present on the elytra of male and female Japanese rhinoceros beetles (Trypoxylus dichotomus).</p

Fig 4 -

Scatter plots of PC1Males (A) and PC2Males (B) against condition. Condition was estimated by taking the residuals of the regression relationship between mass and pronotum width. There is no relationship between PC1Males or PC2Males and condition.</p

Male and female rhinoceros beetles (<i>Trypxoylus dichotomus</i>) vary in cuticular hydrocarbon profile.

(A) Comparison of truncated gas chromatogram spectra obtained from samples made by washing the elytra of a male beetle (depicted in black) and female beetle (depicted in red) with hexanes. Peaks 11,12, and 15 are present across 18 female samples and were not found in any of the 49 males while peaks 16 and 17 are present in males and not females. (B) Principal component 1 (PC1All-beetles) vs. principal component 2 (PC2All-beetles) of the relative abundance of cuticular hydrocarbons found in both male (black solid markers) and female (red open markers) beetles.</p

Individual beetle data.

Measurement and CHC proportion data from the individual beetles used in all of the analyses in this study. (XLSX)</p

Different sized male Japanese rhinoceros beetles (<i>Trypxoylus dichotomus</i>) vary in cuticular hydrocarbon profile.

(A) Comparison of truncated gas chromatogram spectra obtained from samples made by washing the elytron of a relatively large male beetle (black line; pronotum width 22.91mm) and smaller male beetle (red line; pronotum width 20.80mm) with hexanes. Males produce the same peaks across different body sizes, but vary in relative abundance. (B) Principal component 1 (PC1Males) of the relative abundance of cuticular hydrocarbons found in male beetles vs pronotum width. High scores on PC1 are associated with larger body size.</p

Mass spectra examples.

Example mass spectra for gas chromatogram Peaks 1–37 from Trypoxylus dichotomus. Spectra obtained from samples collected by washing elytra with hexanes. (PDF)</p

Principal component (PC) information for the two PC analyses of cuticular hydrocarbon composition.

Principal component (PC) information for the two PC analyses of cuticular hydrocarbon composition.</p