A new simple method with high precision for determining the toxicity of antifouling paints on brine shrimp larvae (<i>Artemia</i>): First results

The use of antifouling paints is the only truly effective method for the protection of underwater structures from the development of fouling organisms. In the present study, the surface to volume concept constitutes the basis for the development of a new and improved method for determining the toxicity of antifouling paints on marine organisms. Particular emphasis is placed on the attainment of a standardized uniformity of coated surfaces. Failure to control the thickness of the coat of paint in previous studies of this type, has led to inaccurate evaluation of the relative toxicity of samples. Herein, an attempt is made to solve this problem using a simple technique which gives completely uniform and smooth surfaces. The effectiveness of this technique is assessed through two series of experiments using two different types of test containers: 50 ml modified syringes and 7 ml multiwells. The results of the toxicity experiments follow a normal distribution around the average value which allows to consider these values as reliable for comparison of the level of toxic effect detected with the two types of test containers. The mean lethal concentration L(S/V)50 in the test series conducted in the multiwells (20.38 mm2 ml-1) does not differ significantly from that obtained in the test series using modified syringes (20.065 mm2 ml-1). It can thus be concluded from this preliminary study that the new method and the two different ways of exposing the test organisms to the antifouling paints and their leachates gave reliable and replicable results

A new simple method with high precision for determining the toxicity of antifouling paints on brine shrimp larvae (Artemia): First results

The use of antifouling paints is the only truly effective method for the protection of underwater structures from the development of fouling organisms. In the present study, the surface to volume concept constitutes the basis for the development of a new and improved method for determining the toxicity of antifouling paints on marine organisms. Particular emphasis is placed on the attainment of a standardized uniformity of coated surfaces. Failure to control the thickness of the coat of paint in previous studies of this type, has led to inaccurate evaluation of the relative toxicity of samples. Herein, an attempt is made to solve this problem using a simple technique which gives completely uniform and smooth surfaces. The effectiveness of this technique is assessed through two series of experiments using two different types of test containers: 50 ml modified syringes and 7 ml multiwells. The results of the toxicity experiments follow a normal distribution around the average value which allows to consider these values as reliable for comparison of the level of toxic effect detected with the two types of test containers. The mean lethal concentration L(S/V)50 in the test series conducted in the multiwells (20.38 mm2 ml-1) does not differ significantly from that obtained in the test series using modified syringes (20.065 mm2 ml-1). It can thus be concluded from this preliminary study that the new method and the two different ways of exposing the test organisms to the antifouling paints and their leachates gave reliable and replicable results. © 2006 Elsevier Ltd. All rights reserved

A new simple method with high precision for determining the toxicity of antifouling paints on brine shrimp larvae (Artemia): First results

The use of antifouling paints is the only truly effective method for the protection of underwater structures from the development of fouling organisms. In the present study, the surface to volume concept constitutes the basis for the development of a new and improved method for determining the toxicity of antifouling paints on marine organisms. Particular emphasis is placed on the attainment of a standardized uniformity of coated surfaces. Failure to control the thickness of the coat of paint in previous studies of this type, has led to inaccurate evaluation of the relative toxicity of samples. Herein, an attempt is made to solve this problem using a simple technique which gives completely uniform and smooth surfaces. The effectiveness of this technique is assessed through two series of experiments using two different types of test containers: 50 ml modified syringes and 7 ml multiwells. The results of the toxicity experiments follow a normal distribution around the average value which allows to consider these values as reliable for comparison of the level of toxic effect detected with the two types of test containers. The mean lethal concentration L(S/V)50 in the test series conducted in the multiwells (20.38 mm2 ml-1) does not differ significantly from that obtained in the test series using modified syringes (20.065 mm2 ml-1). It can thus be concluded from this preliminary study that the new method and the two different ways of exposing the test organisms to the antifouling paints and their leachates gave reliable and replicable results. © 2006 Elsevier Ltd. All rights reserved

ALDH activity of artemia as a tool for the investigation of the toxicity of antifouling paints

Leaching of toxic substances from the matrix of antifouling paints affects not only the fouling organisms but also "non-target" biota. Artemia nauplii have been found to be suitable test organisms for measurement of acute toxicity effects of certain antifouling paints. The present study addresses the impact of a self polishing copper antifouling paint on the activity of the brine shrimp aldehyde dehydrogenase (ALDH). Total ALDH activities from nauplii exposed to several sublethal concentrations ranging from (S/V)50 to 1/4(S/V)50 were determined. Decreased enzymatic activities were observed in all antifouling paint concentrations tested, ranging from 2.4% inhibition at 1/4(S/V)50 to 53.9% at (S/V)50. The results of this preliminary investigation suggest that ALDH activity of Artemia nauplii could be used as a biomarker for the evaluation of toxic activity of antifouling paint on non target organisms. © 2013 by Nova Science Publishers, Inc. All rights reserved