Alkaloid isomerization induced by spray drying of Uncaria tomentosa bark extracts

Cat's claw (Uncaria tomentosa (Willd) DC) is an alkaloid rich-liana from the Peruvian Amazon rainforest. Regarding several oxindole alkaloids from cat's claw bark, the isomerization induced by pH, temperature and solvent polarity is reported in the literature. Some industrial drying processes, such as spray drying, can provide therefore favorable conditions of heating and moisture for isomerization, and it is expected to have effects on the alkaloid composition during technological processing. In this work we focused on the effect of spray drying on the isomerization of alkaloids of dried extracts obtained from Uncaria tomentosa bark. High isomerization levels were observed in all dried extracts. A loss in mitraphylline (15%) and pteropodine (12%) content is related to an equivalent content increase of isomitraphylline and isopteropodine, respectively. Since different biological and pharmacological activities had been ascribed to these alkaloids, the influence of the thermal effect inherent to the spray drying technique and excipient type should be worth of special attention.Colegio de Farmacéuticos de la Provincia de Buenos Aire

Determination of galangin in commercial extracts of Alpinia officinarum by RP-HPLC-DAD

Alpinia officinarum has been used by long time in the traditional medicine and several commercial extracts containing it are available on the market. In this paper a reversed phase high performance liquid chromatography-diode array detection (RP-HPLC-DAD) method for Alpinia officinarum preparations was proposed using galangin as a marker. The quantification was undertaken by a combination of RP-HPLC-DAD. The LC system employed a Gemini Phenomenex RP-18 column with phosphoric acid, tetrahydrofuran and acetonitrile as mobile phase and detection at 267 nm. The method validation was throughout satisfactory. The determination coefficient was r2 = 0.998 with LOD 0.48 µg mL-1 and LOQ 1.59 µg mL-1 The results of precision (RSD < 2.82) and accuracy (99.9 %, 100.4 %, 102.2 %) with . RSD less than 2.7 %, were satisfactory. The analytical method was applied to commercial extracts, and was suitable to provide qualitative and quantitative information for the quality control to commercial preparations.Colegio de Farmacéuticos de la Provincia de Buenos Aire