Combined treatment of throphic ulcer of the heel using vacuum therapy with direct gene therapy: Case report

In this article we describe a clinical case of successful treatment of neurotrophic ulcers of heel region using classical surgical techniques such as debridement and autodermoplastics combined with vacuum therapy and injection of double cassette expression plasmid encoding cDNAs of vascular endothelial growth factor and fibroblast growth factor 2. The outcome of therapy opens new horizons in the treatment of such diseases in short period of time. © Human stem cells institute, 2013

Combined therapy for contractures of the hand joints

Aim to study the effectiveness of the combined treatment of contractures of proximal interphalangeal joint. Material and methods. The study included 30 patients with a diagnosis of post-traumatic mixed contracture of the proximal interphalangeal joint. The patients were divided into 3 groups. In the first group, the patients were administered a combined treatment, including the arthrolysis of the joint, the imposition of a dynamic distraction apparatus, the injection of hyaluronic acid preparations and platelet-rich plasma. The second group of patients underwent the arthrolysis of the joint and the application of the apparatus. The third group underwent only arthrolysis. Results. The results of the study indicated the effectiveness of all three methods of treatment. However, in the first group where the combined treatment was used, the registered improvement in the angles of joint function and a decrease in pain were significant, which ultimately led to better functional indices of the hand and a shorter treatment period

Use of stromal vascular fraction of adipose tissue for treatment of femur pseudoarthtosis: Case report

The article presents a case report of successful treatment of pseudarthrosis of the femur with the use of autologous cells of the stromal vascular fraction (SVF) derived from adipose tissue. Autologous SVF cells mixed with fibrin glue were transplanted into bone defect by injection under the control of electro-optical converter. Two months after injection, the patient reported the disappearance of pain. After 4 months we observed the recovery of function of the knee, X-ray shows signs of the fracture healing through the formation of bone callus. © Human stem cells institute, 2013

Correction of the face soft tissue defect using autologous fat tissue enriched by cells of stromal-vascular fraction

The article presents a clinical case of successful surgical treatment of a patient with idiopathic progressive hemifacial atrophy. We have used lipofilling with autologous fat tissue enriched by stromal-vascular fraction cells. Observation of the patient within two years suggests that this method of treatment is safe and highly effective method of face soft tissue defect substitution of such pathology

Stimulation of rat's sciatic nerve post-traumatic regeneration using plasmids expressing vascular endothelial growth factor and basic fibroblast growth factor

The development of effective treatments for patients with peripheral nerve injury is an urgent task of biomedicine. «Gold» standard in restoring the integrity of nerve conduits is auto-nerve transplantation in which a peripheral nerve defect is corrected with autologous nerve graft. Here we propose a method for stimulating revascularization and regeneration of auto-nerve graft by a local injection of plasmid pBud-VEGFFGF2, expressing vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (FGF2). It is shown that direct injection of plasmid pBud-VEGF-FGF2 in the proximal and distal segments of nerve, as well as in the auto-nerve graft, stimulates the regeneration of the rat's sciatic nerve and restores motor activity

The first clinical experience of direct gene therapy using VEGF and bFGF in treatment patients with critical lower limb ischemia

In this paper we present the clinical observation of successful treatment of distal form of peripheral artery disease of the lower extremity with symptoms of critical ischemia in a 60 years old patient. Intramuscular injection of dual expression plasmid, encoding vascular endothelial growth factor VEGF and basic fibroblast growth factor FGF2, was performed to the affected lower extremity. The effect of treatment was evaluated by functional tests: measurement of ankle-brachial index, treadmill test, the recovery time, shoulder-ankle index after strain and temporary occlusion. Performed immunohistochemical examination of biopsy samples of the affected lower extremity muscles

Combined treatment of throphic ulcer of the heel using vacuum therapy with direct gene therapy: Case report

In this article we describe a clinical case of successful treatment of neurotrophic ulcers of heel region using classical surgical techniques such as debridement and autodermoplastics combined with vacuum therapy and injection of double cassette expression plasmid encoding cDNAs of vascular endothelial growth factor and fibroblast growth factor 2. The outcome of therapy opens new horizons in the treatment of such diseases in short period of time. © Human stem cells institute, 2013

Combined treatment of throphic ulcer of the heel using vacuum therapy with direct gene therapy: Case report

In this article we describe a clinical case of successful treatment of neurotrophic ulcers of heel region using classical surgical techniques such as debridement and autodermoplastics combined with vacuum therapy and injection of double cassette expression plasmid encoding cDNAs of vascular endothelial growth factor and fibroblast growth factor 2. The outcome of therapy opens new horizons in the treatment of such diseases in short period of time. © Human stem cells institute, 2013

Combined treatment of throphic ulcer of the heel using vacuum therapy with direct gene therapy: Case report

In this article we describe a clinical case of successful treatment of neurotrophic ulcers of heel region using classical surgical techniques such as debridement and autodermoplastics combined with vacuum therapy and injection of double cassette expression plasmid encoding cDNAs of vascular endothelial growth factor and fibroblast growth factor 2. The outcome of therapy opens new horizons in the treatment of such diseases in short period of time. © Human stem cells institute, 2013

Use of stromal vascular fraction of adipose tissue for treatment of femur pseudoarthtosis: Case report

The article presents a case report of successful treatment of pseudarthrosis of the femur with the use of autologous cells of the stromal vascular fraction (SVF) derived from adipose tissue. Autologous SVF cells mixed with fibrin glue were transplanted into bone defect by injection under the control of electro-optical converter. Two months after injection, the patient reported the disappearance of pain. After 4 months we observed the recovery of function of the knee, X-ray shows signs of the fracture healing through the formation of bone callus. © Human stem cells institute, 2013