Intensity (<i>M</i>%), frequency (<i>F</i>%) of fungal colonization and arbuscules abundance (<i>A</i>%) in the roots of mycorrhized <i>Medicago truncatula</i>.

<p>Intensity (<i>M</i>%), frequency (<i>F</i>%) of fungal colonization and arbuscules abundance (<i>A</i>%) in the roots of mycorrhized <i>Medicago truncatula</i>.</p

Summary of alterations observed in mycorrhized (AM) and phosphate-fertilized (P_i) as compared to control plants.

<p>The summarized alterations are for the time points with similarly high arbuscules abundance in Experiment 1 (3 wpi) and Experiment 2 (5 wpi). ‘+’ indicates a stimulatory effect, whereas ‘No’ indicates no effect of the respective treatment as compared to control plants. It is of note that a similar pattern of alterations was observed at 3 wpi in Experiment 2. For mycorrhization conditions, see Tables <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115314#pone.0115314.t001" target="_blank">1</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115314#pone.0115314.t002" target="_blank">2</a>.</p><p>Summary of alterations observed in mycorrhized (AM) and phosphate-fertilized (P_i) as compared to control plants.</p

Supplementary information from Modulation of lipid biosynthesis by stress in diatoms

Environmental factors modify lipid and fatty acid metabolism. Table SD1. Microalgal lipid content (% of biomas) and total lipid and galactolipid EPA levels (% molar of total fatty acids) for control (22°C) and stressed cultures (10°C) of P. tricornutum after 1, 2, 4 and 8 days of temperature stress

Anatomical features of leaves from control, mycorrhized (AM) and phosphate-fertilized plants (P_i).

<p>Anatomical features of leaves from control, mycorrhized (AM) and phosphate-fertilized plants (P_i).</p

Additional file 2: Table S2. of Transcription factors in microalgae: genome-wide prediction and comparative analysis

Sources for the self-build TF database. Table listing sources for the building of the self-build transcription factor database. (XLSX 11 kb

Date of growth axis appearance for control, mycorrhized (AM) and phosphate-fertilized plants (P_i).

<p>(A) The date when each growth axis appeared was calculated as the x-intercept from linearized plots of those shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115314#pone.0115314.s001" target="_blank">S1 Fig.</a> Values with different letters for each axis are significantly different across treatments according to one-way ANOVA followed by Student-Newman-Keuls test (<i>P</i><0.05). (B) Difference in the date of growth axis appearance. The values obtained in (A) for each axis of the control plants were subtracted from the corresponding values obtained for the AM- and Pi-fertilized plants. *, Significantly different from each other. dpi, days post inoculation. For mycorrhization conditions, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0115314#pone.0115314.t001" target="_blank">Table 1</a>.</p

Additional file 1: Table S1. of Transcription factors in microalgae: genome-wide prediction and comparative analysis

Source datasets. Table listing the reference of the genomic data used in this study. (XLSX 11 kb

Dimensions of leaf chloroplasts from control, mycorrhized (AM) and phosphate-fertilized plants (P_i).

<p>Dimensions of leaf chloroplasts from control, mycorrhized (AM) and phosphate-fertilized plants (P_i).</p

Photoinhibition of PSII in Col-0, Ws-4 and L<i>er</i>-0 accessions.

<p>Leaves detached from plants grown hydroponically at an irradiance of 120 µmol photons m⁻² s⁻¹ (GL) were exposed for 3 h to GL or high light (HL = 950 µmol photons m⁻² s⁻¹) treatments in the presence or absence of lincomycin (LN). (A) The <i>F</i>_v<i>/F</i>_m parameter was determined in leaves after 5 min dark adaptation. The levels of <i>F</i>_v<i>/F</i>_m were calculated relative to a value of 0.82, obtained in 16 h dark-adapted Col-0 leaves, and plotted as means ±SD (n = 3). (B) Thylakoid membranes were isolated from the treated leaves and subjected to western blotting with anti-D1 and with anti-Lhcb2 (as loading control) (0.25 µg Chl/lane). (C) Transmission electron micrographs of chloroplast ultrastructure from plants exposed to 3 h HL. Scale bar: 1 µm.</p

Quantitative LC-MS of PSII core protein phosphorylation of Col-0, Ws-4 and L<i>er</i>-0 accessions.

<p>Data represent means ±SD of four independent preparations.</p>*<p>, Significantly different from Col-0 and L<i>er</i>-0 (Student's t-test P<0.05).</p><p>The plants were grown hydroponically for six weeks at an irradiance of 120 µmol photons m⁻² s⁻¹ and treated for 3 h at an irradiance of 950 µmol photons m⁻² s⁻¹. Thylakoid membranes were isolated in the presence of NaF. The levels of phosphorylated PSII core proteins were analyzed by quantitative mass spectrometry and expressed relative to Col-0.</p