The research of the maximum wind speed in Tomsk and calculations of dynamic load on antenna systems

The work is concerned with calculations and analysis of the maximum wind speed in Tomsk city. The data for analysis were taken from the TOR-station located in the north-eastern part of the city. The TOR-station sensors to measure a speed and a direction of wind are installed on the 10-meter meteorological mast. Wind is measured by M-63, which uses the standard approach and the program with one-minute averaging for wind gusts recording as well. According to the measured results in the research performed, the estimation of the dynamic and wind load on different types of antenna systems was performed. The work shows the calculations of wind load on ten types of antenna systems, distinguished by their different constructions and antenna areas. For implementation of calculations, we used methods developed in the Central Research and Development Institute of Building Constructions named after V.A. Kucherenko. The research results could be used for design engineering of the static antenna systems and mobile tracking systems for the distant objects

Functional Characterization of Lamina X Neurons in ex-Vivo Spinal Cord Preparation

Functional properties of lamina X neurons in the spinal cord remain unknown despite the established role of this area for somatosensory integration, visceral nociception, autonomic regulation and motoneuron output modulation. Investigations of neuronal functioning in the lamina X have been hampered by technical challenges. Here we introduce an ex-vivo spinal cord preparation with both dorsal and ventral roots still attached for functional studies of the lamina X neurons and their connectivity using an oblique LED illumination for resolved visualization of lamina X neurons in a thick tissue. With the elaborated approach, we demonstrate electrophysiological characteristics of lamina X neurons by their membrane properties, firing pattern discharge and fiber innervation (either afferent or efferent). The tissue preparation has been also probed using Ca2+ imaging with fluorescent Ca2+ dyes (membrane-impermeable or -permeable) to demonstrate the depolarization-induced changes in intracellular calcium concentration in lamina X neurons. Finally, we performed visualization of subpopulations of lamina X neurons stained by retrograde labeling with aminostilbamidine dye to identify sympathetic preganglionic and projection neurons in the lamina X. Thus, the elaborated approach provides a reliable tool for investigation of functional properties and connectivity in specific neuronal subpopulations, boosting research of lamina X of the spinal cord

Improving energy efficiency service location locomotives

В статье рассмотрена концепция повышения энергоэффективности путем применения принципов бережливого производства и возможность его внедрения в производственный процесс пункта технического обслуживания локомотивов (ПТОЛ). Сформулированы направления снижения затрат на нетяговые нужды на основе принципов бережливого производства. Проведена оценка снижения затрат при использовании сформулированных принципов.У статті розглянута концепція підвищення енергоеффективности шляхом застосування принципів бережливого виробництва і можливість його впровадження в виробничий процес пункту технічного обслуговування локомотивів (ПТОЛ). Сформульовано напрямки зниження витрат на нетягові потреби на основі принципів бережливого виробництва. Проведено оцінку зниження витрат при використанні сформульованих принципів.The article deals with the concept of energy efficiency by applying the principles of lean production and the possibility of its implementation in the production process point of maintenance of locomotives (PTOL). Formulated the direction reducing the cost of not traction needs based on the Principles of lean manufacturing. The estimation of cost reduction when using the defined principles. Lean Manufacturing - is a quality and affordable method for increasing the efficiency of the projected into the workplace, where on-usual order, increased labor and culture as a result of streamlined process facilitates the daily work of employees, increases the efficiency of production processes

Generation of versatile ss-dsDNA hybrid substrates for single-molecule analysis.

Here, we describe a rapid and versatile protocol to generate gapped DNA substrates for single-molecule (SM) analysis using optical tweezers via site-specific Cas9 nicking and force-induced melting. We provide examples of single-stranded (ss) DNA gaps of different length and position. We outline protocols to visualize these substrates by replication protein A-enhanced Green Fluorescent Protein (RPA-eGFP) and SYTOX Orange staining using commercially available optical tweezers (C-TRAP). Finally, we demonstrate the utility of these substrates for SM analysis of bidirectional growth of RAD-51-ssDNA filaments. For complete details on the use and execution of this protocol, please refer to Belan et al. (2021)

Human RAD51 rapidly forms intrinsically dynamic nucleoprotein filaments modulated by nucleotide binding state.

Formation of RAD51 filaments on single-stranded DNA is an essential event during homologous recombination, which is required for homology search, strand exchange and protection of replication forks. Formation of nucleoprotein filaments (NF) is required for development and genomic stability, and its failure is associated with developmental abnormalities and tumorigenesis. Here we describe the structure of the human RAD51 NFs and of its Walker box mutants using electron microscopy. Wild-type RAD51 filaments adopt an 'open' conformation when compared to a 'closed' structure formed by mutants, reflecting alterations in helical pitch. The kinetics of formation/disassembly of RAD51 filaments show rapid and high ssDNA coverage via low cooperativity binding of RAD51 units along the DNA. Subsequently, a series of isomerization or dissociation events mediated by nucleotide binding state creates intrinsically dynamic RAD51 NFs. Our findings highlight important a mechanistic divergence among recombinases from different organisms, in line with the diversity of biological mechanisms of HR initiation and quality control. These data reveal unexpected intrinsic dynamic properties of the RAD51 filament during assembly/disassembly, which may be important for the proper control of homologous recombination

Periodical structure of bioaerosol concentration fields in the troposphere over the southern part of West Siberia

The properties of the bioaerosol concentration fields are also influenced by oscillatory processes in the atmosphere. The analysis of data on surface concentration of aerosols of total protein obtained in the vicinity of Novosibirsk presented in allowed us to find a correlation between the variations in the aerosol mass concentration and the total protein concentration in the surface atmospheric layer with its characteristic periodical synoptic processes. In this paper we present the results of wavelet and harmonic analysis of the experimental dataset on the concentration of aerosols of total protein and viable microorganisms in the troposphere over the southern part of West Siberia obtained during a 5-yr observation period (1999-2003). Copyright © 2004 by MAIK "Nauka /Interperiodica" (Russia)

The research of the maximum wind speed in Tomsk and calculations of dynamic load on antenna systems

The work is concerned with calculations and analysis of the maximum wind speed in Tomsk city. The data for analysis were taken from the TOR-station located in the north-eastern part of the city. The TOR-station sensors to measure a speed and a direction of wind are installed on the 10-meter meteorological mast. Wind is measured by M-63, which uses the standard approach and the program with one-minute averaging for wind gusts recording as well. According to the measured results in the research performed, the estimation of the dynamic and wind load on different types of antenna systems was performed. The work shows the calculations of wind load on ten types of antenna systems, distinguished by their different constructions and antenna areas. For implementation of calculations, we used methods developed in the Central Research and Development Institute of Building Constructions named after V.A. Kucherenko. The research results could be used for design engineering of the static antenna systems and mobile tracking systems for the distant objects

Physical characteristics of concentration fields of tropospheric bioaerosols in the South of Western Siberia

The State Research Center of Virology and Biotechnology "Vector" and the Institute of Optics SB RAS are performing systematic study of tropospheric bioaerosols in the South of Western Siberia. The work summarizes the results of analysis of some physical characteristics of the data array on bioaerosol concentration obtained with an airplane laboratory at the altitudes of 0.5, 1, 1.5, 2, 3, 4, 5.5 and 7 km. The flights were performed during the last 10 days of each month in 1999-2003. It was shown that the concentration of total protein aerosols obeyed the laws of continual statistics, and the concentration of culturable microorganism aerosols obeyed the laws of discrete statistics. The analysis of correlation coefficients and cross correlation of bioaerosol concentration fields was performed. Wavelet analysis of the data showed that variations of tropospheric bioaerosol concentration were mainly determined by typical seasonal processes with periods of 12, 6, 4 and 8-9 months. Seasonal variations cause approximately 80% of the total dispersion of variations of total protein concentration, and the amplitudes of variations of culturable microorganism concentration are small as compared with the constant component. Harmonic analysis of bioaerosol concentration fields was performed. Harmonics that are significant in wavelet spectra for all time series were taken for the approximation of the series. It was revealed that four-month periodicity was internal and was caused by a wave spreading from above with a vertical rate of about 4 km/month. © 2005 Elsevier Ltd. All rights reserved

Physical characteristics of concentration fields of tropospheric bioaerosols in the South of Western Siberia

The State Research Center of Virology and Biotechnology "Vector" and the Institute of Optics SB RAS are performing systematic study of tropospheric bioaerosols in the South of Western Siberia. The work summarizes the results of analysis of some physical characteristics of the data array on bioaerosol concentration obtained with an airplane laboratory at the altitudes of 0.5, 1, 1.5, 2, 3, 4, 5.5 and 7 km. The flights were performed during the last 10 days of each month in 1999-2003. It was shown that the concentration of total protein aerosols obeyed the laws of continual statistics, and the concentration of culturable microorganism aerosols obeyed the laws of discrete statistics. The analysis of correlation coefficients and cross correlation of bioaerosol concentration fields was performed. Wavelet analysis of the data showed that variations of tropospheric bioaerosol concentration were mainly determined by typical seasonal processes with periods of 12, 6, 4 and 8-9 months. Seasonal variations cause approximately 80% of the total dispersion of variations of total protein concentration, and the amplitudes of variations of culturable microorganism concentration are small as compared with the constant component. Harmonic analysis of bioaerosol concentration fields was performed. Harmonics that are significant in wavelet spectra for all time series were taken for the approximation of the series. It was revealed that four-month periodicity was internal and was caused by a wave spreading from above with a vertical rate of about 4 km/month. © 2005 Elsevier Ltd. All rights reserved