Immunocapturing of extracellular vesicles on stainless steel for multi-modal individual characterization with correlative light, electron and probe microscopy

Here, we report a robust platform for multi-modal analysis of immuno-captured individual extracellular vesicles (EVs). Stainless steel substrates were surface-modified to covalently immobilize specific antibodies targeting proteins found on EVs. Using PDMS microchannels, EVs were selectively captured on the substrates. Next, individual EVs were retraced and correlatively characterized here using SEM, AFM and Raman Spectroscopy.</p

Immunocapturing of extracellular vesicles on stainless steel for multi-modal individual characterization with correlative light, electron and probe microscopy

Here, we report a robust platform for multi-modal analysis of immuno-captured individual extracellular vesicles (EVs). Stainless steel substrates were surface-modified to covalently immobilize specific antibodies targeting proteins found on EVs. Using PDMS microchannels, EVs were selectively captured on the substrates. Next, individual EVs were retraced and correlatively characterized here using SEM, AFM and Raman Spectroscopy.</p

Multi-modal analysis of tumor-derived extracellular vesicles immunocaptured from plasma

Extracellular vesicles have emerged in recent years as highly promising for understanding cell communication, drug delivery, and medical applications. Specifically, tumor-derived extracellular vesicles (tdEVs) have demonstrated excellent prognostic value in cancer diagnostics compared to imaging approaches. Despite the growing body of expertise regarding EVs, great challenges remain, notably in their handling and characterization. In complex media, other particles with similar characteristics may occlude measurements. Here, a platform is presented for the immunocapturing of tdEVs for identifying their origin followed by further multi-modal analysis by Raman spectroscopy, confocal microscopy and atomic force microscopy (AFM)

Multi-modal analysis of tumor-derived extracellular vesicles immunocaptured from plasma

Extracellular vesicles have emerged in recent years as highly promising for understanding cell communication, drug delivery, and medical applications. Specifically, tumor-derived extracellular vesicles (tdEVs) have demonstrated excellent prognostic value in cancer diagnostics compared to imaging approaches. Despite the growing body of expertise regarding EVs, great challenges remain, notably in their handling and characterization. In complex media, other particles with similar characteristics may occlude measurements. Here, a platform is presented for the immunocapturing of tdEVs for identifying their origin followed by further multi-modal analysis by Raman spectroscopy, confocal microscopy and atomic force microscopy (AFM)

Organosilicon interaction with biological membranes

Poly(dimethyl siloxane) (PDMS) is the number-one used material to produce microfluidic devices, under the assumption it is biocompatible. Other organosilicon compounds, including PDMS, are ubiquitous in daily use products such as cosmetics, pharmaceuticals and even food. Their approval in these applications is based on the notion that the substance is not absorbed systemically. Here, using a range of analytical techniques, we demonstrate that a range of organosilicon compounds do interact with cell membranes and models thereof.</p