Supplemental Material for Dumont et al., 2018

Supplemental figures and tables associated with manuscript entitled: Evolutionary association of sex chromosome architecture and meiotic synapsis in North American vole

Definition of the zebrafish genome using flow cytometry and cytogenetic mapping-1

<p><b>Copyright information:</b></p><p>Taken from "Definition of the zebrafish genome using flow cytometry and cytogenetic mapping"</p><p>http://www.biomedcentral.com/1471-2164/8/195</p><p>BMC Genomics 2007;8():195-195.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1925092.</p><p></p> syntenic with a previously mapped BAC clone probe, zK007C07, which localizes to the short arm (p arm) of LG chromosome 3, (labeled in orange, denoted by orange arrows). One BAC clone, zK110G19 (labeled in red), was observed to have two signals on the same chromosome, LG chromosome 4. The primary signal is on 4p with a secondary signal in the heterochromatic region of 4q. LG chromosome 4 is denoted by red arrows. Seventeen clones had signals on two non-homologous chromosomes. For example, zC211K08 (labeled in orange) localized to the p arm of LG chromosome 22 and to the q arm heterochromatic region of LG chromosome 4 (denoted by orange arrows). The near-telomeric marker for the q arm of LG chromosome 22, zC118M01, is labeled in green (denoted by green arrows) and the near-telomeric marker for the p arm of LG chromosome 4, zK030C13, is labeled in white (denoted by white arrows). Five BAC clones were pan-centromeric, such as zK171K22 (labeled in orange). Five BAC clones were peri-centromeric. For example, zK120I24 (labeled in orange) localized to the p arm and near the centromere of LG chromosome 7 (denoted by orange arrows), the p arm and near the centromere of an unknown chromosome, and near the centromere of multiple chromosomes. The near-telomeric marker for the q arm of LG chromosome 7, zC128L16, is labeled in green (denoted by green arrows)

Definition of the zebrafish genome using flow cytometry and cytogenetic mapping-2

<p><b>Copyright information:</b></p><p>Taken from "Definition of the zebrafish genome using flow cytometry and cytogenetic mapping"</p><p>http://www.biomedcentral.com/1471-2164/8/195</p><p>BMC Genomics 2007;8():195-195.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1925092.</p><p></p>as observed to have only homozygous signals near the centromere of LG chromosome 3q. zK188H10, which consistently localized near the middle of LG chromosome 3p, is labeled in green. Three hybridization patterns were observed for zK167C09 in metaphase preparations from AB embryos including homozygous signals near the centromere of LG chromosome 3q, homozygous signals located medially on LG chromosome 3p, and a heterozygous state. The heterozygous pattern is depicted in this image with zK167C09 labeled in red and zK188H10, which consistently hybridized medially on LG chromosome 3p, labeled in green. White arrows denote LG chromosome 3 in and

Definition of the zebrafish genome using flow cytometry and cytogenetic mapping-0

<p><b>Copyright information:</b></p><p>Taken from "Definition of the zebrafish genome using flow cytometry and cytogenetic mapping"</p><p>http://www.biomedcentral.com/1471-2164/8/195</p><p>BMC Genomics 2007;8():195-195.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1925092.</p><p></p>sh chromosomes. Flow-peaks contained in the oval represent the zebrafish chromosomes (see B for the chromosomal composition of each peak). The largest zebrafish LG chromosomes (5 and 7) were slightly larger than human chromosome 18 (76.12 Mb) and the smallest zebrafish LG chromosomes (22 and 25) were similar to the size of human chromosome 21 (46.94 Mb). Thus, human chromosomes 18 and 21 were chosen as references (labeled in the karyogram). A flow karyogram of the zebrafish chromosome preparation. LG chromosomes ranged in size from 42.2 to 77.9 Mb and the LG chromosomes composing each peak are denoted. The similarity of size resulted in only LG chromosomes 3, 4, and 24 separating into distinct peaks. The remaining nine peaks contained 2 to 5 chromosomes

Definition of the zebrafish genome using flow cytometry and cytogenetic mapping-3

<p><b>Copyright information:</b></p><p>Taken from "Definition of the zebrafish genome using flow cytometry and cytogenetic mapping"</p><p>http://www.biomedcentral.com/1471-2164/8/195</p><p>BMC Genomics 2007;8():195-195.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1925092.</p><p></p>gion, which made finding a near-telomeric marker probe difficult for this chromosome arm. zC079A18 (labeled in orange) was established as a near-telomeric marker probe for the q arm of LG chromosome 4 (denoted by orange arrows). Also shown is the near-centromeric marker for LG chromosome 4, zC091G03, (labeled in white, denoted by white arrows) and BAC clone zC207E19, which localizes to the heterochromatic region of the long arm of LG chromosome 4 (labeled in green, denoted by green arrows)

Definition of the zebrafish genome using flow cytometry and cytogenetic mapping-4

<p><b>Copyright information:</b></p><p>Taken from "Definition of the zebrafish genome using flow cytometry and cytogenetic mapping"</p><p>http://www.biomedcentral.com/1471-2164/8/195</p><p>BMC Genomics 2007;8():195-195.</p><p>Published online 27 Jun 2007</p><p>PMCID:PMC1925092.</p><p></p>sh chromosomes. Flow-peaks contained in the oval represent the zebrafish chromosomes (see B for the chromosomal composition of each peak). The largest zebrafish LG chromosomes (5 and 7) were slightly larger than human chromosome 18 (76.12 Mb) and the smallest zebrafish LG chromosomes (22 and 25) were similar to the size of human chromosome 21 (46.94 Mb). Thus, human chromosomes 18 and 21 were chosen as references (labeled in the karyogram). A flow karyogram of the zebrafish chromosome preparation. LG chromosomes ranged in size from 42.2 to 77.9 Mb and the LG chromosomes composing each peak are denoted. The similarity of size resulted in only LG chromosomes 3, 4, and 24 separating into distinct peaks. The remaining nine peaks contained 2 to 5 chromosomes

Fluorescence <i>in situ</i> hybridisation characterisation of the structure of the Hsa21 in Tc1 mice.

<p>(<i>a</i>) Hsa21 specific paint (green) c-hybridised with a Hsa13/21 alpha satellite centromere probe (red giving yellow signal). (<i>b</i>) Hsa21 telomere specific probe (green) co-hybridised with an Hsa13/21 alpha satellite centromere probe (red). (<i>c</i>) Human chromosome pan-telomeric probe (red) i.e. hybridises to all human and mouse pan telomere sequences, demonstrating that Hsa21 in the Tc1 is structurally altered and is metacentric.</p

Schematic of the proposed structure of Tc1-Hsa21.

<p>Reference: Ideogram of human chromosome 21, numbers 1–41 indicate regions of Tc1 Hsa21 delineated according to <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060482#pone.0060482.s010" target="_blank">Table S5</a>. Tc1: rearranged structure of the Hsa21 in Tc1 mice. The order of regions 11, 20, 22, 24, 19, 18, 25, 17, 13, 28, 26, 6, 15, 35, 32, 5, 9, 10, 33, 38, 40, 36, 37, 31, 29, 23, 22, 21, 20, and 11 in this schematic are based on FISH mapping data (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0060482#pone.0060482.s002" target="_blank">Fig. S2</a>). The certainty of the rearrangement is indicated by a red line, solid line more certain, dotted line suggested. Inverted chromosome regions are indicated by the red arrow symbol. Region 12 is triplicated but the position of the other two copies is unknown. Position of region 27 is unknown. The positions of acrocentric regions 1, 2, 3, 7 and 8 are unknown and are placed arbitrarily. Regions 26, 30 and 41 are duplications and their positions are suggested by FISH within the resolution of the technique. Regions 14, 16, 34 and 39 are deleted.</p