Free and conjugated androgen and progestin levels in the serum of stellate sturgeon (Acipenser stellatus Pallas) males treated with female coelomic fluid

Based on the supposition that female coelomic fluid contents have a certain chemical influence on sturgeons, blood serum concentrations of free and conjugated testosterone (T), 11-ketotestosterone (11KT), 17,20b,21-trihydroxy-4-pregnen- 3-one (20bS), 17,20b-dihydroxy-4-pregnen-3-one (DHP), 11-desoxycortisol (S) and free progesterone (P4) have been measured by radioimmunoassay (RIA) and enzyme-linked immuno-sorbent assay (ELISA) in stellate sturgeon (Acipenser stellatus Pallas) males treated with female coelomic fluid (CF); CF steroid levels have also been analyzed. After treatment a significant elevation of free 20bS and glucuronidated DHP and S levels and the decrease of free T and KT levels have been observed. The obtained data support the idea that the components of CF may play some part in pheromonal effects for sturgeon reproduction

Free androgens and progestins and their conjugated forms in serum and urine of stellate sturgeon (Acipenser stellatus Pallas) males

Stellate sturgeon (Acipenser stellatus Pallas) males from Volga River treated with LH-RH-A showed an increase of free, sulfated and glucuronided sex steroid levels in serum and urine at spermiation. Conjugated forms of sex steroids could act as pheromones in sturgeon

Levels of free and conjugated androgens and progestins in coelomic fluid and serum of stellate sturgeon (Acipenser stellatus Pallas) females

Stellate sturgeon (Acipenser stellatus Pallas) females from the Volga River were analysed for sex steroids after hormonal stimulation. The results show a significant increase of sulfated sex steroid levels in serum and coelomic fluid (CF) at final maturation (FM). It is hypothesized that the steroid metabolites could be part of a chemical communication system related to reproduction

Sex steroids and oocyte maturation in the sterlet (Acipenser ruthenus L.)

Sturgeon oocytes, like those of other vertebrates, are arrested in early meiosis and require a steroid or steroids to trigger meiosis resumption or maturation. The identity of the maturation-inducing steroid in sturgeons has present study was designed to investigate in sterlet the follicular secretion and relative blood changes of free and conjugated 17,20βP, 20βS and of the androgens T, 11KT, in response to hormonal treatment and to determine the relative potencies of the progestagens, 17,20β-P, 20βS, P4 and S, in inducing oocyte final maturation in vitro

Sex steroids and cortisol levels in the blood of stellate sturgeon (Acipenser stellatus Pallas) during final maturation induced by LH-RH-analogue

Blood serum cortisol (F), testosterone (T), 11-ketotestosterone (11KT), oestradiol-17 (E2), progesterone (P4), 17,20,21-trihydroxy- 4-pregnen-3-one (20βS) and 17,20β-dihydroxy-4-pregnen-3-one (17,20βP) concentrations were measured by enzyme linked immuno-sorbent assay (ELISA) and radio-immunoassay (RIA) in stellate sturgeon (Acipenser stellatus, Pallas) during final maturation (FM) induced by luteinizing hormone-releasing hormone analogue (LH-RH-A). After the LH-RH-A treatment there was a significant elevation of sex steroids (T, E2, P4, 20βS) and F levels before ovulation and decrease of T and E2 levels after ovulation. In males at the beginning of spermiation LH-RH-A induced a rise of sex steroids and F levels. There was a decrease of steroids levels at the end of spermiation. The data indicates a possible role of 20βS takes part in FM in stellate sturgeon male as well as in female. The rise in F levels may indicate an interaction between corticosteroids and gamete maturation in stellate sturgeon