An Italian Multicenter Perspective Harmonization Trial for the Assessment of MET Exon 14 Skipping Mutations in Standard Reference Samples

Lung cancer remains the leading cause of cancer deaths worldwide. International societies have promoted the molecular analysis of MET proto-oncogene, receptor tyrosine kinase (MET) exon 14 skipping for the clinical stratification of non-small cell lung cancer (NSCLC) patients. Different technical approaches are available to detect MET exon 14 skipping in routine practice. Here, the technical performance and reproducibility of testing strategies for MET exon 14 skipping carried out in various centers were evaluated. In this retrospective study, each institution received a set (n = 10) of a customized artificial formalin-fixed paraffin-embedded (FFPE) cell line (Custom METex14 skipping FFPE block) that harbored the MET exon 14 skipping mutation (Seracare Life Sciences, Milford, MA, USA), which was previously validated by the Predictive Molecular Pathology Laboratory at the University of Naples Federico II. Each participating institution managed the reference slides according to their internal routine workflow. MET exon 14 skipping was successfully detected by all participating institutions. Molecular analysis highlighted a median Cq cut off of 29.3 (ranging from 27.1 to 30.7) and 2514 (ranging from 160 to 7526) read counts for real-time polymerase chain reaction (RT-PCR) and NGS-based analyses, respectively. Artificial reference slides were a valid tool to harmonize technical workflows in the evaluation of MET exon 14 skipping molecular alterations in routine practice

Integrated Aero–Vibroacoustics: The Design Verification Process of Vega-C Launcher

The verification of a space launcher at the design level is a complex issue because of (i) the lack of a detailed modeling capability of the acoustic pressure produced by the rocket; and (ii) the difficulties in applying deterministic methods to the large-scale metallic structures. In this paper, an innovative integrated design verification process is described, based on the bridging between a new semiempirical jet noise model and a hybrid finite-element method/statistical energy analysis (FEM/SEA) approach for calculating the acceleration produced at the payload and equipment level within the structure, vibrating under the external acoustic forcing field. The result is a verification method allowing for accurate prediction of the vibroacoustics in the launcher interior, using limited computational resources and without resorting to computational fluid dynamics (CFD) data. Some examples concerning the Vega-C launcher design are shown

Towards a digital twin-based intelligent decision support for road maintenance

The digitalisation, automation and robotisation of road inspection and maintenance technologies make it possible to collect bigger volumes of data and additional types of information about road infrastructure. Methodologies and tools to support road asset management decision-making are needed to exploit this new information, progressing towards predictive maintenance and improving different aspects of road asset management. This study presents a Digital Twin-based Decision Support Tool to assist road operators in road inspection, maintenance and upgrade. The goal of the paper is twofold. First, the architecture of the Digital Twin-based Decision Support Tool is presented, describing the main components and functionalities. The system is based on a Digital Twin (DT) that mirrors real road assets to integrate different sources of data and support the processing of low-level data into high-level information. The decision support tool (DST) is able to analyse the collected information and compute the road pavement condition to derive optimal intervention plans, addressing road section conditions, human and technical resources and other external constraints. Second, the application of the proposed architecture to road pavement maintenance is described, considering the Italian highway A24 and its connections with Rome´s ring road, managed by Strada dei Parchi SpA. Road pavement data, such as the International Roughness Index (IRI) and the Sideway Force Coefficient (SFC), are integrated into the DT to be analysed through Artificial Intelligence-clustering techniques to perform the sectioning and clustering of road sections according to their status and quality index. The paper shows the benefits derived from the integration of DT technologies with DSTs for improving processes of road maintenance.This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement No 955269

An Italian Multicenter Perspective Harmonization Trial for the Assessment of MET Exon 14 Skipping Mutations in Standard Reference Samples

Lung cancer remains the leading cause of cancer deaths worldwide. International societies have promoted the molecular analysis of MET proto-oncogene, receptor tyrosine kinase (MET) exon 14 skipping for the clinical stratification of non-small cell lung cancer (NSCLC) patients. Different technical approaches are available to detect MET exon 14 skipping in routine practice. Here, the technical performance and reproducibility of testing strategies for MET exon 14 skipping carried out in various centers were evaluated. In this retrospective study, each institution received a set (n = 10) of a customized artificial formalin-fixed paraffin-embedded (FFPE) cell line (Custom METex14 skipping FFPE block) that harbored the MET exon 14 skipping mutation (Seracare Life Sciences, Milford, MA, USA), which was previously validated by the Predictive Molecular Pathology Laboratory at the University of Naples Federico II. Each participating institution managed the reference slides according to their internal routine workflow. MET exon 14 skipping was successfully detected by all participating institutions. Molecular analysis highlighted a median Cq cut off of 29.3 (ranging from 27.1 to 30.7) and 2514 (ranging from 160 to 7526) read counts for real-time polymerase chain reaction (RT-PCR) and NGS-based analyses, respectively. Artificial reference slides were a valid tool to harmonize technical workflows in the evaluation of MET exon 14 skipping molecular alterations in routine practice