Primer and probe sequences.

<p>All oligonucleotide sequences are listed in 5′–3′ orientation. M = Matrix, HA = Hemagglutinin, NA = Neuraminidase, n.a. = not applicable.</p

PCR assay validation results.

a<p>validation data was obtained on a Stratagene Mx3000 instrument for the assays indicated with an asterisk. All other assays were validated on a Stratagene Mx3005 instrument.</p>b<p>slope, efficiency (E) and correlation (R²) of standard curve; PCR efficiency was calculated as E = 10^(−1/slope)−1.</p>c<p>Limit of detection (LOD) was calculated as 95% detection probability by probit analyses applying the SPSS 17.0 Statistics software.</p>d<p>reproducibility was calculated by examination of indicated plasmid copy numbers; intraassay: sixfold examination in a single run, interassay: twofold examination of double reactions plus inclusion of intraassay data; standard deviation of obtained Ct values are listed.</p

Long-term viral shedding more than 30 days.

<p>Long-term viral shedding more than 30 days.</p

Phylogenetic tree of parainfluenza virus sequences from the fusion gene was constructed with MEGA version 5.05 using the maximum likelihood method.

<p>Patient numbers are indicated, reference sequences for parainfluenza virus type 3 selected from GenBank are indicated by their accession numbers. Bar indicates 0.1 nucleotide substitutions per site. Bootstrap values greater than 60 are displayed on branch nodes. Patient numbers of long-term virus shedding patients are indicated in bold.</p

Duration of influenza virus, parainfluenza virus and RSV shedding periods.

<p>Duration of influenza virus, parainfluenza virus and RSV shedding periods.</p

Phylogenetic tree of influenza A(H3N2) (A) (nt78-nt1061) and B/Yamagata-lineage (B) (nt100-nt571) virus sequences of the HA gene was constructed with MEGA version 5.2 using the neighbor joining method.

<p>Patient numbers are indicated, reference sequences for infuenza virus selected from GISAID are indicated by their accession numbers. Additionally, HA sequences from german national influenza sentinel are presented. The federal states are abbreviated as follows: Bayern (BAY), Baden-Württemberg (BWB), Berlin (BLN), Brandenburg (BBG), Bremen (BRE), Hamburg (HAM), Hesse (HES), Mecklenburg-Western Pomerania (MVP), Lower Saxony (NSA), North Rhine-Westphalia (NRW), Rhineland-Palatinate (RPF), Saarland (SAL), Saxony (SAS), Saxony-Anhalt (SAT), Schleswig-Holstein (SHO), Thuringia (THR). Bootstrap values greater than 70 are displayed on branch nodes.</p

Cases of influenza virus, parainfluenza virus and RSV infections in the hematology unit during the winter of 2012/2013 by week of laboratory confirmation.

<p>Cases of influenza virus, parainfluenza virus and RSV infections in the hematology unit during the winter of 2012/2013 by week of laboratory confirmation.</p

Phylogenetic trees of RSV A (A) and RSV B (B) virus sequences from the second hypervariable region of the attachment protein G gene were constructed with MEGA version 5.05 using the maximum likelihood method.

<p>Patient numbers are indicated, reference sequences for RSV A and RSV B selected from GenBank are indicated by their accession numbers. Bar indicates 0.1 nucleotide substitutions per site. Bootstrap values greater than 60 are displayed on branch nodes. Long-term RSV shedding patients are marked with a black dot.</p