Pelaksanaan alih teknologi dan alih keahlian tenaga kerja asing terhadap tenaga kerja Indonesia dihubungkan dengan undang - undang nomor 11 tahun 2020 tentang cipta kerja : Studi kasus di PT. Goodyear Indonesia Tbk

Penelitian ini di latar belakangi oleh tenaga kerja asing yang bekerja di PT. Goodyear Indonesia Tbk. yang dimana tenaga kerja asing tersebut tidak melaksanakan alih teknologi dan alih keahlian kepada tenaga kerja Indonesia pendamping. ketentuan tersebut tercantum didalam Pasal 45 Undang – Undang Nomor 11 Tahun 2020 tentang Cipta Kerja. Yang mana ketentuan tersebut menyatakan bahwa setiap tenaga kerja asing yang bekerja di Indonesia harus menunjuk tenaga kerja Indonesia pendamping yang bertujuan untuk pelaksanaan alih teknologi dan alih keahlian. Penelitian ini bertujuan untuk mengetahui pelaksanaan alih teknologi atau alih keahlian tenaga kerja asing menurut Pasal 45 point a Undang – undang Nomor 11 Tahun 2020 tentang cipta kerja di PT. GOODYEAR Indonesia Tbk., untuk mengetahui kendala alih teknologi atau alih keahlian TKA terhadap TKI di PT. GOODYEAR Indonesia Tbk., dan untuk mengetahui upaya untuk mengatasi kendala alih teknologi atau alih keahlian TKA terhadap TKI di PT. GOODYEAR Indonesia Tbk. Kerangka berpikir yang digunakan dalam penelitian ini adalah Undang – Undang Dasar 1945 Pasal 27 ayat (2), teori keadilan dan kepastian hukum, Undang – Undang Nomor 11 Tahun 2020 tentang Cipta Kerja Pasal 45, Undang – Undang Nomor 13 Tahun 2003 tentang Ketenagakerjaan Pasal 1 ayat (1) dan (2), Undang – Undang Nomor 6 Tahun 2011 tentang Keimigrasian , Peraturan Pemerintah Nomor 20 Tahun 2005 tentang alih teknologi kekayaan intelektual serta hasil kegiatan peneltian dan pengembangan oleh perguruan tinggi dan lembaga penelitian dan pengembangan, Peraturan Pemerintah Nomor 34 Tahun 2021 tentang penggunaan tenaga kerja asing, dan Peraturan Menteri Nomor 8 Tahun 2021 tentang peraturan pelaksaan peraturan pemerintah Nomor 34 Tahun 2021 tentang penggunaan tenaga kerja asing. Metode penelitian yang digunakan adalah deskriptif analitis yang menguaraikan data kualitatif yang diperoleh dari penelitian studi lapangan dan kepustakaan dengan mengguakan pendekatan hukum Yuridis Empiris dilakukan dengan mengkonsepkan keadaan masyarakat dihubungkan dengan peraturan perundang – undangan. Hasil dari penelitian ini menyimpulkan bahwa (1) PT. Goodyear Indoesia Tbk. belum melaksanakan alih teknologi dan alih keahlian dikarenakan perusahaan belum memahami regulasi baru terkait penggunaan Tenaga Kerja Asing di Indonesia yang terdapat di dalam Undang – Undang Nomor 11 Tahun 2020 tentang Cipta Kerja Pasal 45, (2) kendala yang terjadi dalam pelaksanan alih teknologi dan alih keahlian adalah kendala bahasa, kendala teknologi mesin baru, dan kendala dari pihak pemerintah yang belum mengoptimalkan pembinaan regulasi alih tekonlogi dan alih keahlian, (3) upaya yang dilakukan untuk untuk mengatasi kendala tersebut yaitu dengan melaksanakan pelatihan bahasa, dan juga mengoptimalkan proses pembinaan dari pemerintah kepada perusahaan

SELF DISCLOSURE DALAM KOMUNIKASI INTERPERSONAL: KESETIAAN, CINTA, DAN KASIH SAYANG

Self disclosure dalam komunikasi interpersonal sangat berpengaruh bukan hanya pada  kehidupan sehari-hari tetapi juga di masa yang akan datang. Manusia merupakan makhluk sosial yang tidak bisa bergantung hidup sendiri, pun manusia mempunyai fitrah tersendiri yang diciptakan Tuhan, seperti kesetiaan, cinta dan kasih sayang. Hal itu sangat penting ketika berkomunikasi berlangsung. Self disclosure sendiri juga berarti keterbukaan diri yang mana setiap makhluk sosial yakni manusia harus mempunyai keterbukaan dirinya. Setiap manusia seringkali menutupi atau merahasiakan informasi dirinya terhadap orang lain, sehingga sebagian manusia tidak percaya akan kepada dirinya sendiri. Melalui komunikasi individu dapat memenuhi kebutuhan emosional dan meningkatkan kesehatan mentalnya. Belajar untuk memaknai apa itu kesetiaan, cinta dan kasih sayang pun sangat perlu karena dapat seseorang dapat mengalami berbagai macam kualitas perasaan dan membandingkan perasaan dengan yang lainnya. Kata Kunci: Self Disclosure, Komunikasi Interpersonal, Kesetiaan, Cinta, dan Kasih Sayan

Validation of the Detection Kit for Pathogenic Bacteria Salmonella typhi Causes Food Poisoning with Real Time Polymerase Chain Reaction

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototype detection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60ºC and a standard DNA concentration of 50 ng/µL. The results of the Real Time PCR confirmation test of Salmonella typhi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to thestandard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistentand reproducible data

Validation of the Detection Kit for Pathogenic Bacteria Salmonella typhi Causes Food Poisoning with Real Time Polymerase Chain Reaction

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis oftyphoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototypedetection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60ºC and a standard DNA concentration of 50 ng/µL. The results of the Real Time PCR confirmation test of Salmonellatyphi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to thestandard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistentand reproducible data

Validation of the Detection Kit for Pathogenic Bacteria

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis oftyphoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototypedetection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60ºC and a standard DNA concentration of 50 ng/µL. The results of the Real Time PCR confirmation test of Salmonellatyphi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to thestandard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistentand reproducible data

Validation of the Detection Kit for Pathogenic Bacteria

Salmonella typhi is a gram-negative bacteria that causes food poisoning. Salmonella typhi bacteria can cause typhus, which can produce lipopolysaccharide complex endotoxin, which plays an important role in the pathogenesis of typhoid fever. Cases of food poisoning are still common and are one of the causes of death and death in Indonesia. In 2019, there were 77 cases of food poisoning in Indonesia. A fast, accurate, and specific detection method is needed to detect poisoning. In previous studies, the optimum conditions and formulas for detecting these bacteria have been obtained using Real Time PCR. The results of previous studies are used as the basis for the development of a prototype detection kit. In this study, validation was carried out, which aimed to confirm the results of previous studies so that a reproducible and accurate product for the detection of Salmonella typhi bacteria could be obtained. The results of this study showed that fim-C primers for Salmonella typhi were amplified at 95 base pairs (bp) with an annealing temperature of 60ºC and a standard DNA concentration of 50 ng/µL. The results of the Real Time PCR confirmation test of Salmonella typhi bacteria at a pre-denaturation of 3 minutes with a concentration of 10 pmol obtained the Ct value according to thestandard with previous studies. The Ct value obtained was 13.96 for S. typhi bacteria. Based on the results obtained, it can be conclude that the condition validation stage for pure cultures was successfully carried out by producing consistentand reproducible data