Ectomycorrhizal fungal communities on seedlings and conspecific trees of Pinus mugo grown on the coastal dunes of the Curonian Spit in Lithuania

Ectomycorrhizal (ECM) communities of mature trees and regenerating seedlings of a non-native tree species Pinus mugo grown in a harsh environment of the coastal region of the Curonian Spit National Park in Lithuania were assessed. We established three study sites (S1, S2, and S3) that were separated from each other by 15 km. The ECM species richness was rather low in particular for mature, 100-year-old trees: 12 ectomycorrhizal taxa were identified by molecular analysis from 11 distinguished morphotypes. All 12 taxa were present on seedlings and on mature trees, with between 8–11 and 9–11 taxa present on seedlings and mature trees, respectively. Cenococcum geophilum dominated all ECM communities, but the relative abundance of C. geophilum mycorrhizas was nearly two times higher on seedlings than on mature trees. Mycorrhizal associations formed by Wilcoxina sp., Lactarius rufus, and Russula paludosa were also abundant. Several fungal taxa were only occasionally detected, including Cortinarius sp., Cortinarius obtusus, Cortinarius croceus, and Meliniomyces sp. Shannon’s diversity indices for the ECM assemblages of P. mugo ranged from 0.98 to 1.09 for seedling and from 1.05 to 1.31 for mature trees. According to analysis of similarity, the mycorrhizal communities were similar between the sites (R = 0.085; P = 0.06) and only slightly separated between seedlings and mature trees (R = 0.24; P < 0.0001). An incidental fruiting body survey that was conducted weakly reflected the below-ground assessment of the ECM fungal community and once again showed that ECM and fruiting body studies commonly supply different partial accounts of the true ECM fungal diversity. Our results show that P. mugo has moved into quite distinct habitats and is able to adapt a suite of ECM symbionts that sufficiently support growth and development of this tree and allow for natural seedling regeneration

Skirtingų paprastosios pušies (Pinus Sylvestris L.) populiacijų palikuonių (Sėjinukų) augimo ypatumai skirtinguose klimatiniuose rajonuose

Vytauto Didžiojo universitetasŽemės ūkio akademij

Mikorizacijos ypatumai auginant skirtingų kilmių paprastosios pušies ( Pinus sylvestris L.) sodmenis

Vytauto Didžiojo universitetasŽemės ūkio akademij

Mikorizės ypatumai auginant skirtingų populiacijų paprastosios pušies (Pinus Sylvestris L.) sodmenis

Vytauto Didžiojo universitetasŽemės ūkio akademij

Miško sodmenų auginimo naujovės Lenkijoje

Vytauto Didžiojo universitetasŽemės ūkio akademij

Perspektywy i problemy w zakresie zwiększenia lesistości Litwy

Vytauto Didžiojo universitetasŽemės ūkio akademij

Substratų įtaka paprastosios pušies sėjinukų mikorizei daigynuose

Vytauto Didžiojo universitetasŽemės ūkio akademij

Growth and mycorrhizal community structure of Pinus sylvestris seedlings following the addition of forest litter

We report the effects of pine and oak litter on species composition and diversity of mycorrhizal fungi colonizing 2-year-old Pinus sylvestris L. seedlings grown in a bare-root nursery in Lithuania. A layer of pine or oak litter was placed on the surface of the nursery bed soil to mimic natural litter cover. Oak litter amendment appeared to be most favorable for seedling survival, with a 73% survival rate, in contrast to the untreated mineral bed soil (44%). The concentrations of total N, P, K, Ca, and Mg were higher in oak growth medium than in pine growth medium. Relative to the control (pH 6.1), the pH was lower in pine growth medium (5.8) and higher in oak growth medium (6.3). There were also twofold and threefold increases in the C content of growth medium with the addition of pine and oak litter, respectively. Among seven mycorrhizal morphotypes, eight different mycorrhizal taxa were identified: Suillus luteus, Suillus variegatus, Wilcoxina mikolae, a Tuber sp., a Tomentella sp., Cenococcum geophilum, Amphinema byssoides, and one unidentified ectomycorrhizal symbiont. Forest litter addition affected the relative abundance of mycorrhizal symbionts more than their overall representation. This was more pronounced for pine litter than for oak litter, with 40% and 25% increases in the abundance of suilloid mycorrhizae, respectively. Our findings provide preliminary evidence that changes in the supply of organic matter through litter manipulation may have far-reaching effects on the chemistry of soil, thus influencing the growth and survival of Scots pine seedlings and their mycorrhizal communitiesVytauto Didžiojo universitetasŽemės ūkio akademij

Mikorizės įtaka skirtingų paprastosios pušies (Pinus Sylvestris L.) provenencijų palikuonių (sėjinukų) išauginimui lauko daigynuose

Santr. anglVytauto Didžiojo universitetasŽemės ūkio akademij

Mikrorizės įtaka skirtingų paprastosios pušies (Pinus sylvestris L.) provenencijų palikuonių (sėjinukų) išauginimui lauko daigynuose

Vytauto Didžiojo universitetasŽemės ūkio akademij