2 research outputs found
Crystal Growth of Aspirin Using a Temperature-Controlled Microfluidic Device
Identifying
the most appropriate polymorph of active pharmaceutical ingredients
is one of the important steps in drug development, since their bioactivities
are largely dependent on their solid forms. However, the sample preparation
for the characterization of crystal forms is time-consuming and requires
large quantities of sample. Here, we introduce a microfluidic device-based
method to prepare a sub-millimeter-sized single aspirin crystal from
a small quantity of material. For the crystal preparation, a device
equipped with a solution flow system and temperature controller was
placed under the microscope. To use the device, concentration–temperature
phase diagrams were generated, and regions where dominant nucleation
or crystal growth with specific directions were clearly determined.
By observing time-dependent changes of crystal number and size with
solution temperature, a pathway to grow a single crystal of aspirin
was determined and applied to prepare a sub-millimeter-sized crystal
from 250 μg of aspirin. The obtained crystal was sufficiently
large for single-crystal X-ray diffraction analysis, which usually
requires 10 mg to 1 g of material per crystallization experiment.
Thus, this method can be adapted as an efficient approach to uncovering
the crystallization process to obtain required crystal forms with
minimal sample consumption
A Bioinspired Coprecipitation Method for the Controlled Synthesis of Magnetite Nanoparticles
Nature often uses precursor phases
for the controlled development
of crystalline materials with well-defined morphologies and unusual
properties. Mimicking such a strategy in in vitro model systems would
potentially lead to the water-based, room-temperature synthesis of
superior materials. In the case of magnetite (Fe<sub>3</sub>O<sub>4</sub>), which in biology generally is formed through a ferrihydrite
precursor, such approaches have remained largely unexplored. Here
we report on a simple protocol that involves the slow coprecipitation
of Fe<sup>III</sup>/Fe<sup>II</sup> salts through ammonia diffusion,
during which ferrihydrite precipitates first at low pH values and
is converted to magnetite at high pH values. Direct coprecipitation
often leads to small crystals with superparamagnetic properties. Conversely,
in this approach, the crystallization kineticsî—¸and thereby
the resulting crystal sizesî—¸can be controlled through the NH<sub>3</sub> influx and the Fe concentration, which results in single
crystals with sizes well in the ferrimagnetic domain. Moreover, this
strategy provides a convenient platform for the screening of organic
additives as nucleation and growth controllers, which we demonstrate
for the biologically derived M6A peptide