Infrastructure, Production, and Archive: American and Japanese Video Art Production of 1960s and 1970s

Focusing a study on the infrastructure of artistic production and maintenance opens a space in which to examine the relationship between artistic inspiration and knowledge making, the occurrences and the writing of its history. In the case of the comparative study of the emergence of Japanese and American video art, common artistic technique employed may indicate motivations derived from the technical possibility of the video medium, while the study of infrastructure demonstrates how large-scale funding, formation of archives, the establishment of systems of distribution and channels of education affect the emergence and development of video art as a genre. This thesis analyzes the synchronous and productive rise of video art in the U.S. and Japan from mid-1960s through mid-1970s, while problematizing the systems of knowledge formation and cultural maintenance that produced a different reception of the histories. In reviewing the structure of cultural maintenance today, the digital field inspires an exploration of new avenues for models of infrastructure that is alternative to traditional institutional frameworks. The digital platform as a pedagogical tool, research resource, and discussion forum allows cross-linguistic understanding, networked scholarship, and open accessibility, suggesting an ideal method for knowledge making. Moreover, this thesis suggests the digital platform as a potential catalyst for a collaborative administration of archive and preservation of Japanese experimental film and video, proposing an alternative method of knowledge formation and cultural maintenance moving forward

Nuclear Access and Action of Notch In Vivo

AbstractThe Drosophila Notch (N) gene encodes a conserved single-pass transmembrane receptor that transduces extracellular signals controlling cell fate. Here, we present evidence that the intracellular domain of Notch gains access to the nucleus in response to ligand, possibly through a mechanism involving proteolytic cleavage and release from the remainder of the protein. In addition, our results suggest that signal transduction by Notch depends on the ability of the intracellular domain, particularly the portion containing the CDC10 repeats, to reach the nucleus and to participate in the transcriptional activation of downstream target genes

Crystal structures of Lymnaea stagnalis AChBP in complex with neonicotinoid insecticides imidacloprid and clothianidin

Neonicotinoid insecticides, which act on nicotinic acetylcholine receptors (nAChRs) in a variety of ways, have extremely low mammalian toxicity, yet the molecular basis of such actions is poorly understood. To elucidate the molecular basis for nAChR–neonicotinoid interactions, a surrogate protein, acetylcholine binding protein from Lymnaea stagnalis (Ls-AChBP) was crystallized in complex with neonicotinoid insecticides imidacloprid (IMI) or clothianidin (CTD). The crystal structures suggested that the guanidine moiety of IMI and CTD stacks with Tyr185, while the nitro group of IMI but not of CTD makes a hydrogen bond with Gln55. IMI showed higher binding affinity for Ls-AChBP than that of CTD, consistent with weaker CH–π interactions in the Ls-AChBP–CTD complex than in the Ls-AChBP–IMI complex and the lack of the nitro group-Gln55 hydrogen bond in CTD. Yet, the NH at position 1 of CTD makes a hydrogen bond with the backbone carbonyl of Trp143, offering an explanation for the diverse actions of neonicotinoids on nAChRs