Association of adipokines, insulin resistance, hypertension and dyslipidemia in patients with psoriasis vulgaris

Background: Systemic inflammation in psoriasis causes insulin resistance and cardiovascular diseases. Adipokines are adipose-tissue-derived factors that are involved in metabolic processes. It is thought that these adipokines are associated with the development of psoriasis. Objective: The purpose of this study was to determine the changes in adipokine levels, insulin resistance, hypertension, and dyslipidemia over a 12-week period. Methods: The study comprised 35 psoriasis patients and 50 controls. Blood samples were obtained twice from the patients, one sample at the start and one at the end of a 12-week follow-up period. The following parameters were assessed in both groups: serum fasting glucose, fasting insulin, homeostasis model assessment-estimated insulin resistance (HOMA-IR) index, serum lipids, adiponectin, leptin, resistin, chemerin, omentin, vaspin, visfatin, retinol-binding protein 4, and high-sensitivity C-reactive protein (hs-CRP) levels; blood pressure; body mass index; and the psoriasis area severity index (PASI) scores. Results: The patients showed an improvement in the PASI score and a significant decrease in serum hs-CRP, omentin, and chemerin values. Moreover, at the start of the follow-up, the psoriasis patients had significantly lower levels of adiponectin and visfatin and significantly higher levels of vaspin and resistin than those of the control group. Visfatin levels correlated negatively with low-density lipoprotein (LDL) and cholesterol, while vaspin and omentin levels correlated positively with diastolic blood pressure. Decreased adiponectin levels correlated negatively with diastolic blood pressure and LDL. Conclusion: Plasma levels of adipokines might be useful for evaluating the disease activity of psoriasis and its comorbidities

Leptin receptor gene polymorphism may affect subclinical atherosclerosis in patients with acromegaly

Background: Acromegaly is associated with increased morbidity and mortality related to cardiovascular diseases. Leptin (LEP) and Leptin Receptor (LEPR) gene polymorphisms can increase cardiovascular risks. The aim of this study was to investigate association between the frequencies of LEP and LEPR gene polymorphisms and subclinical atherosclerosis in acromegalic patients. Methods: Forty-four acromegalic patients and 30 controls were admitted to study. The polymorphisms were identified by using polymerase chain reaction from peripheral blood samples. The levels of systolic and diastolic blood pressure, BMI, fasting plasma glucose, fasting insulin, IGF-I, GH, IGFBP3, leptin, triglyceride, carotid Intima Media Thickness (cIMT) and HDL and LDL cholesterol concentrations were evaluated. Results: There was statistically significant difference between the LEPR genotypes of acromegalic patients (GG 11.4%, GA 52.3%, and AA 36.4%) and controls (GG 33.3%, GA 50%, and AA 16.7%) although their LEP genotype distribution was similar. In addition, the prevalence of the LEPR gene G and A alleles was significantly different between patients and controls. No significant difference was found among the G(-2548) A leptin genotypes of groups in terms of the clinical parameters. cIMT significantly increased homozygote LEPR GG genotype group compared to AA subjects in patients. But the other parameters were not different between LEPR genotypes groups of patients and controls. Conclusion: It can be said that the LEPR gene polymorphism may affect cIMT in patients. The reason is that LEPR GG genotype carriers may have more risk than other genotypes in the development of subclinical atherosclerosis in acromegaly. © 2016, Avicenna Journal of Medical Biotechnology. All rights reserved

Leptin Receptor Gene Polymorphism may Affect Subclinical Atherosclerosis in Patients with Acromegaly

Abstract Background: Acromegaly is associated with increased morbidity and mortality related to cardiovascular diseases. Leptin (LEP) and Leptin Receptor (LEPR) gene polymorphisms can increase cardiovascular risks. The aim of this study was to investigate association between the frequencies of LEP and LEPR gene polymorphisms and subclinical atherosclerosis in acromegalic patients. Methods: Forty-four acromegalic patients and 30 controls were admitted to study. The polymorphisms were identified by using polymerase chain reaction from peripheral blood samples. The levels of systolic and diastolic blood pressure, BMI, fasting plasma glucose, fasting insulin, IGF-I, GH, IGFBP3, leptin, triglyceride, carotid Intima Media Thickness (cIMT) and HDL and LDL cholesterol concentrations were evaluated. Results: There was statistically significant difference between the LEPR genotypes of acromegalic patients (GG 11.4%, GA 52.3%, and AA 36.4%) and controls (GG 33.3%, GA 50%, and AA 16.7%) although their LEP genotype distribution was similar. In addition, the prevalence of the LEPR gene G and A alleles was significantly different between patients and controls. No significant difference was found among the G(-2548) A leptin genotypes of groups in terms of the clinical parameters. cIMT significantly increased homozygote LEPR GG genotype group compared to AA subjects in patients. But the other parameters were not different between LEPR genotypes groups of patients and controls. Conclusion: It can be said that the LEPR gene polymorphism may affect cIMT in patients. The reason is that LEPR GG genotype carriers may have more risk than other genotypes in the development of subclinical atherosclerosis in acromegaly

The effect of ileal interposition on plasma glucagon like peptide-1 (glp-1) level and pancreas glp-1 receptor expression in metabolic syndrome rats

Metabolik sendrom (MeTs); diyabet, kardiyovasküler hastalık ve erken ölüm riskini arttıran bir dizi faktörü temsil etmektedir. MeTs’in ortaya çıkma sebebi obezite ile birlikte değişen gatrointestinal hormonlar olabilir. Metabolik cerrahi bu hormonlar üzerine etkili olan bir tedavi yöntemidir ve ince bağırsak kaynaklı hormonları (inkretin) düzenlemeyi hedeflemektedir. İnkretin hormonlar, oral besin alımına yanıt olarak enteroendokrin hücreler tarafından salgılanır ve pankreas β hücrelerinden glikoz kaynaklı insülin sekresyonunu uyarmaktadırlar. Amacımız, MeTs'i tanımlayan parametreler üzerine bir metabolik cerrahi tipi olan ileal interpozisyon (IT)’nin etkilerini araştırmaktır. Çalışmamızda 5 aylık hayvanların (sıçan) MeTs parametrelerini sağladıklarını gösterdikten sonra, MeTs grubu hayvanları 3 gruba ayrıldı (MeTs, Sham ve IT). MeTs, ilgili gruplara monosodyum glutamat (MSG)’ın (4mg/g) subkutan verilmesiyle oluşturuldu. Sonuç olarak, MeTs’li sıçanlarda IT’nin; hiperinsülinemiyi iyileştirdiği, lipit profili düzelttiği, obezite indeksi ve insülin direncini normalize ettiği gösterildi. IT, distal bağırsaktaki L hücrelerinden inkretin hormon olan Glukagon Like Peptit-1 (GLP-1) salgısını etkilemedi, pankreas GLP-1R ekspresyon düzeyini ise arttırdı. Elde ettiğimiz veriler doğrultusunda, pankreas GLP-1R ekspresyonundaki artışın MeTs kriterlerinin düzelmesinde önemli bir rol oynayabileceği düşünülebilir. Bu konuda daha detaylı araştırmalara ihtiyaç vardır.The metabolic syndrome (MeTs) is a cluster of risk factors indicating an increased risk of diabetes, cardiovascular disease and premature mortality. The cause of MeTs may be the hormones that change with obesity. Metabolic surgery is an effective treatment for these hormones. Metabolic surgery is an effective treatment for these hormones and aims to regulate small intestinal hormones (incretin). Incretin hormones are secreted by enteroendocrine cells in response to oral nutrient ingestion and stimulate glucose-induced insulin secretion from pancreatic β-cells. Our aim is to investigate the effects of ileal interposition (IT), a type of metabolic surgery, on the parameters defining MeTs. In the present study, MeTs group animals were divided into 3 groups (MeTs, Sham and IT). MeTs were formed by subcutaneous administration of MSG (4 mg/kg) to related groups. IT did not affect the secretion of Glucagon Like Peptide-1 (GLP-1) from the L cells in the distal bowel and increased the expression level of the pancreas GLP-1R. Based on our data, it can be thought that increased pancreatic GLP-1R expression may play an important role in the improvement of MeTs criteria. Further research is needed on this subject

Effect of erythropoietin and stem cells at experimental traumatic brain injury

Bu tez, Pamukkale Üniversitesi Bilimsel Araştırma Projeleri tarafından desteklenen, 2013SBE004 nolu proje kapsamında gerçekleştirilmiştir. Bu tezin yapılmasına Pamukkale Üniversitesi Tıbbi Etik Kurulu tarafından onay verilmiştir (PAUHDEK-2012/027).Travmatik beyin hasarı (TBH)'nda eritropoetin ve kök hücrelerin iyileşmedeki etkisini araştırmayı amaçlayan bu çalışmada deney hayvanı olarak ortalama ağırlığı 200-250 gram olan 6-8 aylık 29 adet Wistar Albino cinsi sıçan kullanılmıştır. Deney hayvanları rastgele ayrılarak 4 ayrı çalışma grubu oluşturulmuştur; Kontrol (K), Eritropoetin (EPO), Kök hücre (KH), Kök hücre+Eritropoetin (KH+EPO). K grubuna sadece TBH yapılmıştır. EPO grubuna TBH'dan yarım saat sonra intraperitoneal (i.p.) 1000 U/kg EPO verilmiştir. KH grubuna hasar bölgesine 3x104 miktarında CD34+ kök hücre süspansiyonu verilmiştir. KH+EPO grubuna ise TBH'dan yarım saat sonra i.p. olarak 1000 U/kg EPO ve hasar bölgesine 3x104 miktarında CD34+ kök hücre süspansiyonu uygulanmıştır. TBH oluşturmadan önce ve hasar oluşturduktan sonra yedi haftalık takip boyunca sıçanların motor koordinasyon ve performans değerlendirmesi rotarod performans testi ve eğik düzlem testi ile yapılmıştır. Yedi haftalık iyileşme takibi sonunda beyin dokuları radyolojik ve histopatolojik açıdan değerlendirilmiştir. TBH sonrasında gruplar arasında rotarod performans test sonuçları değerlendirildiğinde gruplar arasında istatistiksel olarak anlamlı farklılık bulunmamıştır. KH+EPO grubunda K, KH ve EPO grubuna göre, KH grubunda K ve EPO grubuna göre, EPO grubunda ise K grubuna göre eğik düzlem test sonuçları değerlendirildiğinde gruplar arasındaki fark istatistiksel olarak anlamlıdır. KH+EPO grubunda K, KH ve EPO grubuna göre, KH grubunda K ve EPO grubuna göre, EPO grubunda ise K grubuna hasar iyileşmesinin daha iyi olduğu hem histolojik hem de radyolojik bulgularda görülmüştür. Bu çalışmanın sonucunda KH ve EPO ayrı ayrı verilmesinin nörolojik fonksiyon ve hasar iyileşmesi üzerinde olumlu bir etkiye sahip olduğunu söyleyebiliriz. Buna ilaveten bulgularımız birlikte verilmelerinin iyileşmedeki olumlu etkilerinin tek tek verilmelerinden daha güçlü olduğunu göstermektedir.In this study, we aimed to investigate the effect of healing of erythropoietin and stem cells in traumatic brain injury (TBI), Twenty nine wistar albino rats who had the average weight 200-250 g in 6-8 month were used. The experimental animals were randomly divided into four different working groups: Control (C), Erythropoietin (EPO), Stem cells (SC) and Stem cell + Erythropoietin (SC+EPO). K group was created only TBI. In EPO group, 1000 U/kg EPO was given intraperitoneally at the 30 minutes after TBI. Immediately after formation TBI amount of 3X104 CD34+ stem cell suspension was injected directly onto the defect area. Immediately after formation TBI in an amount of 3X104 CD34+ stem cell suspension to the defect area+half an hour after TBI i.p. 1000 U/kg EPO were injected. Before creating TBI and after damage during seven weeks experimental damage period to rats for the measurement of motor coordination- performance was performed rotarod performance and inclined plane test. At the end of the seven-week experimental period brain tissues evaluated in the sense of were radiological and histopathological. Rotarod performance test did not change remarkably even after the injury. KH+EPO group compared to K, EPO and KH groups, KH group compared to K, EPO groups, EPO group compared to K, the were found positively statistically significant differences in the inclined plane test results and healing damage. As a result of this study we can say that separately given KH and EPO have positive effect on the healing damage and neurological function. Furthermore, our findings suggests that their administration is more powerfull than their coadministration in the positive effects in healing