Flow cytometry data of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice

<p>Data 1. Flow cytometry analysis of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice.<br>Spleen cells were obtained from wild type (A and D), 3H9-transgenic (B and E), and CD40L/3H9 double transgenic mice (C and F). Cells were stained with Alexa Fluor 647-conjugated (FL 8 channel) rat anti-mouse B220 and Pacific Blue-conjugated (FL 6 channel) anti-mouse Ig λ1(LS-136), and with (D-F) or without (A-C) FITC-conjugated (FL 1 channel) goat anti-mouse Ig λ chain. Lymphoid cells were gated by FSC/SSC dot plots and the B220+ cells were analyzed on a CyAn ADP flow cytometer (Beckman Coulter, USA).</p

Microscopy data of spleen cells from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice

<p>Data 2. Fluorescent microscopic analysis of spleen sections from wild type, 3H9-transgenic and CD40L/3H9 double transgenic mice.<br>Spleen sections were obtained from wild type (A), 3H9-transgenic (B), and CD40L/3H9 double transgenic mice (C). Tissue sections were stained with biotin-conjugated rat anti-mouse MOMA-1, Alexa Fluor 647- conjugated streptavidin (red), Pacific Blue-conjugated rat anti-mouse B220 (green) and FITC-conjugated goat anti-mouse λ chain (blue) antibody. Fluorescent images were obtained with a Zeiss LSM 510 META laser scanning confocal microscope.</p