Design and evaluation of Actichip, a thematic microarray for the study of the actin cytoskeleton-0

<p><b>Copyright information:</b></p><p>Taken from "Design and evaluation of Actichip, a thematic microarray for the study of the actin cytoskeleton"</p><p>http://www.biomedcentral.com/1471-2164/8/294</p><p>BMC Genomics 2007;8():294-294.</p><p>Published online 29 Aug 2007</p><p>PMCID:PMC2077341.</p><p></p>3 replicates per RNA sample), the human oligonucleotide Operon set (4 replicates) and the Actichip arrays (10 replicates). Experiments were carried out using the same lots of RNAs extracted from human carcinoma MCF-7 cell line and from human skeletal muscle. Data were analysed as stated in the "Methods" section resulting in two groups of genes : "detected" and "not detected". The genes found similarly expressed or not expressed in less than 2/3 of the replicated assays were deemed "non reproducible". The histograms show the distribution of the three groups of genes for each platform relative to the samples. Results are expressed as percentages relative to the total number of genes simultaneously represented on each array platform

Legislative Documents

Also, variously referred to as: House bills; House documents; House legislative documents; legislative documents; General Court documents

Additional file 3 of Predicting clinical outcome of neuroblastoma patients using an integrative network-based approach

aCGH data processing. This file describes the processing of the aCGH dataset. (PDF 1773 kb

α7/5-HT_3A CNiFER: Methyllycaconitine antagonism.

<p>A. Competitive blockade of (±)-epibatidine elicited responses on α7/5-HT_3A CNiFERs by MLA. The EC₅₀ values for each curve shifted from 86 nM to 230 nM, 900 nM, and 8.3 µM for 3 nM, 10 nM, 30 nM MLA respectively. B. Schild plot of concentration dependent shifts. A slope of −1.8±0.2 shows MLA to block competitively but with a percentage of receptors still occupied, attributing to a slight non-competitive component that may be an artifactual result of slow dissociation.</p

Comparison of Antagonist K_a from CNiFERs to non-CNiFERs detection^f.

<p>a. Measured with 10 µM PNU-120596 in aCSF.</p><p>b. Measured in aCSF.</p><p>c. Measured with potential-sensitive dye (to be published Kuryatov A, Mukherjee J, and Lindstrom J). *(2α3β) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016519#pone.0016519-Kuryatov2" target="_blank">[40]</a>.</p><p>d. Measured with Ca²⁺ dye (Fluo-3-AM) on HEK293 cells expressing human receptors <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016519#pone.0016519-Craig1" target="_blank">[23]</a>. *(Geometric Mean [-SD = 66, +SD = 109])<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016519#pone.0016519-ChavezNoriega1" target="_blank">[39]</a>.</p><p>e. Measured competition against [³H]-ICs-205-930 in mouse neuroblastoma-glioma cells (NG-108-15) (5-HT_3AB)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016519#pone.0016519-Rizzi1" target="_blank">[41]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016519#pone.0016519-Rosen1" target="_blank">[42]</a>.</p><p>f. Each n is calculated from a Schild regression plot.</p

Simplistic representation of LGIC-CNiFERs and their response.

<p>Upon activation of a calcium permeable receptor, Ca²⁺ enters the cell and upon binding to TN-XXL produces a conformational change eliciting a fluorescence change reflected in quenching of the donor fluorescence and excitation of the acceptor.</p

α4β2-nAChR CNiFER: DHβE antagonism.

<p>A. Competitive blockade of (±)-epibatidine elicited responses on α4β2-nAChR CNiFERs by DHβE. The EC₅₀ values for each curve shifted from 8.9 nM to 42 nM, 130 nM, and 270 nM for 300 nM, 1 µM, 3 µM DHβE respectively. B. Schild plot of concentration dependent shifts. A slope of −0.9±0.1 confirmed DHβE to block competitively.</p

Comparison of Agonist EC₅₀ using CNiFER and non-CNiFER detection^f.

<p>a. Measured in aCSF.</p><p>b. Measured with Ca²⁺ dye (Fluo-3-AM) on HEK293 cells expressing human receptors <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016519#pone.0016519-Craig1" target="_blank">[23]</a> *(Geometric Mean[-SD = 2.4, +SD = 5.1])<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016519#pone.0016519-ChavezNoriega1" target="_blank">[39]</a>.</p><p>c. Measured with 10 µM PNU-120596 in aCSF.</p><p>d. Measured with potential-sensitive dye against human receptors. *(2α3β) <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016519#pone.0016519-Kuryatov1" target="_blank">[35]</a>.</p><p>e. Measured with potential-sensitive dye and 3 µM PNU-120596.</p>†<p>To be published Kuryatov A, Mukherjee J, and Lindstrom J.</p><p>f. Each n is calculated as an EC₅₀ value from an individual concentration-response curve.</p

5-HT_3A CNiFER: Ondansetron antagonism.

<p>A. Competitive blockade of 5-HT elicited responses on 5-HT_3A CNiFERs by ondansetron. The steep Hill coefficient of 2.6 shows the more cooperative nature of the 5-HT_3A receptor. The EC₅₀ values for each curve shifted from 220 nM to 1.6 µM, 2.6 µM, and 4.5 µM for 5 nM, 10 nM, 15 nM ondansetron respectively. B. Schild plot of concentration dependent shifts. A slope of -1.3±0.04 confirmed ondansetron to block competitively.</p

Evidence of the presence of morphine-like immunoreactivity in secretory granules.

<p><i>A</i>. <i>Upper panel</i>, double immunofluorescence confocal micrographs. Labeling was performed with a sheep anti-morphine antibody (visualized in green with an Alexa Fluor 488-conjugated IgG) and with an antibody against CGA (a specific intragranular marker) visualized in red with a mouse Cy3-conjugated IgG. Colocalized immunolabelling (merged window) appears as yellow staining. <i>Lower panel</i>, SH-SY5Y termini shown at higher magnification. Arrows indicate colocalization points. An identical pattern of labelling was obtained for a different mouse monoclonal anti-morphine antibody (data not shown). <i>B.</i> To assess the specificity of morphine immunolabelling, control experiments were performed using either sheep non-immune serum and an Alexa Fluor 488-conjugated IgG, or Alexa Fluor 488-conjugated IgG and Cy3-conjugated IgG without a primary antibody.</p