Additional file 1: Figure S1. of Optimization of proximity ligation assay (PLA) for detection of protein interactions and fusion proteins in non-adherent cells: application to pre-B lymphocytes

PLA with three different pairs of antibodies against ETV6 and RUNX1. Quantification of PLA signals per nucleus in REH cells. Three different pairs of antibodies against ETV6 and RUNX1 were tested as indicated in the figure. (PDF 77 kb

Five distinct biological processes and 14 differentially expressed genes characterize -positive leukemia-0

<p><b>Copyright information:</b></p><p>Taken from "Five distinct biological processes and 14 differentially expressed genes characterize -positive leukemia"</p><p>http://www.biomedcentral.com/1471-2164/8/385</p><p>BMC Genomics 2007;8():385-385.</p><p>Published online 23 Oct 2007</p><p>PMCID:PMC2211320.</p><p></p>ifference in expression level between the -positive and the -negative ALL subclasses. SAM denotes Significant Analysis of Microarrays. Two groups of genes (according to the filters applied) were selected and functionally annotated. Nine of the 16 genes were selected for RT-PCR validation on the basis of their biological relevance

Five distinct biological processes and 14 differentially expressed genes characterize -positive leukemia-3

<p><b>Copyright information:</b></p><p>Taken from "Five distinct biological processes and 14 differentially expressed genes characterize -positive leukemia"</p><p>http://www.biomedcentral.com/1471-2164/8/385</p><p>BMC Genomics 2007;8():385-385.</p><p>Published online 23 Oct 2007</p><p>PMCID:PMC2211320.</p><p></p>ing the 14 selected genes for . Patients are segregated according to the presence or absence of the rearrangement. (B) Support tree of Set-A and Set-B patients using the 14 selected genes for . Two branches clearly distinguished -positive ALL and -negative ALL with 100% of reproducibility when resampling with replacement was conducted on experiments and genes for 100 iterations. The expression levels of the RUNX1 gene can explain the clustering of patients 9 and 17

Five distinct biological processes and 14 differentially expressed genes characterize -positive leukemia-2

<p><b>Copyright information:</b></p><p>Taken from "Five distinct biological processes and 14 differentially expressed genes characterize -positive leukemia"</p><p>http://www.biomedcentral.com/1471-2164/8/385</p><p>BMC Genomics 2007;8():385-385.</p><p>Published online 23 Oct 2007</p><p>PMCID:PMC2211320.</p><p></p>estalt pre-stored human genome gene set. Each circular area represents groups of genes, sharing common properties within relevant biological processes. Five discrete enriched GO categories are identified: cell differentiation, cell proliferation, apoptosis, cell motility and response to wounding. Enriched GO categories are represented by 16 annotated genes. Six (in bold) of these 16 genes had been previously identified, and two, in brackets, were not used for further analysis because of their tissue-specific expression patterns