9 research outputs found
Lysosomal exoglycosidases in serum and urine of patients with pancreatic adenocarcinoma.
Lysosomal exoglycosidases: N-acetyl-β-D-hexosaminidase (HEX), β-D-galactosidase (GAL), ι-L-fucosidase (FUC) and ι-D-mannosidase (MAN) modify oligosaccharide chains of glycoconjugates in endoplasmatic reticulum and/or Golgi apparatus and degrade them in lysosomes. In acid environment of lysosome, exoglycosidases degrade oligosaccharide chains of glycoproteins, glycolipids and glycosaminoglycans by eliminating single sugars from the edges of oligosaccharide chains. Neoplasms change biochemical processes in tissues and may significantly change the activity of many enzymes including the activity of lysosomal exoglycosidasses in serum and urine of persons with neoplasmatic diseases. The aim of the present paper was evaluation the activity of HEX, GAL, FUC and MAN in serum and urine of patients with pancreatic adenocarcinoma. Serum and urine samples were collected from 15 patients with adenocarcinoma of the pancreas and 15 healthy persons. The activity of lysosomal exoglycosidases was determined by the method of Marciniak et al. adapted to serum and urine of patients with adenocarcinoma of the pancreas. Our results indicate significant decrease in activity of GAL (p=0.037) in serum of patients with pancreatic adenocarcinoma, significant increase in activity of HEX (
Artykaina plus morfina versus sama artykaina w leczeniu bólu podczas zabiegu endodontycznego z powodu nieodwracalnego zapalenia miazgi : badanie randomizowane, kontrolowane
Standardowe znieczulenie miejscowe nie gwarantuje
całkowitego ustąpienia bólu podczas zabiegu endodontycznego
usuwania miazgi w stanie nieodwracalnego zapalenia.
Celem pracy było sprawdzenie, czy miejscowe znieczulenie
za pomocą morfiny w dawce 1 mg dodanej do standardowego
znieczulenia za pomocą artykainy w porównaniu z samą artykainą
zmniejszy odczuwanie bólu w czasie 48 godz. po zabiegu
endodontycznym. W podwójnie ślepej próbie klinicznej uczestniczyły
32 osoby, które miały nieodwracalne zapalenie miazgi
pierwszego zęba trzonowego szczęki. Uczestnicy badania byli
zrandomizowani do dwóch grup: grupy badawczej, która otrzymywała
artykainę i morfinę (n = 9), oraz grupy kontrolnej, która
otrzymywała tylko artykainę (n = 23). Nasilenie bólu badano
za pomocą numerycznej skali oceny bólu oraz skali wzrokowo-
-analogowej przed zabiegiem oraz 1, 2, 6, 12, 24 i 48 godz. po zabiegu. Nie stwierdzono istotnych różnic nasilenia bólu między
dwoma grupami w obu skalach oceny bólu we wszystkich kolejnych
punktach oceny aż do 48 godz. po zabiegu. Stwierdzono
jedynie nieistotne statystycznie mniejsze odczuwanie bólu po 12
godz. u chorych leczonych morfiną i artykainą (p = 0,08). Badanie
było finansowane z grantu Uniwersytetu Jagiellońskiego (UJ)
i miało zgodę Komisji Bioetycznej UJ nr: 2008/K/ZDS/000518. Dodanie morfiny do artykainy w celu mniejszego
odczuwania bólu podczas endodontycznego usuwania miazgi
w stanie nieodwracalnego zapalenia nie ma wpływu na odczuwanie
bólu, choć większą skuteczność tego znieczulenia obserwowano
wcześniej podczas zabiegów chirurgicznych w jamie ustnej.
Wskazane są dalsze badania oceniające skuteczność opioidów
w miejscowym znieczuleniu podczas zabiegów endodontycznych.Standard local anaesthetics do not guarantee
complete absence of pain during endodontic debridement of
the inflamed pulp.
We investigated whether the addition of l mg of morphine
to local articaine anaesthesia as compared to articaine alone
could reduce pain sensations during endodontic debridement
and within 48 h after the procedure. Thirty-two patients with pain attributed
to irreversible pulpitis of the first upper molar participated
in this randomized, double-blind trial. Patients were randomly
assigned either to the study group (patients receiving periapical
injection of modified local anaesthetic solution with morphine,
n = 9) or to the control group (patients receiving standard anaesthetic
solution without morphine, n = 23). The pain intensity
was assessed using the visual analogue scale and verbal score
before and directly after endodontic debridement, as well as at
1, 2, 6, 12, 24 and 48 h after the procedure. Neither the visual analogue scale nor verbal score differed
between both groups before or during the endodontic
debridement, as well as during 48-hour follow-up. However, there
was an insignificant tendency for greater pain relief in patients
treated with articaine and morphine at 12 h after endodontic
debridement (p = 0.08). The study was funded by a Jagiellonian
University in Poland (JU) grant, and was registered with the JU
Ethical Committee No. 2008/K/ZDS/000518. The addition of morphine to local articaine anaesthesia
does not influence pain intensity in patients undergoing
endodontic debridement in contrast to the previously described
pain-relief effect in patients after oral surgery. However, future
studies are needed to evaluate the role of opioids in local anaesthesia
in endodontic procedures
Lysosomal exoglycosidases in serum and urine of patients with pancreatic adenocarcinoma.
Molecular and cellular effects of a novel hydroxamate-based HDAC inhibitor – belinostat – in glioblastoma cell lines: a preliminary report
Trade in goods between the European Union and the United Kingdom after Brexit
Niniejsze opracowanie stanowi próbę przedstawienia rozwiązań regulujących wymianę towarową, przyjętych wobec opuszczenia Unii Europejskiej przez Zjednoczone Królestwo. Analizie zostały poddane w znacznej mierze postanowienia części pierwszej i drugiej Umowy o handlu i współpracy, jak również niektóre postanowienia Protokołu w sprawie Irlandii i Irlandii Północnej. W celu umożliwienia czytelnikom dokonania porównania pomiędzy aktualnym a przedbrexitowym stanem prawnym, w treści pracy przedstawiony został również skondensowany opis unijnej swobody przepływu towarów.The aim of the presented thesis is to demonstrate established solutions which regulate trade in goods and were adopted after the United Kingdom left the European Union. The provisions of Part One and Part Two of the Trade and Cooperation Agreement were the main subject to analysis, as well as some provisions of the Northern Ireland Protocol. In order to enable readers to make a comparison between the current and pre-Brexit legal status, the content of the thesis includes a condensed description of EU free movement of goods
Low-glucose medium induces ORP150 expression and exerts inhibitory effect on apoptosis and senescence of human breast
MCF7 cell
A Preliminary Study of the Effect of Quercetin on Cytotoxicity, Apoptosis, and Stress Responses in Glioblastoma Cell Lines
A growing body of evidence indicates that dietary polyphenols show protective effects against various cancers. However, little is known yet about their activity in brain tumors. Here we investigated the interaction of dietary flavonoid quercetin (QCT) with the human glioblastoma A172 and LBC3 cell lines. We demonstrated that QCT evoked cytotoxic effect in both tested cell lines. Microscopic observations, Annexin V-FITC/PI staining, and elevated expression and activity of caspase 3/7 showed that QCT caused predominantly apoptotic death of A172 cells. Further analyses confirmed enhanced ROS generation, deregulated expression of SOD1 and SOD2, depletion of ATP levels, and an overexpression of CHOP, suggesting the activation of oxidative stress and ER stress upon QCT exposure. Finally, elevated expression and activity of caspase 9, indicative of a mitochondrial pathway of apoptosis, was detected. Conversely, in LBC3 cells the pro-apoptotic effect was observed only after 24 h incubation with QCT, and a shift towards necrotic cell death was observed after 48 h of treatment. Altogether, our data indicate that exposure to QCT evoked cell death via activation of intrinsic pathway of apoptosis in A172 cells. These findings suggest that QCT is worth further investigation as a potential pharmacological agent in therapy of brain tumors
Lysosomal exoglycosidases in serum and urine of patients with pancreatic adenocarcinoma.
Lysosomal exoglycosidases: N-acetyl-β-D-hexosaminidase (HEX), β-D-galactosidase (GAL), ι-L-fucosidase (FUC) and ι-D-mannosidase (MAN) modify oligosaccharide chains of glycoconjugates in endoplasmatic reticulum and/or Golgi apparatus and degrade them in lysosomes. In acid environment of lysosome, exoglycosidases degrade oligosaccharide chains of glycoproteins, glycolipids and glycosaminoglycans by eliminating single sugars from the edges of oligosaccharide chains. Neoplasms change biochemical processes in tissues and may significantly change the activity of many enzymes including the activity of lysosomal exoglycosidasses in serum and urine of persons with neoplasmatic diseases. The aim of the present paper was evaluation the activity of HEX, GAL, FUC and MAN in serum and urine of patients with pancreatic adenocarcinoma. Serum and urine samples were collected from 15 patients with adenocarcinoma of the pancreas and 15 healthy persons. The activity of lysosomal exoglycosidases was determined by the method of Marciniak et al. adapted to serum and urine of patients with adenocarcinoma of the pancreas. Our results indicate significant decrease in activity of GAL (p=0.037) in serum of patients with pancreatic adenocarcinoma, significant increase in activity of HEX (p<0.001) and FUC (p=0.027) in serum, and HEX (p=0.003) in urine, as well as significant decrease of FUC (p=0.016) and MAN (p=0.029) in urine o patients with adenocarcinoma of the pancreas, in comparison to the control group. Increase in activity of some lysosomal enzymes in serum and urine of pancreatic adenocarcinoma patients, may indicate on destruction of pancreatic tissue by pancreatic adenocarcinoma. Determination of the HEX, GAL, FUC and MAN in serum and urine may be useful in diagnostics of pancreatic adenocarcinoma