The Impacts of Megahydraulic Engineering. Projects from a Dutch Perspective

Differentially expressed genes in response to the fucoxanthin treatment. (XLSX 15 kb

Influence of low doses of γ-irradiation on the lifespan of <i>Drosophila melanogaster</i>, wild-type line <i>Canton-S</i>.

<p>A–males, B–females, *—p<0.05, **—p<0.01, (Kolmogorov-Smirnov test).</p

Strehler-Mildvan correlation between the parameters of the Gompertz function in <i>Drosophila melanogaster</i> wild-type <i>Canton-S</i> individuals exposed to low doses of ionizing radiation.

<p>Strehler-Mildvan correlation between the parameters of the Gompertz function in <i>Drosophila melanogaster</i> wild-type <i>Canton-S</i> individuals exposed to low doses of ionizing radiation.</p

The genes selected for expression analysis in the samples of <i>Drosophila melanogaster</i> wild-type strain <i>Canton-S</i> 72 hours after radiation exposure in doses from 5 cGy to 40 cGy.

<p>The genes selected for expression analysis in the samples of <i>Drosophila melanogaster</i> wild-type strain <i>Canton-S</i> 72 hours after radiation exposure in doses from 5 cGy to 40 cGy.</p

The differentially expressed genes in <i>Drosophila melanogaster</i> males and females after the radiation exposure.

<p>A– 5 cGy, B– 10 cGy, C– 20 cGy, D– 40 cGy, 1 –males, 2 –females. Only gene changes with Log₂FC > 1 and p-value < 0.05 during at least one time range are presented.</p

Analysis of the gene expression by the qPCR in the samples of <i>Drosophila melanogaster</i> wild-type strain <i>Canton-S</i> 72 hours after radiation exposure in doses from 5 cGy to 40 cGy (Female/male).

<p>n–FC absolute value < 2; ǀLog₂FCǀ<1</p><p>+–Log₂FC > 1</p><p>-–Log₂FC < -1</p><p>*—p-value < 0.05</p><p>Analysis of the gene expression by the qPCR in the samples of <i>Drosophila melanogaster</i> wild-type strain <i>Canton-S</i> 72 hours after radiation exposure in doses from 5 cGy to 40 cGy (Female/male).</p

Effect of Low Doses (5-40 cGy) of Gamma-irradiation on Lifespan and Stress-related Genes Expression Profile in <i>Drosophila melanogaster</i>

<div><p>Studying of the effects of low doses of γ-irradiation is a crucial issue in different areas of interest, from environmental safety and industrial monitoring to aerospace and medicine. The goal of this work is to identify changes of lifespan and expression stress-sensitive genes in <i>Drosophila melanogaster</i>, exposed to low doses of γ-irradiation (5 – 40 cGy) on the imaginal stage of development. Although some changes in life extensity in males were identified (the effect of hormesis after the exposure to 5, 10 and 40 cGy) as well as in females (the effect of hormesis after the exposure to 5 and 40 cGy), they were not caused by the organism “physiological” changes. This means that the observed changes in life expectancy are not related to the changes of organism physiological functions after the exposure to low doses of ionizing radiation. The identified changes in gene expression are not dose-dependent, there is not any proportionality between dose and its impact on expression. These results reflect nonlinear effects of low dose radiation and sex-specific radio-resistance of the postmitotic cell state of <i>Drosophila melanogaster </i>imago.</p></div

Additional file 1: of Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.)

Genes for which expression was specifically changed in resistant genotypes of flax in response to Fusarium oxysporum infection. (XLSX 9584 kb

Additional file 2: of Differential gene expression in response to Fusarium oxysporum infection in resistant and susceptible genotypes of flax (Linum usitatissimum L.)

Expression profiles of genes for selected gene ontology (GO) terms in susceptible and resistant flax genotypes in response to Fusarium oxysporum infection. (XLSX 861 kb

List of unique genes which are up- and down-regulated in both sexes after a particular treatment.

*<p>Gene names were obtained from <a href="http://flybase.org" target="_blank">http://flybase.org</a>.</p>**<p><a href="http://www.uniprot.org" target="_blank">http://www.uniprot.org</a>.</p>***<p>According to Gene Expression Atlas, <a href="http://www.ebi.ac.uk/gxa" target="_blank">http://www.ebi.ac.uk/gxa</a>.</p