Enantioselective Carbon Stable Isotope Fractionation of Hexachlorocyclohexane during Aerobic Biodegradation by <i>Sphingobium</i> spp.

Carbon isotope fractionation was investigated for the biotransformation of γ- and α- hexachlorocyclohexane (HCH) as well as enantiomers of α-HCH using two aerobic bacterial strains: <i>Sphingobium indicum</i> strain B90A and <i>Sphingobium japonicum</i> strain UT26. Carbon isotope enrichment factors (ε_c) for γ-HCH (ε_c = −1.5 ± 0.1‰ and −1.7 ± 0.2‰) and α-HCH (ε_c = −1.0 ± 0.2‰ and −1.6 ± 0.3‰) were similar for both aerobic strains, but lower in comparison with previously reported values for anaerobic γ- and α-HCH degradation. Isotope fractionation of α-HCH enantiomers was higher for (+) α-HCH (ε_c = −2.4 ± 0.8 ‰ and −3.3 ± 0.8 ‰) in comparison to (−) α-HCH (ε_c = −0.7 ± 0.2‰ and −1.0 ± 0.6‰). The microbial fractionation between the α-HCH enantiomers was quantified by the Rayleigh equation and enantiomeric fractionation factors (ε_e) for <i>S. indicum</i> strain B90A and <i>S. japonicum</i> strain UT26 were −42 ± 16% and −22 ± 6%, respectively. The extent and range of isomer and enantiomeric carbon isotope fractionation of HCHs with <i>Sphingobium</i> spp. suggests that aerobic biodegradation of HCHs can be monitored in situ by compound-specific stable isotope analysis (CSIA) and enantiomer-specific isotope analysis (ESIA). In addition, enantiomeric fractionation has the potential as a complementary approach to CSIA and ESIA for assessing the biodegradation of α-HCH at contaminated field sites

Carbon, Hydrogen and Chlorine Stable Isotope Fingerprinting for Forensic Investigations of Hexachlorocyclohexanes

Multielemental stable isotope analysis of persistent organic pollutants (POPs) has the potential to characterize sources, sinks, and degradation processes in the environment. To verify the applicability of this approach for source identification of hexachlorocyclohexane (HCHs), we provide a data set of carbon, hydrogen, and chlorine stable isotope ratios (δ¹³C, δ²H, δ³⁷Cl) of its main stereoisomers (α-, β-, δ- and γ-HCHs) from a sample collection based on worldwide manufacturing. This sample collection comprises production stocks, agricultural and pharmaceutical products, chemical waste dumps, and analytical-grade material, covering the production time period from the late 1960s until now. Stable isotope ratios of HCHs cover the ranges from −233‰ to +1‰, from −35.9‰ to −22.7‰, and from −6.69‰ to +0.54‰ for δ²H, δ¹³C, and δ³⁷Cl values, respectively. Four groups of samples with distinct multielemental stable isotope fingerprints were differentiated, most probably as a result of purification and isolation processes. No clear temporal trend in the isotope compositions of HCHs was found at the global scale. The multielemental stable isotope fingerprints facilitate the source identification of HCHs at the regional scale and can be used to assess transformation processes. The data set and methodology reported herein provide basic information for the assessment of environmental field sites contaminated with HCHs