EKSPRESI PROTEIN COAT DAN mRNA VIRAL NERVOUS NECROSIS YANG DIKENDALIKAN OLEH PROMOTER β-AKTIN IKAN MEDAKA DAN KERATIN IKAN FLOUNDER JEPANG

Kemampuan promoter dalam mengatur ekspresi gen penyandi protein imunogenik sangat menentukan efikasi suatu vaksin DNA. Penelitian ini bertujuan untuk mengukur tingkat ekspresi protein dan mRNA RNA2 penyandi coat protein (CP) virus viral nervous necrosis (VNN) yang dikendalikan oleh dua promoter berbeda, yaitu promoter β-aktin ikan medaka (mBA), dan keratin ikan flounder Jepang (JfKer). Uji ekspresi CP dilakukan menggunakan embrio ikan lele dumbo (Clarias sp.) sebagai model, sedangkan analisis mRNA dilakukan menggunakan ikan kerapu tikus. Konstruksi vektor ekspresi pmBA-CP dan pJKer-CP dengan konsentrasi 50 ng/μL KCl 1 M disuntikkan ke embrio ikan lele dumbo fase 1-2 sel. Sebanyak 30 embrio ikan lele dumbo diambil pada jam ke-6, 8, 10, 12, 14, dan 16 pascainjeksi untuk analisis protein. Hasil SDS-PAGE menunjukkan adanya protein berukuran sekitar 42 kDa, dan analisis western blot menggunakan antibodi (Ab) poliklonal anti-VNN membuktikan bahwa protein tersebut adalah CP. Keberhasilan deteksi protein spesifik menggunakan Ab anti-VNN tersebut menunjukkan bahwa embrio ikan lele dapat digunakan untuk menguji potensi produksi protein imunogenik yang dikendalikan oleh promoter berbeda. Pengujian ini juga menunjukkan bahwa, aktivitas promoter mBA lebih tinggi daripada promoter JfKer, sehingga uji ekspresi mRNA dilakukan menggunakan konstruksi pmBA-CP. Benih ikan kerapu tikus (panjang badan sekitar 5 cm) diinjeksi dengan pmBA-CP secara intramuskular dengan dosis 12,5 μg/ekor. Total RNA diekstraksi dari daging pada waktu 6, 12, dan 24 jam pascainjeksi. Hasil RT-PCR menunjukkan adanya ekspresi mRNA CP pada 24 jam pascainjeksi. Hal tersebut menunjukkan bahwa promotor mBA aktif mengendalikan ekspresi CP pada ikan kerapu tikus, dan pmBA-CP berpotensi digunakan sebagai vaksin DNA untuk menginduksi kekebalan ikan kerapu terhadap infeksi VNN

Pemanfaatan Rumput Laut Latoh (Caulerpa sp.) Sebagai Snack Sehat Pencegah Stunting di Desa Bulu, Kecamatan Jepara, Kabupaten Jepara

Stunting is a condition of malnutrition associated with chronic nutritional insufficiency. The stunting rate in Central Java Province was recorded at 20.9%. Then in 2022 it will drop to 20.8%. However, this figure still needs to be reduced to the lowest number. According to UNICEF Indonesia, the cause of stunting is the practice of parenting and feeding children who are inadequate, causing high rates of malnutrition. The nutritional intake needed by toddlers and pregnant women can be in the form of folic acid, vitamins, minerals, iron, calcium, fiber to protein. One of the foodstuffs that have a high nutritional content is latoh. This service aims to provide counseling or education about the importance of nutrition in children to prevent stunting. In addition, it can educate the public about the processing techniques of Latoh seaweed as a healthy snack. This service activity method begins with preparation, delivery of material, and trying to make processed products made from latoh together. Results from the activities carried out there were 50% of respondents who consumed latoh on counseling days. In addition, nutrition is important for children by consuming healthy latoh preparations. Then the presenter also conveyed the technique of processing Latoh seaweed into a healthy snack.Stunting is a condition of malnutrition associated with chronic nutritional insufficiency. The stunting rate in Central Java Province was recorded at 20.9%. Then in 2022 it will drop to 20.8%. However, this figure still needs to be reduced to the lowest number. According to UNICEF Indonesia, the cause of stunting is the practice of parenting and feeding children who are inadequate, causing high rates of malnutrition. The nutritional intake needed by toddlers and pregnant women can be in the form of folic acid, vitamins, minerals, iron, calcium, fiber to protein. One of the foodstuffs that have a high nutritional content is latoh. This service aims to provide counseling or education about the importance of nutrition in children to prevent stunting. In addition, it can educate the public about the processing techniques of Latoh seaweed as a healthy snack. This service activity method begins with preparation, delivery of material, and trying to make processed products made from latoh together. Results from the activities carried out there were 50% of respondents who consumed latoh on counseling days. In addition, nutrition is important for children by consuming healthy latoh preparations. Then the presenter also conveyed the technique of processing Latoh seaweed into a healthy snack