Pentakis-thiacalix[4]arenes with nitrile fragments: Receptor properties toward cations of some s-and d-metals and self-assembly of nanoscale aggregates

Pentakis-thiacalix[4]arenes containing thiacalix[4]arene fragments in cone, partial cone and 1,3-alternate conformations as their central core have been obtained. Their interaction with cations of s- and d-metals gives supramolecular assemblies whose composition depends on the conformation of pentakis-thiacalix[4]arenes and the nature of the 'guests'. © 2013 Mendeleev Communications. All rights reserved

Enantioselective recognition of amino acids by enantiomerically pure calix[4]arene carboxylic acid or their diastereomerically pure N-(1-phenyl)ethyl amides

The interaction of inherently chiral calix[4]arene carboxilic acids and their amides with amino acids in the organic phase has been studied using electron spectroscopy. It was found that the chiral calix[4]arenes are able of enantioselective recognition of L- and D-forms of amino acids. Stability constants of the calixarene - amino acid supramolecular complexes were determined and mechanism of the host-guest interaction was examined by molecular modeling method. © ISUCT Publishing

Comparative DNA Cytometry of Primary and Recurrent Soft Tissue Sarcomas

The goal of comparative investigation was to reveal the distinctive features of the DNA content and cell distribution in the phases of the cell cycle of recurrent STS. DNA cytometry in the tumor tissue of 30 primary soft tissue sarcomas (t2a-2bn0M0) and 30 STS recurrences (t2-3n0M1) was carried out using the method of flow cytofluorometry. the tumor ploidy and cell distribution in the cell cycle phases were analyzed. Results. A number of differences in the DNA cytometric parameters of primary and recurrent STS have been revealed, they include: an increase in the proportion of aneuploid tumors in case of recurrence, the number of tumors with DNA index within the mitotic cycle, an increase in the proportion of cells in G2+M- phase of diploid and aneuploidy tumors and a decrease in S- phase of aneuploid ones. It has been shown that with a G2 differentiation degree, the proportion of cells in G2+M, S- and IP of recurrent STS is significantly lower than the primary parameters. An increase in the proportion of cells in G2+M- phase and a decrease in the rate of proliferation of recurrent STS, depending on the stage, are shown only in case of stage III. Conclusion. The revealed features of DNA content and cell cycle of tumor cells of soft tissue sarcomas will allow to approach to understanding of biological bases of recurrence of this malignant disease.Целью исследования было выявить в сравнительном аспекте отличительные особенности содержания ДНК и распределения клеток по фазам клеточного цикла рецидивных СМТ. Методом проточной цитофлуориметрии проводили ДНК-цитометрию в опухолевой ткани 30 первичных сарком мягких тканей (T2a-2bN0M0) и 30 – рецидивов СМТ (t2-3n0M1). анализировали плоидность опухоли и распределение клеток по фазам клеточного цикла. результаты. Выявлен ряд различий ДНК-цитометрических показателей первичных и рецидивных СМТ, которые заключаются: в увеличении доли анеуплоидных опухолей при рецидивах, числа опухолей с ИДНК в пределах митотического цикла, увеличение доли клеток в G2+M- фазе диплоидных и анеуплоидных опухолей и снижение в S-фазе анеуплоидных. Показано, что при степени дифференцировки G2 доля клеток в фазах G2+M, S- и ИП рецидивных СМТ значимо ниже параметров первичных. Увеличение доли клеток в G2+M-фазе и снижение темпов пролиферации рецидивных СМТ в зависимости от стадии показаны только при III стадии. Выводы. Выявленные особенности содержания ДНК и параметров клеточного цикла опухолевых клеток сарком мягких тканей позволят приблизиться к пониманию биологических основ рецидивирования этого злокачественного заболевания

Pentakis-thiacalix[4]arenes with nitrile fragments: Receptor properties toward cations of some s-and d-metals and self-assembly of nanoscale aggregates

Pentakis-thiacalix[4]arenes containing thiacalix[4]arene fragments in cone, partial cone and 1,3-alternate conformations as their central core have been obtained. Their interaction with cations of s- and d-metals gives supramolecular assemblies whose composition depends on the conformation of pentakis-thiacalix[4]arenes and the nature of the 'guests'. © 2013 Mendeleev Communications. All rights reserved

Pentakis-thiacalix[4]arenes with nitrile fragments: Receptor properties toward cations of some s-and d-metals and self-assembly of nanoscale aggregates

Pentakis-thiacalix[4]arenes containing thiacalix[4]arene fragments in cone, partial cone and 1,3-alternate conformations as their central core have been obtained. Their interaction with cations of s- and d-metals gives supramolecular assemblies whose composition depends on the conformation of pentakis-thiacalix[4]arenes and the nature of the 'guests'. © 2013 Mendeleev Communications. All rights reserved

Enantioselective recognition of amino acids by enantiomerically pure calix[4]arene carboxylic acid or their diastereomerically pure N-(1-phenyl)ethyl amides

The interaction of inherently chiral calix[4]arene carboxilic acids and their amides with amino acids in the organic phase has been studied using electron spectroscopy. It was found that the chiral calix[4]arenes are able of enantioselective recognition of L- and D-forms of amino acids. Stability constants of the calixarene - amino acid supramolecular complexes were determined and mechanism of the host-guest interaction was examined by molecular modeling method. © ISUCT Publishing

Enantioselective recognition of amino acids by enantiomerically pure calix[4]arene carboxylic acid or their diastereomerically pure N-(1-phenyl)ethyl amides

The interaction of inherently chiral calix[4]arene carboxilic acids and their amides with amino acids in the organic phase has been studied using electron spectroscopy. It was found that the chiral calix[4]arenes are able of enantioselective recognition of L- and D-forms of amino acids. Stability constants of the calixarene - amino acid supramolecular complexes were determined and mechanism of the host-guest interaction was examined by molecular modeling method. © ISUCT Publishing

Enantioselective recognition of amino acids by enantiomerically pure calix[4]arene carboxylic acid or their diastereomerically pure N-(1-phenyl)ethyl amides

The interaction of inherently chiral calix[4]arene carboxilic acids and their amides with amino acids in the organic phase has been studied using electron spectroscopy. It was found that the chiral calix[4]arenes are able of enantioselective recognition of L- and D-forms of amino acids. Stability constants of the calixarene - amino acid supramolecular complexes were determined and mechanism of the host-guest interaction was examined by molecular modeling method. © ISUCT Publishing

DNA-cytometric characteristics of recurrent soft tissue sarcomas

Aim. To determine the content of deoxyribonucleic acid (DNA) and distribution of cells in mitotic phases in patients with recurrent soft tissue sarcomas for the assessment of malignancy of the process. Methods. Tumor tissues of patients with recurrent soft tissue sarcomas were studied. Research methods included histological, DNA-cytometric and statistical methods. Results. Proliferative activity and proliferative index of recurrent sarcomas differed depending on the tumor grade and stage. Differences in the number of diploid, aneuploid and polyploid cells were determined in each group and between the groups depending on the cell cycle phases. Cell cycle parameters were as following: 100% of G1 (well-differentiated) cancer were diploid, as well as 33.3% of G2 (moderately differentiated) and 15% of G3 (poorly differentiated) tumors. Aneuploid tumors prevailed in G2 and G3, the ratio of which was 66.7 and 85%, respectively. The analysis of kinetic parameters of the cell cycle allowed establishing a decrease in the number of cells in G1/G0 phase of the cell cycle from G1 to G3, which was accompanied by a statistically significant increase in the proportion of cells in S-phase (p ˂0.05). Conclusion. The DNA-cytometric study of cell cycle parameters showed high biological potential of recurrent soft tissue sarcomas, which was determined by two indices - the proportion of cells in G2+M-phase and the cell loss factor; 100% of well-differentiated (G1) tumors, 33.3% of moderately differentiated (G2) and 15% of poorly differentiated (G3) tumors were diploid; aneuploid tumors prevailed in G2 and G3