Expression profile of insulin 1 and insulin 2 (panels A and B, respectively) in the whole pancreas of cort+/+ and cort−/− male mice fed a low fat (LF) or high fat diet (HFD).

<p>C, plasma insulin level obtained from cort+/+ and cort−/− animals fed a LF- or HF-diet. Values represent mean ± SEM of copy number adjusted by normalization factor (NF) per 100 ng of cDNA obtained from 4–7 animals per experimental group. (*: p<0,05).</p

Evaluation of in vitro CORT action on glucose induced-Insulin release in cultured pancreatic islets from cort−/− mice.

<p>Values represent mean ± SEM obtained from 3 independent experiments. Different letters stand for statistical differences (p<0,05).</p

Expression level of different ghrelin system components in the whole pancreas of cort+/+ and cort−/− male mice fed a low fat (LF) or high fat diet (HFD).

<p>Values represent mean ± SEM of copy number adjusted by normalization factor (NF) per 100 ng of cDNA obtained from 3–7 animals per experimental group. (*: p<0,05; **: p<0,01; ***: p<0,001).</p

Expression profile of Insulin 1 and insulin 2 in pancreatic islets of cort+/+ and cort−/− male mice fed a low fat (LF) or high fat diet (HFD).

<p>Values represent mean ± SEM of copy number adjusted by β-actin expression per 100 ng of cDNA obtained from 2–3 animals per experimental group. (*: p<0,05).</p

Expression level of different ghrelin system components in pancreatic islets of cort+/+ and cort−/− male mice fed a low fat (LF) or high fat diet (HFD).

<p>Values represent mean ± SEM of copy number adjusted by normalization factor (NF) per 100 ng of cDNA obtained from 4–7 animals per experimental group. (*:p<0,05; **: p<0,01; ***: p<0,001).</p

Specific set of primers for amplification of mouse transcripts used for quantitative real-time RT-PCR.

<p><i>Bp</i> base pairs; <i>Sn</i> sense; <i>As</i> antisense.</p><p>Specific set of primers for amplification of mouse transcripts used for quantitative real-time RT-PCR.</p

Impact of diet-induced obesity on the expression of ghrelin system in the mammary fat pads of CORT-KO, SST-KO and their respective control mice.

<p>mRNA levels of ghrelin, In2-ghrelin, GOAT were measured by qPCR. Values represent means ± SEM of the mRNA copy number of each transcript adjusted by the normalization factor (n = 5–6). Global differences by 2-way ANOVA are shown at the top of each graphic (G: genotype effect; D: diet effect I: effect of interaction between genotype and diet; *, p<0.05).</p

Impact of diet-induced obesity on the expression of SST/CORT/receptor subtypes in the mammary fat pads of CORT-KO, SST-KO and their respective control mice.

<p>mRNA levels of sst1, sst2, sst3, sst4 were measured by qPCR. Values represent means ± SEM of the mRNA copy number of each transcript adjusted by the normalization factor (n = 5–6). Global differences by 2-way ANOVA are shown at the top of each graphic (G: genotype effect; D: diet effect I: effect of interaction between genotype and diet; *, p<0.05; **, p<0.01; ***, p<0.001). Asterisks above the bars (*, p<0.05; **, p<0.01) indicate significant differences between groups by Bonferroni post-hoc.</p

Characterization of GH/IGF-I axis and the regulatory SST/CORT/ghrelin/receptors system in mammary fat pads of female mice (expressed as absolute copy number/50 ng total cDNA).

<p>Values represent means ± SEM of the mRNA copy number of each transcript (n = 5–6 mice).</p

Plasma leptin levels in WT, CORT-KO and SST-KO mice fed under LF and HF diets.

<p>Values represent mean ± SEM of ng/ml of plasma leptin. Global differences by 2-way ANOVA are shown at the top of each graphic (G: genotype effect; D: diet effect I: effect of interaction between genotype and diet; *, p<0.05; **, p<0.01; ***, p<0.001). Asterisks above the bars (*, p<0.05) indicate significant differences between groups by Bonferroni post-hoc.</p