MOESM2 of Bacterial polyextremotolerant bioemulsifiers from arid soils improve water retention capacity and humidity uptake in sandy soil

Additional file 2: Figure S1. Phylogenetic affiliation of partial 16S rRNA gene of the 23 bacterial isolates obtained from chott and desert in the south of Tunisia constructed using MEGA6 package. Neighbor-Joining phylogenetic tree was built using MEGA 6, computing the evolutionary distances using the Kimura 2-parameter model

MOESM1 of Bacterial polyextremotolerant bioemulsifiers from arid soils improve water retention capacity and humidity uptake in sandy soil

Additional file 1:Table S1. List of the 23 bacterial strains used in this work

Expression of 7 immune genes in <i>Ceratitis capitata</i> inoculated with <i>P</i>. <i>lilacinum</i> for the times analyzed (0h, 24h, 72h, and 144h).

The bottom and top “whiskers” represent minimum and maximum values, respectively. The thick line bisecting the box represents the median. The bottom and top of the box represent the 25th and 75th percentiles, respectively. Asterisks indicate a significant difference (* pS1 Data.</p

Digestive glycosyl hydrolase activities after fungal inoculation to <i>Ceratitis capitata</i> (24 to 144 hours post-inoculation).

Blue and red bars chart represent glycosyl hydrolase activities mg protein for non-infected and infected flies respectively. (A) β –galactosidase activities; (B) α-glucosidase activities; (C) α-galactosidase activities and (D) β–glucosidase activities. Substrates used: PNPaglu, PNPbglu, PNPagal, PNPbgal. Error bars indicate the standard error. (* p<0.05, ** p<0.01, *** p<0.001).</p

Average amounts of lipid, glycogen, and sugar in infected larvae (red) and non-infected larvae (blue) of <i>Ceratitis capitata</i>.

Error bars indicate the standard error. (*) indicates a significant difference between columns (P<0.05).</p

Digestive proteinase activities after fungal inoculation to <i>Ceratitis capitata</i> (24 to 144 hours post-inoculation).

(A) Blue bar charts represent azocaseinolytic activities per mg of protein for non-infected flies; red bar charts represent azocaseinolytic activities per mg of protein for infected flies. (B) Blue bar charts represent hemoglobinasic activities per mg of protein for non-infected flies; red bar charts represent hemoglobinasic activities per mg of protein for infected flies. Error bars indicate the standard error. (* p<0.05, ** p<0.01, *** p<0.001).</p

Survival analysis of <i>Ceratitis capitata</i> males and females, infected and non-infected (controls) with <i>P</i>. <i>lilacinum</i>.

Survival analysis of Ceratitis capitata males and females, infected and non-infected (controls) with P. lilacinum.</p

Variance Analysis tables of the complete linear models for the 7 genes of the study.

Variance Analysis tables of the complete linear models for the 7 genes of the study.</p

Digestive disaccharide and polysaccharide activities after fungal inoculation to <i>Ceratitis capitata</i> (24 to 144 hours post-inoculation).

Blue and red bars chart illustrating enzymatic activities per mg protein for non-infected and infected flies respectively. (A) represent enzymatic activities on starch; (B) represent enzymatic activities on maltose (C) represent enzymatic activities on sucrose; (D) represent enzymatic activities on pectin. Error bars indicate the standard error. (* p<0.05, ** p<0.01, *** p<0.001).</p

Effect of <i>P</i>. <i>lilacinum</i> on fecundity and fertility of <i>Ceratits capitata</i>.

(A) Bar chart illustrating the mean number of eggs laid by non-infected and infected females of C. capitata; (B) Bar chart showing the mean proportion of eggs that hatched from eggs laid by non-infected and infected females of C. capitata. Error bars indicate the standard error. (*) indicates a significant difference between infected adults and non-infected (p<0.05).</p