3 research outputs found
<i>In situ Carica papaya</i> stem matrix and <i>Fusarium oxysporum</i> (NCBT-156) mediated bioremediation of chromium
925-931Removal
of heavy metal chromium was carried out using the fungus Fusarium oxysporum NCBT-156 strain isolated from soil of leather
tanning effluent in in situ condition
using potassium dichromate solution with 10 per cent Czapek-dox liquid medium.
Biosorbent matrix was developed using Carica papaya plant dry stem to
colonize the fungal strain to facilitate bioabsorption process. Bioabsorption
of chromium was by metabolically mediated intracellular accumulation process.
Maximum efficiency of chromium removal by biosorption upto 90 per cent was
achieved at the end of 5th day of incubation (120 h of contact time) for 100
and 200 ppm concentration, upto 80 per cent for 300 and 400 ppm, and upto 65
per cent for 500 ppm to 1000 ppm concentrations with pH ranging from
5.8, 5.6, 5.5, 5.4 and 5.2, respectively for 100, 200, 300, 400, 500-1000 ppm
concentration. SDS-PAGE protein profile showed significant difference in 34 kDa
protein band after chromium absorption by the fungus. ftir spectroscopic analysis revealed that the main functional
groups involved in the uptake of chromium by F. oxysporium strain
were carbonyl, carboxyl, amino and hydroxyl groups