Development And Characterization Of 15 Microsatellite Loci For Cariniana Estrellensis And Transferability To Cariniana Legalis, Two Endangered Tropical Tree Species

From a genomic library enriched for GA/CA repeats, 15 highly polymorphic microsatellite markers were developed for Cariniana estrellensis, a tropical forest tree. The microsatellite loci were screened in 49 mature trees found between Pardo river and Mogi-Guaçu river basins, in the state of São Paulo, Brazil. A total of 140 alleles were detected with an average of 9.33 alleles per locus. The expected heterozygosity ranged from 0.37 to 0.88. These loci showed a high probability of paternity exclusion. Additionally, 12 loci were effectively transferred to Cariniana legalis. High levels of polymorphism make the present SSR markers useful for population genetic studies. © 2008 Springer Science+Business Media B.V.10410011004Billotte, N., Lagoda, P.J.L., Risterucci, A.M., Baurens, F.C., Microsatellite-enriched libraries: Applied methodology for the development of SSR markers in tropical crops (1999) Fruits, 54, pp. 277-288Doyle, J.J., Doyle, J.L., Isolation of plant DNA from fresh tissue (1990) Focus, 12, pp. 13-15Ferreira-Ramos, R., Laborda, P.R., Santos, M.O., Mayor, M.S., Mestriner, M.A., Souza, A.P., Alzate-Marin, A.L., Genetic analysis of forest species Eugenia uniflora L. through of newly developed SSR markers (2007) Conserv Genet., , doi: 10.1007/s10592-007-9458-0Goudet, J., (2002) FSTAT Version 2.9.3.2, , http://www2.unil.ch/popgen/softwares/fstat.htm, Institute of Ecology, Lausanne, Switzerland Accessed 20 May 2008Kalinowski, S.T., Taper, M.L., Marshall, T.C., Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment (2007) Mol Ecol, 16, pp. 1099-1106. , 10.1111/j.1365-294X.2007.03089.xLeite, E.J., State-of-knowledge on Cariniana estrellensis (Raddi) Kuntze (Lecythidaceae) for genetic conservation in Brazil (2007) Res J Bot, 2, pp. 138-160Rozen, S., Skaletsky, H.J., Primer3 on the www for general users and for biologist programmers (2000) Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365-386. , http://www.frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi, Krawetz S, Misener S (eds) Humana Press, Totowa Accessed 05 June 200