Ataf Ali - Excell Data from Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

DNA sensing always has an open meadow of curiosity for biotechnologists and other researchers. Recently, in this field, we have introduced an emerging class of molecules containing azo and guanidine functionalities. In this study, we have synthesized three new compounds (<b>UA1</b>, <b>UA6</b> and <b>UA7</b>) for potential application in DNA sensing in alcoholic medium. The synthesized materials were characterized by elemental analysis, FTIR, UV-visible, ¹H NMR and ¹³C NMR spectroscopies. Their DNA sensing potential were investigated by UV-visible spectroscopy. The insight of interaction with DNA was further investigated by electrochemical (cyclic voltammetry) and hydrodynamic (viscosity) studies. The results showed that compounds have moderate DNA binding properties, with the binding constants range being 7.2 x 10³, 2.4 x 10³ and 0.2 x 10³M^-1, for <b>UA1</b>, <b>UA6</b> and <b>UA7</b>, respectively. Upon binding with DNA, there was a change in colour (a blue shift in the <i>λ</i>_max value) which was observable with a naked eye. These results indicated the potential of synthesized compounds as DNA sensors with detection limit 1.8, 5.8 and 4.0ng µl^-1 for <b>UA1</b>, <b>UA6</b> and <b>UA7</b>, respectively

Supplementary Figure 1 from Synthesis and characterization of azo-guanidine based alcoholic media naked eye DNA sensor

UV-Visible spectrum of compound of UA6 (50µM) without DNA and with DNA interaction (20µM-140µM) in 80% ethanol, phosphate buffer (0.1M, pH=7.0). The full scan from 200nm to 800nm