PCR amplification results of 37 microsatellites markers tested on Scombridae species (<i>Thunnus albacares</i>, <i>Thunnus thynnus</i>, <i>Thunnus obesus</i>, <i>Acanthocybium solandri</i>) with 4 or 5 individuals per species.

<p>Weak amplified product in bold. Very weak amplified product in bold and italic. Smear in grey.</p><p>PCR amplification results of 37 microsatellites markers tested on Scombridae species (<i>Thunnus albacares</i>, <i>Thunnus thynnus</i>, <i>Thunnus obesus</i>, <i>Acanthocybium solandri</i>) with 4 or 5 individuals per species.</p

Microsatellite markers developed for Thunnus alalunga (43) with the corresponding GenBank number.

<p>Sequence range size in base pairs (complete sequence—primers, microsatellites and flanking region), primers sequence, number of repeats in the microsatellite motif determinate, microsatellite sequence corresponding at >75% alignment from GenBank NR and BOLD sequences, summary on the «non-neutral» gene alignment details, and information on the class of the markers («neutral», «non-neutral» and selected or not in the final panel).</p><p>Microsatellite markers developed for Thunnus alalunga (43) with the corresponding GenBank number.</p

Summary statistics of the potentially selective «neutral» microsatellite markers (34) for albacore (<i>Thunnus alalunga</i>).

<p>Number of individuals (Nind). Number of alleles (A). Expected (He), unbiased Nei's (1978) expected (H.n.b) and observed (HO) heterozygosity. Polymorphism information content (PIC). Null allele frequency (Fnull). Fisher’s inbreeding coefficient (Fis). Probability of identity (PI). Probability of exclusion (PE1, single parent; PE2, a second parent given a first parent assigned; PE3, a pair of parents). Number of repeated genotypes (Nrep and percentage (%) of the total number of individuals genotyped for each loci). Genotyping error rate per allele, E1 referring to allelic dropout rate and E2 to the false allele rate, and the 95% confidence interval (CI). Significant values are highlighted in bold (P<0.05) for heterozygote excess, Fnull, and Fis.</p><p>Summary statistics of the potentially selective «neutral» microsatellite markers (34) for albacore (<i>Thunnus alalunga</i>).</p

Read length distribution and number of reads throughout QDD bioinformatics pipeline steps.

<p>Read length distribution and number of reads throughout QDD bioinformatics pipeline steps.</p

Characteristics of two «non-neutral» microsatellite markers for albacore (<i>Thunnus alalunga</i>).

<p>Number of individuals (Nind). Number of alleles (A). Expected (He), unbiased Nei's (1978) expected (H.n.b) and observed (Ho) heterozygosity. Polymorphism information content (PIC). Null allele frequency (Fnull). Fisher’s inbreeding coefficient (Fis). Probability of identity (PI). Probability of exclusion (PE1, single parent; PE2, a second parent given a first parent assigned; PE3, a pair of parents). Number of repeated genotypes (Nrep and percentage (%) of the total number of individuals genotyped for each loci). Genotyping error rate per allele, E1 referring to allelic dropout rate and E2 to the false allele rate, and the 95% confidence interval (CI). Significant values are highlighted in bold (P<0.05) for heterozygote excess, Fnull, and Fis.</p><p>Characteristics of two «non-neutral» microsatellite markers for albacore (<i>Thunnus alalunga</i>).</p

Probability of detecting a particular level of differentiation (FST) among populations of albacore with 1 000 replicates.

<p>Probability of detecting a particular level of differentiation (FST) among populations of albacore with 1 000 replicates.</p

Summary of the selection steps used to develop microsatellite markers.

<p>Summary of the selection steps used to develop microsatellite markers.</p

Geographic location of albacore sampled.

<p>Circles are proportional to the number of individuals collected. (A) southwest Indian Ocean (n = 42), (B) northwest Indian Ocean (n = 31), (C) South Africa (n = 31), and (D) southeast Atlantic Ocean (n = 32).</p