A, Ethidium bromide stained 1% agarose gel electrophoresis of amplified MMTV-like sequences; B, Southern blot 13 hybridization of A using 5' Pend-labeled 23-mer probe

Lanes 2, 3 and 4 represent the amplified DNA from the daughter, mother and father respectively. C, Ethidium bromide stained 1.8 % agarose gel electrophoresis of amplified MMTV-like sequences; D, Southern blot [13] hybridization of C using 5' Pend-labeled 20 mer probe. Lanes 2, 3, and 4 represent amplified DNA from mother, daughter and father respectively. In Panels A-D, Lane 1 containing no template DNA and lanes 5, 6, and 7 containing normal breast tissue DNA from three separate individuals represent negative controls for sample contamination. M is the molecular weight marker ΦX174 RF DNA cut with the restriction enzyme .<p><b>Copyright information:</b></p><p>Taken from "Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter"</p><p>http://www.infectagentscancer.com/content/3/1/2</p><p>Infectious Agents and Cancer 2008;3():2-2.</p><p>Published online 28 Feb 2008</p><p>PMCID:PMC2277433.</p><p></p

The numbers 1, 2 and 3 indicate the three locations where the BR6 strain differs from the GR and C3H strains in this region of the MMTV gene 17,18,19

The and ↓ indicate the location at which the identical single base change described in the text occurs. The numbers 7656 and 7905 indicate the location of the MMTV 250 bp gene sequence within the MMTV genome [17]. Clones are designated as M (mother), D (daughter), and F (father) followed by a number (1–4) denoting the order in which they were cloned. - denotes the same nucleotide.<p><b>Copyright information:</b></p><p>Taken from "Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter"</p><p>http://www.infectagentscancer.com/content/3/1/2</p><p>Infectious Agents and Cancer 2008;3():2-2.</p><p>Published online 28 Feb 2008</p><p>PMCID:PMC2277433.</p><p></p

The 12 human MMTV-like sequences from the three family members as well as the human sequences previously isolated from human breast tumors, non-Hodgkin's lymphomas, and primary biliary cirrhosis tissue, clustered with their murine counterparts

Boxes denote sequences from mother (M-S1-4), daughter (D-S1-4), and father (F-S1-4). Previously published human isolates AF346815, AY325271, AF243039, AY652977, AY652968, AY652964, AY652975, AY652974, AY652967, AY652969, AY652973, from human breast tumors [6,16,27], AY652970, AY652976, AY652978, AY652965, AY652971, AY652966, AY652972 from human non-Hodgkin's lymphomas [6], and AF513913, AF513923, from human primary biliary cirrhosis patients [26,27]. The mouse sequences, JYG, FM, and SW21 from Asian mice that were used to root the tree, the endogenous MMTV proviral sequences Mtv-8, Mtv-1, and Mtv-6, and the exogenous MMTV sequences BR6, HEJ, and C3H are bolded. Numbers on branches indicate percent frequencies of assortment in an individual branch after the bootstrap procedure (45) and indicate the robustness of branch assignments. Branch lengths are indicative of the number of nucleotide changes to individual branch points (see scale bar).<p><b>Copyright information:</b></p><p>Taken from "Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter"</p><p>http://www.infectagentscancer.com/content/3/1/2</p><p>Infectious Agents and Cancer 2008;3():2-2.</p><p>Published online 28 Feb 2008</p><p>PMCID:PMC2277433.</p><p></p

Clones are named as M (mother), D (daughter), and F (father) followed by a dash and the letter s for and numbers (1–4) identifying the order in which they were cloned

- denotes the same nucleotide.<p><b>Copyright information:</b></p><p>Taken from "Mouse mammary tumor virus (MMTV)-like DNA sequences in the breast tumors of father, mother, and daughter"</p><p>http://www.infectagentscancer.com/content/3/1/2</p><p>Infectious Agents and Cancer 2008;3():2-2.</p><p>Published online 28 Feb 2008</p><p>PMCID:PMC2277433.</p><p></p