Additional file 1: of Partial Tmem106b reduction does not correct abnormalities due to progranulin haploinsufficiency

Figure S1. Measurement of Glycosylated HexA and GCase by Western Blot. The western blots in fig. 3 measured the glycosylated forms of HexA and GCase, which was confirmed by loss of these bands after digestion with the glycosidase PNGase F. The glycosylated forms of HexA and GCase are labeled with black arrows, the unglycosylated forms with gray arrows, and nonspecific bands with asterisks. (DOCX 195 kb

A novel methodology for strengthening human rights based monitoring in public health: Family planning indicators as an illustrative example

<div><p>Objective</p><p>The last few years have seen a rise in the number of global and national initiatives that seek to incorporate human rights into public health practice. Nonetheless, a lack of clarity persists regarding the most appropriate indicators to monitor rights concerns in these efforts. The objective of this work was to develop a systematic methodology for use in determining the extent to which indicators commonly used in public health capture human rights concerns, using contraceptive services and programmes as a case study.</p><p>Methods</p><p>The approach used to identify, evaluate, select and review indicators for their human rights sensitivity built on processes undertaken in previous work led by the World Health Organization (WHO). With advice from an expert advisory group, an analytic framework was developed to identify and evaluate quantitative, qualitative, and policy indicators in relation to contraception for their sensitivity to human rights. To test the framework’s validity, indicators were reviewed to determine their feasibility to provide human rights analysis with attention to specific rights principles and standards.</p><p>Findings</p><p>This exercise resulted in the identification of indicators that could be used to monitor human rights concerns as well as key gaps where additional indicators are required. While indicators generally used to monitor contraception programmes have some degree of sensitivity to human rights, breadth and depth are lacking.</p><p>Conclusion</p><p>The proposed methodology can be useful to practitioners, researchers, and policy makers working in any area of health who are interested in monitoring and evaluating attention to human rights in commonly used health indicators.</p></div

Steps to identify and Aasess human rights in public health indicators.

<p>Steps to identify and Aasess human rights in public health indicators.</p

Additional file 1: of Loss of Tmem106b is unable to ameliorate frontotemporal dementia-like phenotypes in an AAV mouse model of C9ORF72-repeat induced toxicity

Figures S1 through S10. Figure S1. Transcript expression of Tmem106b in Tmem106b deficiency mice at different ages. Figure S2. Tmem106b reduction does not alter the expression of its family members. Figure S3. Tmem106b immunoreactivity in mice with Tmem106b gene interruption using an additional antibody. Figure S4. The body weight of 2R and 66R injected mouse. Figure S5. Tmem106b reduction alone induces astrogliosis. Figure S6. Heterozygous loss of Tmem106b partially rescues 66R injection-induced neuronal loss. Figure S7. pTdp-43 immunoreactivity in 2R and 66R injected mouse brain. Figure S8. Endogenous C9orf72 protein levels in 2R- and 66R-injected mouse brain. Figure S9. Validation of (GGGGCC)66 repeat overexpression and C9ORF72 knockdown. Figure S10. The effect of (GGGGCC)66 overexpression or C9ORF72 knockdown on TMEM106B protein levels in U251 cells. (DOCX 26231 kb