Unraveling the periprandial changes in brain serotonergic activity and its correlation with food intake-related neuropeptides in rainbow trout Oncorhynchus mykiss

This study explored changes in brain serotonin content and activity together with hypothalamic neuropeptide mRNA abundance around feeding time in rainbow trout, as well as the effect of one-day fasting. Groups of trout fed at two (ZT2) and six (ZT6) hours after lights on were sampled from 90 minutes before to 240 minutes after feeding, while additional groups of non-fed trout were also included in the study. Changes in brain amine and metabolite contents were measured in hindbrain, diencephalon and telencephalon, while in the diencephalon the mRNA abundance of tryptophan hydroxylase ( tph1 , tph2 ), serotonin receptors (5htr1a , 5htr1b and 5htr2c ) and several neuropeptides ( npy , agrp1 , cartpt , pomca1 , crfb ) involved in the control of food intake were also assessed. The results showed changes in the hypothalamic neuropeptides that were consistent with the expected role for each in the regulation of food intake in rainbow trout. Serotonergic activity increased rapidly at the time of food intake in the diencephalon and hindbrain and remained high for much of the postprandial period. This increase in serotonin abundance was concomitant with elevated levels of pomca1 mRNA in the diencephalon, suggesting that serotonin might act on brain neuropeptides to promote a satiety profile. Furthermore, serotonin synthesis and neuronal activity appear to increase already before the time of feeding, suggesting additional functions for this amine before and during food intake. Exploration of serotonin receptors in the diencephalon revealed only small changes for gene expression of 5htr1b and 5htr2c receptors during the postprandial phase. Therefore, the results suggest that serotonin may play a relevant role in the regulation of feeding behavior in rainbow trout during periprandial time, but a better understanding of its interaction with brain centers involved in receiving and processing food-related signals is still needed.Agencia Estatal de Investigación | Ref. PID2022-136288OB-C31Xunta de Galicia | Ref. ED431B 2019/37Agencia Estatal de Investigación | Ref. BES-2017-079708Xunta de Galicia | Ref. ED481B-2022-08

Study of Local Power Dissipation in Ultrascaled Silicon Nanowire FETs

Abstract-The local electron power dissipation has been calculated in a field-effect nanowire transistor using a quantum transport formalism. Two different channel cross sections and optical and acoustic phonon mechanisms were considered. The phonon models used reproduce the phonon limited mobility in the cross sections studied. The power dissipation for different combinations of source, channel, and drain dimensions have been calculated. Due to the lack of complete electron energy relaxation inside the device, the Joule heat dissipation over-estimates the power dissipated in small nanotransistors. This over-estimation is larger for large cross sections due to the weaker phonon scattering. On the other hand, in narrow wires, the power dissipation inside the device can be large, therefore, mitigating against fabrication of very narrow nanowire transistors. We have also investigated the cooling of the device source region due to the mismatch of the Peltier coefficients between the source and the channel

Periprandial changes in brain serotonergic system and food intake related neuropeptides [Dataset]

In this work, we assessed periprandial serotonin and 5-hydroxyindolacetic acid abundance in several brain areas of rainbow trout, in parallel with the evaluation of gene expression of tryptophan hydroxilase 1 and 2, neuropeptides involved in the central control of food intake, and the quantification of plasma glucose and cortisol. The results showed severe changes in serotonergic systems around mealtime, the most prominent being the increase in their activity just after food intake, suggesting that serotonin has a relevant role in relation to the daily timing of food intake, probably triggering satiety signals. In addition, a temporal adjustment of neuropeptide expression and plasma cortisol was found in relation to food intake, supporting its role in the regulation of feeding behaviour.Agencia Estatal de Investigación, Award: PID2019-103969RBC31-C33. Xunta de Galicia, Award: ED431B 2019/37Peer reviewe

Evaluación de la eficacia del 2-fenoxietanol como anestésico en el lenguado (Solea senegalensis, Kaup, 1858)

En este trabajo se estudia la eficacia del anestésico 2-fenoxietanol (2-FE) y su potencial capacidad antiestrés en el lenguado (Solea senegalensis). Se determinaron las características de los diferentes estadios de inducción y recuperación de la anestesia, y el tiempo necesario para alcanzar los estadios II de inducción y de recuperación. Las dosis de 500 y de 600 mg/l fueron capaces de inducir el estadio II de anestesia en un tiempo inferior a los 5 minutos. Finalmente se comprueba que el uso del 2-FE (333 mg/l) es efectivo para atenuar un estrés de manipulación.Evaluation of the effectiveness of the 2-phenoxyethanol as anesthetic in the sole (Solea senegalensis, Kaup, 1858). In this work the effectiveness of the anesthetic 2-phenoxyethanol (2-PE) and its potential capacity anti-stress were studied in the sole (Solea senegalensis). The characteristics of the different stages of induction and recovery from the anesthesia, and the necessary time to reach the induction and recovery stages II from the anesthesia were studied. Doses of 500 and 600 mg/l were able to induce this stage II in less than 5 minutes. Finally it is verified that the use of 2-FE (333 mg/l) is effective to attenuate a manipulation stres