24 colour multifish karyotype showing the complexity of the genomic rearrangements with rearranged chromosomes in most pairs and several unidentified marker chromosomes (bottom left)

<p><b>Copyright information:</b></p><p>Taken from "Biological characterization of two xenografts derived from human CUPs (carcinomas of unknown primary)"</p><p>http://www.biomedcentral.com/1471-2407/7/225</p><p>BMC Cancer 2007;7():225-225.</p><p>Published online 18 Dec 2007</p><p>PMCID:PMC2241840.</p><p></p> Of particular interest are the translocation of chromosome 21 (in green) to the distal chromosome 4 (in grey) and the loss of chromosomes 9. In this cell, there were 2 der(3)t(3, 15) instead of one in most other cells which were analyzed

Microsatellite tracking assay linking DNA from patient peripheral blood mononuclear cells (PBMC), tumor surgical specimen (Surg

<p><b>Copyright information:</b></p><p>Taken from "Biological characterization of two xenografts derived from human CUPs (carcinomas of unknown primary)"</p><p>http://www.biomedcentral.com/1471-2407/7/225</p><p>BMC Cancer 2007;7():225-225.</p><p>Published online 18 Dec 2007</p><p>PMCID:PMC2241840.</p><p></p> Spec.) and xenograft to the Capi3 cell line

Nucleocytoplasmic distribution of chimeric MYC mRNAs, Northern blot analysis was performed on 10 μg of nuclear (lanes N) or cytoplasmic (lanes C) RNA extracted from 2G1MycP2Tu1 cells

<p><b>Copyright information:</b></p><p>Taken from "High frequency -splicing in a cell line producing spliced and polyadenylated RNA polymerase I transcripts from an rDNA- chimeric gene"</p><p>Nucleic Acids Research 2005;33(7):2332-2342.</p><p>Published online 22 Apr 2005</p><p>PMCID:PMC1084326.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> An MYC exon 3 or intron 1 probe was used, as indicated. Symbols are as in

Generalities.

(a)<p>CK, p = primary choriocarcinoma; CK, vm = vaginal metastasis from choriocarcinoma; CK, cl = choriocarcinoma cell line.</p>(b)<p>A = andromonospermic, AD = , B = , U =  unknown.</p

p-AML (case p24) with a complex karyotype.

<p>See the amplification of 15q23q24 and of 21q11.2q22.1 that are enlarged at the gene level. Multiple abnormalities (cf <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016623#pone-0016623-t005" target="_blank">table 5</a>) are asterisked.</p

Whole chromosome plots.

<p>Array-CGH from SK-N-DZ (A) and SIMA (B) cell lines. The X-axis represents the chromosomes while the Y-axis represent the normalize log2 ratio fluorescence intensity thresholds −1 (loss) and 1 (gain). The results show gains and losses of small chromosomal regions.</p

Minimal Critical Region in the two groups of patients.

<p>Column 1, the location of the MCR that follows the rules of <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016623#pone-0016623-t004" target="_blank">tables 4</a> and <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016623#pone-0016623-t005" target="_blank">5</a>; the figures in brackets and in bold are the ratios of the amplified regions. Column 2, patients; the figures in bold indicate the smaller CNA.</p

Gains and losses CNA in t-AML and revised karyotype after aCGH.

<p>Column 2 and 3: The CNA are either lost or gained as indicated by a “−” or a “+”; locations on chromosomes are described according to the ISCN 2009 with slight modifications: sequence numbers are included between <> and expressed in Mb, with a resolution of 10kb; linear ratios are written between brackets after an “×”; CNAs with a linear ratio >2 (low level of amplification) or losses <0.25 are labeled in <b>bold</b> and <b><i>italics</i></b>; “*” indicates CNA that are probably part of a rearrangement of the immunoglobulin genes. They have not been included in the synthetic karyotypes because they could be considered as an acquired CNV which is characteristic of monoclonal proliferation.</p><p>Column 4: In <b>bold</b> are new data or those modified by aCGH in the synthetic karyotypes; CNAs that were contiguous but whose ratios were not too different were fused to express overall chromosome abnormality for readability.</p

PANTHER classification by signalling pathway.

<p>The differentially expressed genes in both SK-N-DZ and SIMA CSC-like cells were classified by PANTHER and graphed. The percentage represents the number of genes altered against total number of genes involved in each pathway.</p

Gains and losses CNA in p-AML and revised karyotype.

<p>Column 2 and 3: The CNA were either lost or gained as indicated by a “−” or a “+”; the locations on the chromosomes are described according to the ISCN 2009 with slight modifications: sequence numbers are included between <> and expressed in Mb, with a resolution of 10kb; linear ratios are written between brackets after an “×”; CNAs with a linear ratio >2 (low level of amplification) or losses <0.25 are labeled in <b>bold</b> and <b><i>italics</i></b>; “*” indicates CNA that are probably part of a rearrangement of the immunoglobulin genes. They are not included in the synthetic karyotypes as they could be considered as an acquired CNV characteristic of monoclonal proliferation.</p><p>Column 4: In <b>bold</b> are new data or those modified by aCGH in the synthetic karyotypes; CNAs that were contiguous but whose ratios were not too different were fused to express overall chromosome abnormality for readability.</p