13 research outputs found
[70]Fullerenes Assists the Formation of Phospholipid Bicelles at Low Lipid Concentrations
The incorporation of neutral [70]fullerenes (C70) led to the bicelles formation at relatively low lipid concentration range from neutral lipid mixtures (DMPC/DHPC). Furthermore, the C70 addition resulted in the formation of large bicelles with a radius of ca. 100 nm in contrast to C70-free bicelles that were formed from anionic lipid mixtures (DMPC/DHPC/DMPG). The stabilization of these bicelles was attributed to C70 incorporation into the membranes.This file includes Supporting Information.This work was supported by JSPS KAKENHI a Grant-in-Aid for Scientific Research (B) (Grant No. 25288037), a Grant-in-Aid for Challenging Exploratory Research (Grant Nos. 24655128 and 25650053), and a Grant-in-Aid for Young Scientists (A) (Grant No. 24681028)
Improved photodynamic activities of liposomeâincorporated [60]fullerene derivatives bearing a polar group
[60]Fullerene (C60) derivatives were incorporated into liposomes using a fullerene exchange method involving the transfer of the fullerene from the cavity of two Îłâcyclodextrin molecules to a liposome. A lipidâmembraneâincorporated C60 derivative bearing a polar group showed much higher photodynamic activity than the analogous system incorporating pristine C60.This work was supported by JSPS KAKENHI GrantâinâAid for Scientific Research (B) (Grant No. JP16H04133) and GrantâinâAid for Challenging Exploratory Research (Grant No. JP16K13982)
Formation of βâ(1,3â1,6)âDâglucanâcomplexed [70]fullerene and its photodynamic activity towards macrophages
[70]Fullerene was dissolved in water by the complexation with βâ1,3âglucan using a mechanochemical highâspeed vibration milling apparatus. The photodynamic activity of βâ1,3 glucanâcomplexed C70 was highly dependent on the expression level of dectinâ1 on the cell surfaces of macrophages. The photodynamic activity increased as a result of a synergistic effect between βâ1,3âglucanâcomplexed 1ââacetoxychavicol acetate and the C70 complex.This work was supported by JSPS KAKENHI GrantâinâAid for Scientific Research (B) (Grant No. JP16H04133), GrantâinâAid for Scientific Research (C) (Grant No. 26350528) and GrantâinâAid for Challenging Exploratory Research (Grant No. JP16K13982)
Porphyrinâuptake in liposomes and living cells using an exchange method with cyclodextrin
The waterâsolubilisation of porphyrin derivatives is very important for biological applications. Although liposomal drug carriers for porphyrin derivatives have shown significant promise in the field of medicinal chemistry (e.g., as sensitisers for photodynamic therapy), it is currently not possible to prepare lipidâmembraneâincorporated tetraphenylporphyrin (TPP) with a high concentration of TPP using conventional methods. In this study, we have succeeded in preparing lipid-membraneâincorporated TPP and zinc(II) tetraphenylporphyrin (ZnTPP) from the corresponding TPP or ZnTPPâ˘cyclodextrin complex using the exchange method in lipidâmembranes composed of liposomes. Furthermore, the exchange method allowed for the incorporation of TPP or ZnTPP into the plasma membranes of HeLa cells. However, it was not possible to prepare lipidâmembraneâincorporated porphyrin derivatives with polar and hydrophilic groups in the meso positions using this exchange reaction.Electronic supplementary information (ESI) available: Experimental procedures, 1H NMR spectra, DLS measurements, cryo-TEM images, phase contrast and fluorescence images. See DOI: 10.1039/c5ra24985This work was supported by JSPS KAKENHI a GrantâinâAid for Scientific Research (B) (Grant No. 25288037) and a GrantâinâAid for Young Scientists (A) (Grant No. 24681028)
Gold Nanoparticle Inclusion into Protein Nanotube as a Layered Wall Component
We
describe the synthesis, structure, and catalytic activity of
human serum albumin (HSA) nanotubes (NTs) including gold nanoparticles
(AuNPs) as a layered wall component. The NTs were fabricated as an
alternating layer-by-layer assembly of AuNP and HSA admixture (a negatively
charged part) and poly-l-arginine (PLA, a positively charged
part) into a track-etched polycarbonate membrane (400 nm pore diameter)
with subsequent dissolution of the template. SEM images showed the
formation of uniform hollow cylinders of (PLA/AuNP-HSA)<sub>3</sub> with a 426 Âą 12 nm outer diameter and 65 Âą 7 nm wall thickness.
Transmission electron microscopy and energy dispersive X-ray measurements
revealed high loading of AuNPs in the tubular wall. HSAs bind strongly
onto the individual AuNP (<i>K</i> = 1.25 Ă 10<sup>9</sup> M<sup>â1</sup>), generating a coreâshell AuNP-HSA
corona, which is the requirement of the robust NT formation. Calcination
of the (PLA/AuNP-HSA)<sub>3</sub> NTs at 500 °C under air yielded
red solid NTs composed of thermally fused AuNPs. From the mass decrease
by heat treatment, we calculated the weight of the organic components
(PLA and HSA) and thereby constructed a six-layer model of the tube.
The (PLA/AuNP-HSA)<sub>3</sub> NTs serve as a heterogeneous catalyst
for reduction of 4-nitrophenol with sodium borohydrate. Furthermore,
implantation of the stiff (PLA/AuNP-HSA)<sub>3</sub> NTs vertically
onto glass plate produced uniformly cylindrical tube arrays
Regioselective Recognition of a [60]Fullerene-Bisadduct by Cyclodextrin
The
three different regioisomers of bis-<i>N</i>-methylfulleropyrrolidines
have been separated by controlling the relative amounts of Îł-cyclodextrin
and dimethyl sulfoxide (DMSO) contained in solutions of these compounds.
When a small amount of Îł-CDx was used in a mechanochemical high-speed
vibration milling apparatus, the <i>trans-1</i> and <i>trans-2</i>â˘Îł-CDx complexes were separated from
the <i>trans-3</i>â˘Îł-CDx complex. In contrast, <i>trans-3</i> was extracted in a relatively high ratio with an
excess of Îł-CDx. The addition of DMSO to aqueous solutions of
the fullerene derivativeâ˘Îł-CDx complexes allowed for
the three regioisomers to be obtained in high purity (>95%). The
basis
for the observed regioselective separation was a competition between
the relative stabilities and solubilities of the complexes in the
water and water-DMSO solvents. The stabilities of the complexes in
water were assessed by the number of hydrogen bonding interactions
between the two Îł-CDx units using molecular dynamics simulations.
To the best of our knowledge, this is the first reported example of
the isolation of the different regioisomers of fullerene derivatives
using hostâguest complexes
[70]Fullerenes Assist the Formation of Phospholipid Bicelles at Low Lipid Concentrations
The
incorporation of neutral [70]Âfullerenes (C<sub>70</sub>) led to bicelle
formation in a relatively low lipid concentration range from neutral
lipid mixtures (DMPC/DHPC). Furthermore, C<sub>70</sub> addition resulted
in the formation of large bicelles with a radius of ca. 100 nm, in
contrast to C<sub>70</sub>-free bicelles that were formed from anionic
lipid mixtures (DMPC/DHPC/DMPG). The stabilization of these bicelles
was attributed to C<sub>70</sub> incorporation into the membranes