26 research outputs found
Anthriscus nemorosa essential oil inhalation prevents memory impairment, anxiety and depression in scopolamine-treated rats
Anthriscus nemorosa (Bieb.) Sprengel is used for medicinal purposes in traditional medicine around the world, including Turkey. Ethnobotanical studies suggest that Anthriscus essential oil could improve memory in Alzheimer’s disease. The current study was hypothesized to investigate the beneficial effects of inhaled Anthriscus nemorosa essential oil on memory, anxiety and depression in scopolamine-treated rats. Anthriscus nemorosa essential oil was administered by inhalation in the doses of 1% and 3% for 21
continuous days and scopolamine (0.7 mg/kg) was injected intraperitoneally 30 min before the behavioral testing. Y-maze and radial arm-maze tests were used for assessing memory processes. Also, the anxiety and depressive responses were studied by elevated plus-maze and forced swimming tests. As expected, the scopolamine alone-treated rats exhibited the following: decrease the percentage of the spontaneous alternation in Y-maze test, increase the number of working and reference memory errors in radial arm-maze test, decrease of the exploratory activity, the percentage of the time spent and the number of entries in the open arm within elevated plus-maze test and decrease of swimming time and increase of immobility time within forced swimming test. However, dual scopolamine and Anthriscus nemorosa essential oil-treated rats showed significant improvement of memory formation and exhibited anxiolytic- and antidepressant-like effects in scopolamine-treated rats. These results suggest that Anthriscus nemorosa essential oil inhalation can prevent scopolamineinduced
memory impairment, anxiety and depression.Emel Aydin Akbaba was supported by LLP Erasmus (2013/2014), Alexandru Ioan Cuza University, Iasi, Romani
Antiamnesic and antioxidants effects of ferulago angulata essential oil against scopolamine-Induced memory impairment in laboratory rats
Ethical standard This study was approved by the Committee on
the Ethics of Animal Experiments of the Alexandru Ioan Cuza
University of Iasi (Permit Number: 2192) and also, efforts were made
to minimize animal suffering and to reduce the number of animals
used.Ferulago angulata (Apiaceae) is a shrub
indigenous to western Iran, Turkey and Iraq. In traditional
medicine, F. angulata is recommended for treating digestive
pains, hemorrhoids, snake bite, ulcers and as sedative.
In the present study, the effects of inhaled F. angulata
essential oil (1 and 3 %, daily, for 21 days) on spatial
memory performance were assessed in scopolamine-treated
rats. Scopolamine-induced memory impairments were
observed, as measured by the Y-maze and radial arm-maze
tasks. Decreased activities of superoxide dismutase, glutathione
peroxidase and catalase along with increase of
acetylcholinesterase activity and decrease of total content
of reduced glutathione were observed in the rat hippocampal
homogenates of scopolamine-treated animals as
compared with control. Production of protein carbonyl and
malondialdehyde significantly increased in the rat hippocampal
homogenates of scopolamine-treated animals as
compared with control, as a consequence of impaired
antioxidant enzymes activities. Additionally, in scopolamine-
treated rats exposure to F. angulata essential oil
significantly improved memory formation and decreased
oxidative stress, suggesting memory-enhancing and
antioxidant effects. Therefore, our results suggest that
multiple exposures to F. angulata essential oil ameliorate
scopolamine-induced spatial memory impairment by
attenuation of the oxidative stress in the rat hippocampusEmel (Aydin) Akbaba was supported by LLP Erasmus (2013/2014) during her stage within Alexandru Ioan Cuza University of Iasi, Romania
The effects of inhaled pimpinella peregrina essential oil on scopolamine-Induced memory impairment, anxiety, and depression in laboratory rats
Compliance with Ethical Standards Rats were treated in accordance
with the guidelines of the animal bioethics of the Act on Animal
Experimentation and Animal Health and Welfare from Romania and all
procedures were in compliance with Directive 2010/63/EU of the
European Parliament and of the Council of 22 September 2010 on the
protection of animals used for scientific purposes. This study was approved
by the Committee on the Ethics of Animal Experiments of the Alexandru
Ioan Cuza University of Iasi (Permit Number: 2192) and also, efforts were
made to minimize animal suffering and to reduce the number of animals
used.In the present study, we identified the effects of
inhaled Pimpinella peregrina essential oil (1 and 3 %, for 21
continuous days) on scopolamine-induced memory impairment,
anxiety, and depression in laboratory rats. Y-maze and
radial arm-maze tests were used for assessing memory processes.
Also, the anxiety and depressive responses were studied
by means of the elevated plus-maze and forced swimming
tests. The scopolamine alone-treated rats exhibited the following:
decrease of the spontaneous alternation percentage in
Y-maze test, increase of the number of working and reference
memory errors in radial arm-maze test, along with decrease of
the exploratory activity, the percentage of the time spent and
the number of entries in the open arm within elevated
plus-maze test and decrease of swimming time and increase
of immobility time within forced swimming test. Inhalation of
the P. peregrina essential oil significantly improved memory
formation and exhibited anxiolytic- and antidepressant-like
effects in scopolamine-treated rats. Our results suggest that
the P. peregrina essential oil inhalation ameliorates
scopolamine-induced memory impairment, anxiety, and depression.
Moreover, studies on the P. peregrina essential oil
may open a new therapeutic window for the prevention of neurological abnormalities closely related to Alzheimer’s
disease.Emel Aydin was supported by LLP Erasmus
(2013/2014), Alexandru Ioan Cuza University, Iasi, Romani
Anxiolytic and antidepressant-like effects of Ferulago angulata essential oil in the scopolamine rat model of Alzheimer’s disease
Ferulago angulata subsp. carduchorum (Apiaceae) is a shrub indigenous to western Iran, Turkey and Iraq. In traditional medicine, F. angulata is recommended for treating digestive pains, haemorrhoids, snake bites, ulcers and as a sedative. The present
study analysed the possible anxiolytic, antidepressant and antioxidant properties of F. angulata essential oil in a scopolamine-induced rat model of Alzheimer’s disease. The anxiolytic and antidepressant-like effects of F. angulata essential oil were studied using in vivo (elevated plus-maze and forced swimming tests) approaches. Also, the antioxidant activity in the amygdala was assessed using superoxide dismutase, glutathione peroxidase and catalase specific activities, the total content of the reduced glutathione, protein carbonyl and malondialdehyde levels. The scopolamine-treated rats exhibited the following: a decrease in the percentage of the time spent and the number of entries in the open armwithin the elevated plus-maze test and a decrease of swimming time and an increase of immobility time in the forced swimming test. Inhalation of F. angulata essential
oil significantly exhibited anxiolytic and antidepressant-like effects and also antioxidant potential. Furthermore, in silico studies carried out by employing molecular docking experiments pointed to the existence of strong interactions of monoterpenes from F. angulata essential oil with anxiolytic and antidepressant effects with GABAA receptor. Our results suggest that the F. angulata essential oil inhalation ameliorates scopolamine-induced anxiety and depression by attenuation of the oxidative stress in the rat amygdala. Copyright © 2015 John Wiley & Sons, Ltd.TÜBİTAK program 2221-Fellowships for Visiting Scientists and
Scientists on Sabbatical Leave (2014/2015) supported Prof. dr.
Lucian Hritcu during the collaboration stay in Firat University,
Elazig, Turkey
Behavioral effects of Hypericum uniglandulosum essential oil inhalation on rat model of Alzheimer’s disease
Hypericum (Hypericaceae) species have been attracting interest due to their sedative and
relaxing actions. In the current study, rats exposed to Hypericum uniglandulosum essential oil (HYP)
at the concentrations of 1.0%; and 3.0%, by inhalation during 15 minutes for 21 continuous days in
plexyglass boxes were evaluated on scopolamine model of Alzheimer’s disease. Memory-enhancing
effects of HYP were tested by well-characterized tasks namely Y-maze test and radial-arm maze test.
Furthermore, anxiolytic, and antidepressant effects of the inhaled essential oil were evaluated by
elevated plus maze and forced-swimming tests, respectively. As expected, scopolamine administration
decreased the spontaneous alternation percentage in Y-maze test, and working memory errors and
reference memory errors in radial-arm maze test. HYP 1% and HYP 3% administration in
scopolamine-induced rats caused statistically significant increases on the spontaneous alternation
percentage in Y-maze task. Number of arm entries do not show any significant differences between
scopolamine-alone treated and Sco+ HYP1% and Sco+HYP3% treated rats; thus, essential oil
treatment does not change the motor activity in rats. In order to evaluate spatial memory performances
further, radial-arm maze test was used. Scopolamine treatment showed an increase on the working
memory errors, a type of short-term memory, and reference memory errors, a type of long- term
memory, in this test. However, HYP inhalation significantly decreased these values. These results
show the spatial memory-enhancing activities of the inhaled HYP in scopolamine-treated rats. In
addition, anxiolytic and antidepressant-like effects of inhaled HYP were assessed. Diazepam, an
anxiolytic drug, significantly increased the time spent in the open arms, number of open arm entries
and number of crossings in the elevated plus maze task as compared to control rats. However,
scopolamine-alone treated rats showed decreases in these parameters. HYP 1% and HYP3% inhalation
increased these values in scopolamine –induced rats, therefore, acted as an anxiolytic drug. Forcedswimming
test was used to evaluate antidepressant-like behavior of rats. As expected, tramadol,
known antidepressant agent, increased the swimming time, and decreased the immobility time as
compared to control group. Scopolamine-alone treated rats exhibited significant decrease in the
swimming time, while increasing the immobility time. Both doses of HYP, but especially HYP1%
significantly decreased the immobility time and increased the swimming time as compared to
scopolamine-alone treated rats. In conclusion, Hypericum uniglandulosum essential oil inhalation was
showed to induce memory-enhancing, anxiolytic and antidepressant-like effects on scopolamineinduced
rats. These results suggest that Hypericum uniglandulosum essential oil could be used as an
alternative or complementary therapy against neurological diseases related to Alzheimer’s Disease
Memory-enhancing, anxiolytic and antidepressant effects of inhaled Achillea pseudoaleppica essential oil on scopolamine-induced amnesic rats
Achillea pseudoaleppica (Asteraceae) is an endemic plant species of Turkey. Several
species of the genus has been used in folk medicine in the treatment of wounds,
bleedings, headache, inflammation, pains, spasmodic diseases, flatulence and
dyspepsia. In the present study, memory-enhancing, anxiolytic and antidepressant-
like activities of A. pseudoaleppica essential oil on scopolamine-induced amnesic
rats are assessed. In order to evaluate memory- enhancing activities of A.
pseudoaleppica essential oil (1% and 3%, for 21 continuous days) Y-maze and
radial arm maze tasks were used. As expected, scopolamine administration
decreased spontaneous alternation percentage in Y-maze test and increased the
number of working memory errors and the number of reference memory errors in
radial arm-maze test. A. pseudoaleppica essential oil used in this study displayed
an improved effect on acquisition of the short-term memory of scopolamine-treated
rats within the Y-maze task as evidenced by increased spontaneous alternation
percentage. A. pseudoaleppica essential oil administered rats also showed
significant improvement of reference memory formation, but not working memory
formation in radial arm maze test. Furthermore, A. pseudoaleppica essential oil
induced anxiolytic effect on elevated plus maze test as evidenced by increased
percentage of open arm time, open arm entries and the number of crossings as
compared to scopolamine-alone treated rats. A. pseudoaleppica essential oil also
exhibited antidepressant-like effects as evidenced by decreased immobility time on
forced-swimming test. These results suggest the neuroprotective potential of
Achillea pseudoaleppica essential oil on scopolamine-induced amnesia in rats and
could act as a promising therapeutic agent for the neurological abnormalities
related to Alzheimer's disease
Behavioral effects of Hypericum uniglandulosum essential oil inhalation on rat model of Alzheimer’s disease
Hypericum (Hypericaceae) species have been attracting interest due to their sedative and
relaxing actions. In the current study, rats exposed to Hypericum uniglandulosum essential oil (HYP)
at the concentrations of 1.0%; and 3.0%, by inhalation during 15 minutes for 21 continuous days in
plexyglass boxes were evaluated on scopolamine model of Alzheimer’s disease. Memory-enhancing
effects of HYP were tested by well-characterized tasks namely Y-maze test and radial-arm maze test.
Furthermore, anxiolytic, and antidepressant effects of the inhaled essential oil were evaluated by
elevated plus maze and forced-swimming tests, respectively. As expected, scopolamine administration
decreased the spontaneous alternation percentage in Y-maze test, and working memory errors and
reference memory errors in radial-arm maze test. HYP 1% and HYP 3% administration in
scopolamine-induced rats caused statistically significant increases on the spontaneous alternation
percentage in Y-maze task. Number of arm entries do not show any significant differences between
scopolamine-alone treated and Sco+ HYP1% and Sco+HYP3% treated rats; thus, essential oil
treatment does not change the motor activity in rats. In order to evaluate spatial memory performances
further, radial-arm maze test was used. Scopolamine treatment showed an increase on the working
memory errors, a type of short-term memory, and reference memory errors, a type of long- term
memory, in this test. However, HYP inhalation significantly decreased these values. These results
show the spatial memory-enhancing activities of the inhaled HYP in scopolamine-treated rats. In
addition, anxiolytic and antidepressant-like effects of inhaled HYP were assessed. Diazepam, an
anxiolytic drug, significantly increased the time spent in the open arms, number of open arm entries
and number of crossings in the elevated plus maze task as compared to control rats. However,
scopolamine-alone treated rats showed decreases in these parameters. HYP 1% and HYP3% inhalation
increased these values in scopolamine –induced rats, therefore, acted as an anxiolytic drug. Forcedswimming
test was used to evaluate antidepressant-like behavior of rats. As expected, tramadol,
known antidepressant agent, increased the swimming time, and decreased the immobility time as
compared to control group. Scopolamine-alone treated rats exhibited significant decrease in the
swimming time, while increasing the immobility time. Both doses of HYP, but especially HYP1%
significantly decreased the immobility time and increased the swimming time as compared to
scopolamine-alone treated rats. In conclusion, Hypericum uniglandulosum essential oil inhalation was
showed to induce memory-enhancing, anxiolytic and antidepressant-like effects on scopolamineinduced
rats. These results suggest that Hypericum uniglandulosum essential oil could be used as an
alternative or complementary therapy against neurological diseases related to Alzheimer’s Disease
Composition of essential oil two medicinal plants (Eucalyptus camaldulensis dehnh. and vibirnum opulus l.)
Eucalyptus is one of the world’s important and most widely planted species belongs to the
family Myrtaceae. Several species of Eucalyptus are used in folk medicine as an antiseptic and against
infections of the upper respiratory tract, such as cold, influenza and sinus congestion. The essential oil
of Eucalyptus species showed a wide spectrum of antimicrobial, antifungal, anticandidal, antibacterial,
expectorant and cough stimulant activity. In this study, the chemical composition of the essential oil
from the leaves and fruits of Eucalyptus camaldulensis Dehnh. and aerial parts of Vibirnum opulus L.
grown in Mersin (Turkey) were analyzed by using GC and GC/MS techniques. The yields of essential
oils were 1.2% in leaf and 1.0% (v/w) in fruit of E. camaldulensis and identified representing 93.8%
and 99.0% of the total oils, respectively. The major constituents of leaves were p-cymene (42.1%),
eucalyptol (1,8-cineole, - -terpinol (10.7%), and in fruit were
eucalyptol (1,8 cineole) (34.5%), p- -terpinol (15.1%). Our results showed that
both oils has rich in terms of monoterpene hydrocarbons and oxygenated monoterpenes. Viburnum
opulus is from Adoxaceae family and it is reported that has anticancer, antiaging and antioxidant
activity. This species used for renal stone reduction. The yields of essential oil of V. opulus was 0.5%
- -
pinene (27.2%) and butanoic acid (15.8%) were the main compounds identified in the oil of Vibirnum
opulus