Development and validation of a chromatographic method for quantification of rasagiline in human plasma

Purpose: To develop a sensitive, reliable and cost-effective bioanalytical method for the pharmacokinetic analysis of rasagiline in human plasma.Method: Rasagiline was extracted by liquid-liquid extraction method and analyzed by reversed-phase high performance liquid chromatography (HPLC) using a mixture of ammonium acetate (pH 5.8) and acetonitrile (55:45, v/v) as mobile phase at a flow rate of 1 mL/min. The separation was performed on a Lichrosphere reverse-phase (RP) C18 column (250 x 4.6 mm, 5 μm particle size) at ambient temperature and rasagiline was detected at a wavelength of 265 nm by ultra-violet UV detection. The method was validated according to European Medicine Agency (EMA) guidelines. Results: The developed method was linear over a concentration range of 0.5 - 20 μg/ml with r2 ≥ 0.999 in human plasma. Run time was 10 min with rasagiline peak appearing at 7 min with no interference. Relative recovery and relative standard deviation (RSD) for accuracy and precision were within the acceptable limits prescribed in EMA guidelines. Rasagiline remained stable in human plasma for 24 h at room temperature, after three freeze and thaw cycles and also for 3 months at -20 °C.Conclusion: A simple and reliable method has been successfully developed and validated for the determination of rasagiline concentration in human plasma.Keywords: Rasagiline, Pharmacokinetics, Validation, Parkinson's diseas

An updated review on pharmacological activities and phytochemical constituents of evening primrose (genus Oenothera )

International audienceGenus Oenothera includes medicinal plants that are distributed throughout the world and are known since ancient times. Popular indications of different species of this genus include treatment of inflammations, diabetes, microbial infections, ulcers, tumors, kidney and liver problems. The plants of this genus are a botanical source for various pharmaceutically active components like sterols, alkaloids, phenolic acids, flavonoids, triterpe-noids, saponins, biflavonols and tocopherols. This review article is a compilation of chemical composition and biological activities of the various species of the genus Oenothera

Anti-Ulcerogenic Evaluation of Torilis Leptophylla Plant Extract on Indomethacin Induced Mice Gastric Ulcer

Introduction: Despite conventional anti-ulcer therapies for peptic ulcer diseases, medicinal plants might provide effective new anti-ulcer compounds or, alternatively, as adjuncts to existing therapies. Aims & Objectives: To evaluate the effects of Torilis leptophylla on indomethacin-induced gastric ulcer in mice. Place and duration of study: It was an experimental study carried out in the Department of Pharmacology, University of Health Sciences, Lahore, from March to December 2016. Material & Methods: Thirty six (36) adult healthy male BALB/C mice were divided equally in 6 groups and assigned as group I (control), group II (positive control), group III–V (TLM low, medium and high dose) and group VI (omeprazole). Gastric ulcers were induced by oral ingestion of indomethacin in groups II -VI. Acute oral toxicity of the plant was also tested. Antiulcer effect was assessed by measuring body weight, amount and pH of gastric juice, ulcer count, severity of gastric lesion, ulcer index, percentage (%) inhibition of ulcer and histopathology of gastric tissue. Results were analyzed by SPSS 20.0, P-value<0.05 was considered significant. Results: Gastric ulcer reduced the body weight in indomethacin induced animals (28 ± 2.53, 29.66 ± 3.88, 29.66 ± 2.33, 31 ± 3.52,32 ± 3.099 g in group II, III, IV, V and VI respectively) at day 3. Omeprazole and TLM treated groups reduced the gastric volume and pH as compared to positive control. Ulcer index (18.83, 5.14, 3.42, 1.71, 1.76 of the group II, III, IV, V and VI respectively) depicted significant reduction by treatment groups. Ulcer's percentage inhibition (72.7, 81.8, 90.88, 90.65 of low, median and high dose of TLM and standard drug respectively) was increased. Histopathological observations were remarkably reversed by TLM treated groups. Conclusion: Torilis leptophylla could significantly protect gastric mucosa from damage by indomethacin

SARS-CoV-2 vaccination modelling for safe surgery to save lives: data from an international prospective cohort study

Background: Preoperative SARS-CoV-2 vaccination could support safer elective surgery. Vaccine numbers are limited so this study aimed to inform their prioritization by modelling. Methods: The primary outcome was the number needed to vaccinate (NNV) to prevent one COVID-19-related death in 1 year. NNVs were based on postoperative SARS-CoV-2 rates and mortality in an international cohort study (surgical patients), and community SARS-CoV-2 incidence and case fatality data (general population). NNV estimates were stratified by age (18-49, 50-69, 70 or more years) and type of surgery. Best- and worst-case scenarios were used to describe uncertainty. Results: NNVs were more favourable in surgical patients than the general population. The most favourable NNVs were in patients aged 70 years or more needing cancer surgery (351; best case 196, worst case 816) or non-cancer surgery (733; best case 407, worst case 1664). Both exceeded the NNV in the general population (1840; best case 1196, worst case 3066). NNVs for surgical patients remained favourable at a range of SARS-CoV-2 incidence rates in sensitivity analysis modelling. Globally, prioritizing preoperative vaccination of patients needing elective surgery ahead of the general population could prevent an additional 58 687 (best case 115 007, worst case 20 177) COVID-19-related deaths in 1 year. Conclusion: As global roll out of SARS-CoV-2 vaccination proceeds, patients needing elective surgery should be prioritized ahead of the general population