Preparation of inactivated vaccine to induce resistance against white spot syndrome virus by nuclear & non nuclear techniques

The inactivated of white spot syndrome virus (WSSV) by Gama and Electron radiation and chemical material such as Formalin with antigen property have been done during last year. At the first time the stock of virus prepared and purred it. Six hundred of shrimp Fenneropenaus indicus with average weight 7 to 12 gram collected from shrimp research station in Heleh area and transported to Shrimp Research Institute in Boushehr province. Vibrio parahemolyticus obtained from Veterinary University of Tehran and then lyophilized it. The bacterial V. parahemolyticus inactivated with Gamma, electron and formalin and then injected to shrimp for activated the immune system. The source of viruses was identified through PCR, TEM and histopathology methods from the shrimp infected in 2009 occurrence of boushehr province. The virus injected to crayfish and collected the hemlymph for prepared the stock of WSSV virus. The virus was lyophilized and then exposed to gamma and electron radiation and formalin with optimal dose for inactivated the virus. The shrimp divided to 25 groups and each group consist of six shrimp and the vaccine exposed to shrimp with injected and bathroom methods and the documented the result after 15 days. In vivo virus titration was performed in Penaeus indicus. Inactivation of WSSV was carried out by a gamma cell instrument Nordian, model 220 with dose rate: 4.8 Gy/sec and activity: 20469 Ci. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of gamma radiation beam to inactivate WSSV was obtained 14-15 kGy. The LD50 of live virus stock was calculated 10 5.4 / ml and the optimum dose of electron beam to inactivate WSSV was obtained 12-13 kGy and and the virus inactivated by formalin 0.5% V/V during 10 minutes.The LD50 of live virus stock was calculated by Karber method 10 3.29 /ml and 10 5.35 /ml, respectively. The V. parahemolyticus was inactivated with 8 KG. The result showed all vaccine during 2 hours have a good effect to shrimp viruses with the 102.4 LD50 / 50 l titre. The result showed if the Gamma and electron vaccine increase to feed of shrimp the effect of vaccine is better than when used without feed. The end of experiment our study showed that the Gamma vaccine has the better effect to control WSSV during 2 hours with comparing the others

MR Vaccination in Pregnancy and its Subsequent Complications

Background and purpose: MR vaccination is prohibited among pregnant women, therefore pregnancy is recommended three months after vaccination. On the other hand, pregnant women acquiring these diseases face unwanted complications. We tried to determine the frequency of congenital disorders in the newborns of the vaccinated pregnant women under 25 years in Mazandaran province.Materials and Methods: This historical cohort study was done by consent method on 1031 cases throughout Mazandaran province. 406 cases out of the population were vaccinated without prior information about their pregnancy. The data were collected by questionnaires through referring to their healthcare files and telephone contacts just to make sure about their vaccination conditions. The data obtained from these cases along with the data from 493 pregnant women who were not vaccinated during the years 2002-2003 were statistically analyzed.Results: Pregnancy complications were observed in 58 (6.5%) subjects; 24 (41.38%) in the control group and 34 (58.62%) in the case group. The rate of complications in the subjects under the study was as follows: premature delivery in 27 (46.57%) subjects; 8 (29.62%) in the control group and 19 (70.38%) in the case group; weight under 2500 gram at birth in 19 (32.75%) subjects; 12 (63.16%) in the control group and 7 (36.84%) in the case group; still birth in 6 (10.34%) subjects 1 (16.67%) in the control group and 5 (83.33%) in the case group; abortion in 6 subjects (10.34%); 2 (23.33%) in the control group and 4 in (66.67%) in the case group. Only one person in the control group had intra uterine growth retardation. No significant pregnancy complication difference was observed between the case and the control groups in this study (P>0.05)Conclusion: Results of this study and the related researches indicate that though MR vaccination during pregnancy is safe, the randomly performed vaccination of pregnant women, should be advised and the followed up for the probable unwanted complications