Plasma antioxidant responses and oxidative stress following a 20 meter shuttle run test in female volleyball players

The effect of physical exercise on oxidant stress and antioxidants has been investigated extensively in the last twenty years. Cells continuously produce free radicals under normal conditions during mitochondrial electron transport chain (ETC). Experimental studies have shown elevated metabolic rate by strenuous physical exercise induces oxidative stress and production of excessive amounts of free radicals. Lipid peroxidation occurs when free radicals react with cellular components involving polyunsaturated fatty acid residues of phospholipids which are very sensitive to oxidation. This study aimed to determine plasma antioxidant responses and oxidative stress following a 20 meter shuttle run test in female volleyball players. Ten female volleyball players from the same team, and ten sedentary female ages between 18-24 years old volunteered to participate in this study. They were in good health and 48 hours before the test did not receive any drug or alcohol. None of them had any endocrine, orthopedic problems. Before the study, Informed, written consent was obtained from all the participants after full explanation of the procedures involved. All procedures were approved by the Selçuk University Meram Medical School of Ethical Committee. 20 meter shuttle run test was designed to estimate the maximal aerobic power of athletes performing in sports with frequent stops and starts (eg. Basketball, volleyball, fencing and so on). Findings of our study demonstrate that in both female groups 20 meter shuttle run test leads to production of more reactive oxygen species than the antioxidant systems can scavenge. Decrease in the activities of these antioxidant enzymes may be due to their inactivation caused by the higher production of the free radicals. it seems that the vulnerability of the body to oxidative stress is significantly enhanced after strenuous exercise test.The effect of physical exercise on oxidant stress and antioxidants has been investigated extensively in the last twenty years. Cells continuously produce free radicals under normal conditions during mitochondrial electron transport chain (ETC). Experimental studies have shown elevated metabolic rate by strenuous physical exercise induces oxidative stress and production of excessive amounts of free radicals. Lipid peroxidation occurs when free radicals react with cellular components involving polyunsaturated fatty acid residues of phospholipids which are very sensitive to oxidation. This study aimed to determine plasma antioxidant responses and oxidative stress following a 20 meter shuttle run test in female volleyball players. Ten female volleyball players from the same team, and ten sedentary female ages between 18-24 years old volunteered to participate in this study. They were in good health and 48 hours before the test did not receive any drug or alcohol. None of them had any endocrine, orthopedic problems. Before the study, Informed, written consent was obtained from all the participants after full explanation of the procedures involved. All procedures were approved by the Selçuk University Meram Medical School of Ethical Committee. 20 meter shuttle run test was designed to estimate the maximal aerobic power of athletes performing in sports with frequent stops and starts (eg. Basketball, volleyball, fencing and so on). Findings of our study demonstrate that in both female groups 20 meter shuttle run test leads to production of more reactive oxygen species than the antioxidant systems can scavenge. Decrease in the activities of these antioxidant enzymes may be due to their inactivation caused by the higher production of the free radicals. it seems that the vulnerability of the body to oxidative stress is significantly enhanced after strenuous exercise test

Elit taekwondocularda c vitamini uygulamasının IFN-γ, TNF-α, IL-2 ve IL-6 düzeylerine etkisi

Amaç: Bu çalışmanın amacı, taekwondo sporu yapan bireylerde C vitamini uygulamasının sitokin salınımı üzerindeki etkilerinin araştırılmasıdır. Gereç ve Yöntem: Bu araştırma Selçuk Üniversitesi Beden Eğitimi ve Spor Yüksekokulunda okuyan yaş ortalaması; 20.67±0.24 yıl, vücut ağırlığı ortalaması; 65.45±1.69 kg olan 10 adet erkek elit taekwondocu üzerinde gerçekleştirildi. Deneklere 4 hafta süreyle günde oral olarak 300 mg C vitamini tablet şeklinde verildi ve 4 haftalık vitamin ilavesi öncesi ve sonrası olmak üzere iki kez yorgunluk oluşuncaya kadar tükenme egzersizi yaptırıldı. Dört hafta süren uygulama öncesinde ve sonrasında deneklerden istirahat ve yorgunluk olmak üzere 4 kez kan örnekleri alındı. Alınan kan örneklerinde serumda interferon gama IFN-γ , tümör nekrosiz faktör-alfa TNF-α , interlökin-2 IL-2 ve interlökin-6 IL-6 düzeyleri ELISA test kitleri kullanılarak tayin edildi. Bulgular: IFN-γ, TNF-α ve IL-2 düzeyleri hem uygulama öncesi hem de sonrası anlamlı bir farklılık göstermedi p>0.05 . Uygulamadan önceki egzersiz periyodunda serum IL-6 düzeylerinde dinlenme periyoduna göre anlamlı artış vardı