The prevalence of helicobacter pylori in referral population of Turkey

Helicobacter pylori infection is commonly associated with gastroduodenal diseases in humans, such as chronic gastritis and peptic ulcers, gastric mucosa-associated lymphoid tissue lymphoma, and even gastric cancer, which leads to high cost to society for treatment and even to death many people, when people do not know early of the infection prevalence. In this work we proposed a forecasting model to predict the infection prevalence. Based on our results society can make simple early prevention acts against the infection. The early prevention acts decrease the cost of treatment and save many people's lives in the world

Sensitive detection and quantification of SARS-CoV-2 in saliva

Abstract Saliva has significant advantages as a test medium for detection of SARS-CoV-2 infection in patients, such as ease of collection, minimal requirement of supplies and trained personnel, and safety. Comprehensive validation in a large cohort of prospectively collected specimens with unknown SARS-CoV-2 status should be performed to evaluate the potential and limitations of saliva-based testing. We developed a saliva-based testing pipeline for detection of SARS-CoV-2 nucleic acids using real-time reverse transcription PCR (RT-PCR) and droplet digital PCR (ddPCR) readouts, and measured samples from 137 outpatients tested at a curbside testing facility and 29 inpatients hospitalized for COVID-19. These measurements were compared to the nasal swab results for each patient performed by a certified microbiology laboratory. We found that our saliva testing positively detects 100% (RT-PCR) and 93.75% (ddPCR) of curbside patients that were identified as SARS-CoV-2 positive by the Emergency Use Authorization (EUA) certified nasal swab testing assay. Quantification of viral loads by ddPCR revealed an extremely wide range, with 1 million-fold difference between individual patients. Our results demonstrate for both community screening and hospital settings that saliva testing reliability is on par with that of the nasal swabs in detecting infected cases, and has potential for higher sensitivity when combined with ddPCR in detecting low-abundance viral loads that evade traditional testing methods