СИНТЕЗ ФОСФАТИДИЛЬНОГО ПРОИЗВОДНОГО КИНЕТИНРИБОЗИДА С ИСПОЛЬЗОВАНИЕМ ФОСФОЛИПАЗЫ D STREPTOMYCES NETROPSIS

Herein, the environmentally friendly synthesis of the earlier unknown phospholipid analog of kinetin riboside was carried out using phospholipase D of Streptomyces netropsis. Kinetin riboside and soybean lecithin served as substrates. A maximum degree of nucleoside conversion to 5′-phosphatidyl derivative of kinetin riboside at 37°С to 6 h exceeds 95%. The structure of target product was confirmed by UV and 1Н NMR spectroscopy.С помощью фосфолипазы D Streptomyces netropsis проведен экологически безопасный синтез ранее неизвестного фосфолипидного аналога кинетинрибозида. В качестве субстратов были использованы кинетин и соевый лецитин. Максимальная степень трансформации нуклеозида в 5′-фосфатидильного производного кинетинрибозид при 37 °С за 6 ч реакции превышала 95 %. Структура целевого продукта доказана УФ- и 1Н ЯМР-спектроскопией

31Р-ЯМР-спектроскопический контроль получения фосфолипидов при катализе фосфолипазой D

To monitor the process of enzymatic reaction,31P-NMR spectroscopic technique has been proposed as an alternative to chromatographic method. Using this approach, the time-course of soybean phosphatidylcholine (lecithin) biotransformation into phosphatidylserine or phosphatidylglycerol catalyzed by Streptomyces netropsis BIM В-235 phospholipase D has been established.Предложен альтернативный хроматографическим методам слежения за ходом ферментативной реакции подход с применением31P-ЯМР-спектрометрии. С помощью этого метода охарактеризована динамика процесса биокаталитической трансформации фосфатидилхолина из соевых бобов в фосфатидилсерин или фосфатидилглицерин под действием фосфолипазы D Streptomyces netropsis БИМ В-235

Influence of coal sorbents on water molecules dynamics

The results of investigations of the effect of non- and porous sorbents on water dynamics have been discused. Characteristics of dynamics by the spectra was obtained of quasi-elastic slow neutron scattering have been calculated. The total coefficient of self-diffusion of water molecules the contributions to it from collective (Lagrange) motion and single-particle (Frankel) motion; molecule lifetime at oscillation state and length of molecule jump from one to another equilibrium center have been quantitative estimated. Nonporous sorbents don't effect on water dynamics but porous sorbents decrease total coefficient of self-diffusion at the constant value of the contribution from collective motion has been established for the condition of experiment

Quasielastic scattering of slow-neutron in water-alcohol solutions

Research of molecules dynamics of solutions “water - propyl alcohol” of different concentration at the temperature 281 K is conducted by the method of slow-neutron quasi-elastic scattering. There were experimentally exposed the feature of effective self-diffusion coefficient of molecules of the indicated solutions. Based on the time- scale hierarchy the division of selfdiffusion coefficient to one-particle and collective contributions was conducted, and the time of the molecules settled life in position of equilibrium was calculated. There were also exposed the feature of self-diffusion concentration dependence of coefficient of self-diffusion and his selfpart contribution, namely: presence of two minimums is in the areas of concentrations (0,04 ÷ 0,05) of mass fraction and (0,18 ÷ 0,22) m.c. of the alcohol and continuous character of diffusion at concentrations higher then 0,4 m.c. of the alcohol. It is shown that the indicated concentration areas correspond the certain local structures of investigational solution

Multichannel analyser for the neutron time-of-flight spectrometer

New multichannel time-of-flight spectrometer for the measurement of the energy and angular distributions of neutrons from the WWWR-M reactor is considered. This spectrometer has been developed for the replacement of the previous one to increase the number of channels and measurement precision, reduce the time of channel tuning and provide the automatic monitoring during the experiment