Oncopeltus fasciatus zen is essential for serosal tissue function in katatrepsis

AbstractUnlike most Hox cluster genes, with their canonical role in anterior–posterior patterning of the embryo, the Hox3 orthologue of insects has diverged. Here, we investigate the zen orthologue in Oncopeltus fasciatus (Hemiptera:Heteroptera). As in other insects, the Of-zen gene is expressed extraembryonically, and RNA interference (RNAi) experiments demonstrate that it is functionally required in this domain for the proper occurrence of katatrepsis, the phase of embryonic movements by which the embryo emerges from the yolk and adjusts its orientation within the egg. After RNAi knockdown of Of-zen, katatrepsis does not occur, causing embryos to complete development inside out. However, not all aspects of expression and function are conserved compared to grasshopper, beetle, and fly orthologues. Of-zen is not expressed in the extraembryonic tissue until relatively late, suggesting it is not involved in tissue specification. Within the extraembryonic domain, Of-zen is expressed in the outer serosal membrane, but unlike orthologues, it is not detectable in the inner extraembryonic membrane, the amnion. Thus, the role of zen in the interaction of serosa, amnion, and embryo may differ between species. Of-zen is also expressed in the blastoderm, although this early expression shows no apparent correlation with defects seen by RNAi knockdown

Preparation and Characterization of Organic Nanoparticles of Oxadiazole Derivative in Aqueous Media

A novel  oxadiazole derivative, 2,5-disubstituted-1,3,4-oxadiazole (Z)-4-((1-(5-(4-hydroxyphenyl)-1,3,4-oxadiazol-2-yl)prop-1-en-2-yl)amino)-1,5-dimethyl-2-phenyl-1H-pyrazol-3(2H)-one (HOPA) was synthesized from reaction of (Z)-3-[(1,5-dimethyl-3-oxo-2-phenyl-2,3-dihydro-1H-pyrazol-4-yl)amino]but-2-enehydrazide, with 4-hydroxybenzaldehyde in the presence of iodine as catalyst .The structure of HOPA was characterized by using IR,1H-NMR and mass spectroscopy. Nanoparticles of the prepared compound HOPA have been fabricated in aqueous media using re-precipitation method. Characterizations of the organic nanoparticles (ONPs) have been performed by using UV-Vis spectrophotometry, and scanning electron microscope (SEM). The HOPA NPs were approximately monodisperse even without using capping agent or surfactant. The size of the nanoparticles were around 150-200 nm as indicated by SEM. The maximum of absorption spectrum of the dispersed nanoparticles is red shifted by 5 nm from the molecular absorption spectra of the HOPA in the solution, which indicate head to head alignment (J aggregate) of the molecules of HOPA during the aggregation. The well dispersed organic nanoparticles in aqueous media holds potential application in future to be used as a tag for biomedical imagining. Keywords: Nanoscience, Organic Nanoparticle, Reprecipitation, Oxdiazoles

A numerical model for predicting the aerodynamic characteristics of propelling nozzles

It is essential to predict the exhaust-system performance of the aero-engine during the design stages as it plays a critical role in the engine components matching. In addition to this, it has an impact on the overall engine performance. Consequently, it is important to model the complex flow features around the exhaust system accurately in order to capture the flow characteristics. Computational Fluid Dynamics (CFD) alongside with low-order models can play a central role in the design and performance assessment of the propulsion system. This paper aims to explore the suitability of a numerical model, boundary conditions, and the employed mesh topology in computing a propelling nozzle performance. The current work is a first step towards building a module to assess a wide range of nozzle configurations at the preliminary design stages. A single-stream and plug-nozzle propelling nozzle were simulated for this purpose. For the single-stream nozzle, the simulations were run at various flight conditions and different geometrical features. For both nozzle configurations, a comparison between the effectiveness of six turbulence models to capture the nozzle flow features is presented. The validated module is then used to assess the impact of the bypass flow and the plug half-angle on the performance of the core nozzle for a dual-stream nozzle configuration. The calculated nozzle efficiencies are lower than the experimental data for both nozzle types, with a maximum difference of single-stream nozzle efficiency ≈ - 3.29% at NPR = 1.83 and by -0.84% at NPR = 3.88 and for the plug nozzle with -1.05% at NPR 2.64 and across a range from -0.46% to -0.68% between NPR = 3.14 to 5.3. The application of RANS k-ω SST turbulence model showed the best results as compared with the standard k-ε, RNG k-ε, realizable k-ε, and Spalart-Allmaras models in simulating the propelling nozzles aerodynamics. Generally, the results show the strength and the weakness of the numerical module in simulating the nozzle flow features and predicting its performance. Moreover, the Fan Nozzle Pressure Ratio (FNPR) and the plug half-angle (ω) has a noticeable impact on the overall and core nozzle performance. Moreover, the combined impact of both parameters has a noticeable impact on the propelling nozzle performance

Some Behavioural Responses Of Limicolaria aurora Exposed To Gramoxone

The effects of 0.5, 1.0, 1.5 and 2.0 % of combined oral and dermal single-application of 0.1 M concentration of gramoxone (contact herbicide, paraquat chloride) on Limicolaria aurora were determined and compared with control using Amaranthus sp. as bait. Responses were measured through normal feeding and crawling, head retraction into the shell, swelling, excessive mucus secretion, lack of response to mechanical stimuli (mortality). Results showed no effects on controls. Snails exposed to gramoxone fed less as contamination increased. Gramoxone is molluscicidal, it can also result in behaviour–modifying observations in snails which are non-target organisms in the ecosystem. Key words: African giant land snail, gramoxone, ecotoxicology. Nigerian Journal of Physiological Sciences Vol.19(1&2) 2004: 82-8

The use of enhanced nozzle maps for gas-turbine performance modelling

The use of a simulation tool to predict the aero-engine performance before committing to a final engine design has become one of the most cost-saving approaches in this field. However, most of these tools are based on low fidelity thermodynamic models, which are incapable of fully capturing the impact of three-dimensional flow characteristics. An aero-engine exhaust-system is one of the essential components that affect the engine performance. Currently, engine performance models tend to utilize simplified nozzle performance maps. These maps typically provide information over a very limited range of nozzle geometries, which may not apply to the wide range of architectures and designs of aeroengines. The current paper presents a methodology for the development of nozzle performance maps, which takes into account the aerodynamic and the geometric parameters of the nozzle design. The methodology is based on the reduced-order models. These models are integrated into a zero-dimensional engine performance code to improve the accuracy of its thrust calculation. The impact of the new thrust model on the overall engine performance and the operating point is analysed and discussed. The results showed that the implementation of the modified maps, which take into account the flow characteristics and the geometry of the nozzle, affects the thrust calculation. In a typical case of a turbofan operating at cruise conditions, the net thrust estimation with the modified nozzle maps showed a difference of 0.2%, compared with the simple nozzle maps. The new thrust calculation method has the advantage in capturing the multidimensional impact of the flow of the nozzle as compared with the conventional one. Furthermore, the implementation of the new method reduces the uncertainties introduced by a simplified nozzle model and, consequently, it can support the decision-making process in the design of the engine