144 research outputs found

    Stationary rotation of the unbalanced rotor with the liquid autobalancing device under action of external friction forces

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    Influence of external friction forces on rotation of the rotor with the liquid autobalancing device is considered. The liquid in the balancing chamber at stationary movement rotates together with the rotor as solids. Analytical expressions for deflection of the shaft, unbalance of the system and the necessary rotating moment from the engine providing rotation with set speed are received

    Stationary rotation stability of unbalanced rotor with autobalancing device with liquid on flexible shaft

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    The condition of rotor rotation stability with liquid autobalancing device consisting of chamber, float and incompressible homogeneous liquid filling the space between them has been obtained. The restoring force and forces of internal and external friction take effect on the rotor. The latter depend linearly respectively on strain rate and absolute velocity of rotor connection point to the shaf

    Investigation of Input Signal Curve Effect on Formed Pulse of Hydraulic-Powered Pulse Machine

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    Well drilling machines should have as high efficiency factor as it is possible. This work proposes factors that are affected by change of input signal pulse curve. A series of runs are conducted on mathematical model of hydraulic-powered pulse machine. From this experiment, interrelations between input pulse curve and construction parameters are found. Results of conducted experiment are obtained with the help of the mathematical model, which is created in Simulink Matlab

    Optimization of hole generation in Ti/CFRP stacks

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    The article aims to describe methods for improving the surface quality and hole accuracy in Ti/CFRP stacks by optimizing cutting methods and drill geometry. The research is based on the fundamentals of machine building, theory of probability, mathematical statistics, and experiment planning and manufacturing process optimization theories. Statistical processing of experiment data was carried out by means of Statistica 6 and Microsoft Excel 2010. Surface geometry in Ti stacks was analyzed using a Taylor Hobson Form Talysurf i200 Series Profilometer, and in CFRP stacks - using a Bruker ContourGT-K1 Optical Microscope. Hole shapes and sizes were analyzed using a Carl Zeiss CONTURA G2 Measuring machine, temperatures in cutting zones were recorded with a FLIR SC7000 Series Infrared Camera. Models of multivariate analysis of variance were developed. They show effects of drilling modes on surface quality and accuracy of holes in Ti/CFRP stacks. The task of multicriteria drilling process optimization was solved. Optimal cutting technologies which improve performance were developed. Methods for assessing thermal tool and material expansion effects on the accuracy of holes in Ti/CFRP/Ti stacks were developed

    Optical and photoelectron spectroscopy studies of KPb2Cl 5 and RbPb2Cl5 laser crystals

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    The paper presents the results of experimental study of electronic structure of RbPb2Cl5 and KPb2Cl5 laser crystals performed by the optical and photoelectron spectroscopy methods. On the basis of the optical absorption and low-temperature reflection spectra of these crystals we have determined the energy positions of the edges of the low-energy tail of the host absorption, the positions of the first excitonic absorption peaks, and exciton binding energies. The bandgap widths of these crystals at 8 K were estimated as Eg = 4.83 and 4.79 eV, respectively. Qualitative and quantitative analysis of RbPb2Cl 5 and KPb2Cl5 crystals were made on the basis of the core states photoelectron spectra. The elemental composition of the (0 0 1) surfaces of the crystals, the chemical state of the host atoms, the electronic structure of the valence band of the crystals were discussed on the basis on the spectroscopic data. Β© 2012 Elsevier B.V. All rights reserved

    Verification of zinc role in pathophysiology of chronic obstructive pulmonary disease

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    Aim. Determination of the level of zinc and its fractions, as well as the enzyme neutrophilic elastase and albumin in persons suffering from chronic obstructive pulmonary disease (COPD), as well as smoking actively and passively. Materials and methods. The study involved 30 patients with a diagnosis of COPD and 90 healthy persons (60 of them smoking at the present time, 30 - no) who underwent spirometry and determination of zinc levels and its pools, albumin, and neutrophil elastase. All data are subject to statistical processing. Results. It is determined that the studied parameters differ significantly in the groups of smokers with COPD, healthy smokers and non-smokers, and correlate with the volume of forced exhalation for 1 second as a percentage of the due. Conclusion. The revealed regularities make it possible to consider the indicator "share of bound zinc fraction" introduced in the study as a screening criterion in diagnosing COPD in smokers

    Conformation of surface exposed N-terminus part of bacteriorhodopsin studied by transferred NOE technique

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    AbstractInteraction of the monoclonal antibody A5 raised against native bacteriorhodopsin (BR) with the synthetic peptide pGlu1-Ala-Gln-Ile-Thr-Gly-Arg7-NH2, corresponding to the amino acid sequence 1–7 was studied by transferred nuclear Overhauser effect (TRNOE) spectroscopy. The denaturing reagents and the specially designed pulse sequences which eliminate broad signals from the TRNOE spectra were used to favour evaluation of the TRNOE peaks. On the basis of the data obtained, the conformation of peptide bound with A5 was calculated. A model of the mutual arrangement of bacteriorhodopsin N-terminus and the first transmembrane Ξ±-helical segment 8–32 was proposed

    Relationship Between Indices of Oxidative Stress, Endothelial Dysfunction and Chaperone Activity and the Severity of Coronary Atherosclerosis

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    The aim of this research was to study the relationship between the indices of oxidative stress, endothelial dysfunction and chaperone activity of proteins with the severity of coronary atherosclerosis. In patients with coronary heart disease, we found gender-related differences in the severity of coronary atherosclerosis. Significant differences in the indices of oxidative stress, endothelial dysfunction and chaperone activity were revealed depending on the severity of coronary atherosclerosis and the type of atherosclerotic lesion. The determination of studied parameters can serve as a good indicator of the severity of coronary atherosclerosis

    ДСйствиС противовирусных миРНК Π½Π° Π²Ρ‹Ρ€Π°Π±ΠΎΡ‚ΠΊΡƒ Ρ†ΠΈΡ‚ΠΎΠΊΠΈΠ½ΠΎΠ² in vitro

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    Objectives. To evaluate the dynamics of the expression level of IL-1Ξ² and IL-28Ξ² (IFN-Ξ»3) genes as a result of complex knockdown of some cellular genes, whose expression products play an important role in the reproduction of the influenza virus.Methods. Following the collection of virus-containing liquid and cell lysate within three days from the moment of transfection and infection, the intensity of viral reproduction was assessed using the cytopathic effect titration method. The concentration of viral ribonucleic acid (vRNA) and change in the expression of IL-1Ξ² and IL-28Ξ² (IFN-Ξ»3) were determined by real-time reverse transcription quantitative polymerase chain reaction (real-time RT-qPCR). The nonparametric Mann–Whitney test was used to statistically calculate significant differences between groups.Results. The use of each small interfering ribonucleic acid (siRNA) complex led to a decrease in viral reproduction on the first day at the multiplicity of infection (MOI) of 0.001. The use of complex A (FLT4.2 + Nup98.1) and D (FLT4.2 + Nup98.1 + Nup205) led to a decrease in viral titer by 2.8 lgTCID50/mL and by 2.1 lgTCID50/mL relative to the use of nonspecific L2 siRNA and viral control (p ≀ 0.05). Transfection of complexes B (Nup98.1 + Nup205) and C (FLT4.2 + Nup205) also reduced the viral titer by 1.5 lgTCID50/mL and 1.8 lgTCID50/mL relative to nonspecific L2 siRNA and viral control (p ≀ 0.05). When conducting real-time RT-qPCR, a significant decrease in the concentration of viral RNA was also noted. When using complexes B, C, and D, the concentration of vRNA decreased on the first day by 14.5, 4.1, and 15 times, respectively. On the second day, a decrease in vRNA was observed in cells with B and D complexes by 17.1 and 18.3 times (p ≀ 0.05). Along with a decrease in the viral titer and vRNA, an increase in the expression of the IL-1Ξ² and IL-28Ξ² genes was observed on the first day when using all siRNA complexes relative to nonspecific and viral controls (p ≀ 0.05). On the second day, an increase was also observed in cells with A and D complexes, while on the third day, there was an increase in the expression of these genes in cells with complex D (p ≀ 0.05).Conclusions. The use of siRNA complexes is shown to have a pronounced antiviral effect while simultaneously suppressing the activity of cellular genes (FLT4, Nup98 and Nup205). In parallel, the transfection of complexes that block the formation of expression products necessary for viral reproduction is demonstrated to lead to an increase in the level of expression of the IL-1Ξ² and IL-28Ξ² genes. These results indicate not only that the use of siRNA has antiviral activity, but also immunomodulatory activity, which can contribute to a more effective immune response of the body.Π¦Π΅Π»ΠΈ. ΠžΡ†Π΅Π½ΠΈΡ‚ΡŒ Π΄ΠΈΠ½Π°ΠΌΠΈΠΊΡƒ уровня экспрСссии Π³Π΅Π½ΠΎΠ² IL-1Ξ² ΠΈ IL-28Ξ² (IFN-Ξ»3) Π² Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ комплСксного Π½ΠΎΠΊΠ΄Π°ΡƒΠ½Π° Π½Π΅ΠΊΠΎΡ‚ΠΎΡ€Ρ‹Ρ… ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… Π³Π΅Π½ΠΎΠ², Ρ‡ΡŒΠΈ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚Ρ‹ экспрСссии ΠΈΠ³Ρ€Π°ΡŽΡ‚ Π²Π°ΠΆΠ½ΡƒΡŽ Ρ€ΠΎΠ»ΡŒ Π² Ρ€Π΅ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ вируса Π³Ρ€ΠΈΠΏΠΏΠ°.ΠœΠ΅Ρ‚ΠΎΠ΄Ρ‹. Π’ΠΈΡ€ΡƒΡΡΠΎΠ΄Π΅Ρ€ΠΆΠ°Ρ‰ΡƒΡŽ ΠΆΠΈΠ΄ΠΊΠΎΡΡ‚ΡŒ ΠΈ ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹ΠΉ Π»ΠΈΠ·Π°Ρ‚ ΠΎΡ‚Π±ΠΈΡ€Π°Π»ΠΈ Π² Ρ‚Π΅Ρ‡Π΅Π½ΠΈΠ΅ 3-Ρ… Π΄Π½Π΅ΠΉ с ΠΌΠΎΠΌΠ΅Π½Ρ‚Π° трансфСкции ΠΈ зараТСния ΠΈ ΠΎΡ†Π΅Π½ΠΈΠ²Π°Π»ΠΈ ΠΈΠ½Ρ‚Π΅Π½ΡΠΈΠ²Π½ΠΎΡΡ‚ΡŒ вирусной Ρ€Π΅ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ ΠΌΠ΅Ρ‚ΠΎΠ΄Π°ΠΌΠΈ титрования ΠΏΠΎ цитопатичСскому Π΄Π΅ΠΉΡΡ‚Π²ΠΈΡŽ. ΠšΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΡŽ вирусной Ρ€ΠΈΠ±ΠΎΠ½ΡƒΠΊΠ»Π΅ΠΈΠ½ΠΎΠ²ΠΎΠΉ кислоты (вРНК) ΠΈ ΠΈΠ·ΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ экспрСссии IL-1Ξ² ΠΈ IL-28Ξ² (IFN-Ξ»3) опрСдСляли ΠΌΠ΅Ρ‚ΠΎΠ΄ΠΎΠΌ ΠΎΠ±Ρ€Π°Ρ‚Π½ΠΎΠΉ транскрипции ΠΈ ΠΏΠΎΠ»ΠΈΠΌΠ΅Ρ€Π°Π·Π½ΠΎΠΉ Ρ†Π΅ΠΏΠ½ΠΎΠΉ Ρ€Π΅Π°ΠΊΡ†ΠΈΠΈ Π² Ρ€Π΅ΠΆΠΈΠΌΠ΅ Ρ€Π΅Π°Π»ΡŒΠ½ΠΎΠ³ΠΎ Π²Ρ€Π΅ΠΌΠ΅Π½ΠΈ (ОВ-ПЦР-Π Π’). Для вычислСния статистичСски Π·Π½Π°Ρ‡ΠΈΠΌΡ‹Ρ… Ρ€Π°Π·Π»ΠΈΡ‡ΠΈΠΉ ΠΌΠ΅ΠΆΠ΄Ρƒ Π³Ρ€ΡƒΠΏΠΏΠ°ΠΌΠΈ использовали нСпарамСтричСский ΠΊΡ€ΠΈΡ‚Π΅Ρ€ΠΈΠΉ Манна-Π£ΠΈΡ‚Π½ΠΈ.Π Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹. ИспользованиС ΠΊΠ°ΠΆΠ΄ΠΎΠ³ΠΎ комплСкса ΠΌΠ°Π»Ρ‹Ρ… ΠΈΠ½Ρ‚Π΅Ρ€Ρ„Π΅Ρ€ΠΈΡ€ΡƒΡŽΡ‰ΠΈΡ… РНК (миРНК) ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ сниТСнию вирусной Ρ€Π΅ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ Π½Π° 1-Π΅ сутки ΠΏΡ€ΠΈ мноТСствСнности зараТСния 0.001. ΠŸΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ комплСксов A (FLT4.2 + Nup98.1) ΠΈ D (FLT4.2 + Nup98.1 + Nup205) ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΠ»ΠΎ ΠΊ сниТСнию вирусного Ρ‚ΠΈΡ‚Ρ€Π° Π½Π° 2.8 lgΠ’Π¦Π”50/ΠΌΠ» ΠΈ Π½Π° 2.1 lgΠ’Π¦Π”50/ΠΌΠ» ΠΎΡ‚Π½ΠΎΡΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎ примСнСния нСспСцифичСской миРНК L2 ΠΈ вирусного контроля (Ρ€ ≀ 0.05). Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Π΅ трансфСкции комплСксов B (Nup98.1 + Nup205) ΠΈ C (FLT4.2 + Nup205) вирусный Ρ‚ΠΈΡ‚Ρ€ Ρ‚Π°ΠΊΠΆΠ΅ сниТался Π½Π° 1.5 lgΠ’Π¦Π”50/ΠΌΠ» ΠΈ 1.8 lgΠ’Π¦Π”50/ΠΌΠ» соотвСтствСнно ΠΎΡ‚Π½ΠΎΡΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎ нСспСцифичСской миРНК L2 ΠΈ вирусного контроля (Ρ€ ≀ 0.05). ΠŸΡ€ΠΈ ΠΏΡ€ΠΎΠ²Π΅Π΄Π΅Π½ΠΈΠΈ ОВ-ПЦР-Π Π’ Ρ‚Π°ΠΊΠΆΠ΅ Π±Ρ‹Π»ΠΎ ΠΎΡ‚ΠΌΠ΅Ρ‡Π΅Π½ΠΎ достовСрноС ΡƒΠΌΠ΅Π½ΡŒΡˆΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΈ вРНК. ΠŸΡ€ΠΈ использовании комплСксов B, C ΠΈ D концСнтрация вРНК сниТалась Π½Π° 1-Π΅ сутки Π² 14.5, 4.1 ΠΈ 15.0 Ρ€Π°Π· соотвСтствСнно. На 2-Π΅ сутки Π² ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… с комплСксами B ΠΈ D наблюдалось ΡƒΠΌΠ΅Π½ΡŒΡˆΠ΅Π½ΠΈΠ΅ ΠΊΠΎΠ½Ρ†Π΅Π½Ρ‚Ρ€Π°Ρ†ΠΈΠΈ вРНК Π² 17.1 ΠΈ 18.3 Ρ€Π°Π· (Ρ€ ≀ 0.05). Наряду со сниТСниСм вирусного Ρ‚ΠΈΡ‚Ρ€Π° ΠΈ вРНК наблюдалось ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΠ΅ экспрСссии Π³Π΅Π½ΠΎΠ² IL-1Ξ² ΠΈ IL-28Ξ² Π½Π° 1-Π΅ сутки ΠΏΡ€ΠΈ использовании всСх комплСксов миРНК ΠΎΡ‚Π½ΠΎΡΠΈΡ‚Π΅Π»ΡŒΠ½ΠΎ нСспСцифичСского ΠΈ вирусного контроля (Ρ€ ≀ 0.05). На 2-Π΅ сутки Ρ‚Π°ΠΊΠΆΠ΅ наблюдалось ΠΏΠΎΠ²Ρ‹ΡˆΠ΅Π½ΠΈΠ΅ экспрСссии Π² ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… с комплСксами A ΠΈ D, Π° Π½Π° Ρ‚Ρ€Π΅Ρ‚ΡŒΠΈ – Π² ΠΊΠ»Π΅Ρ‚ΠΊΠ°Ρ… с комплСксом D (Ρ€ ≀0.05).Π’Ρ‹Π²ΠΎΠ΄Ρ‹. ИсслСдованиС ΠΏΠΎΠΊΠ°Π·Π°Π»ΠΎ, Ρ‡Ρ‚ΠΎ ΠΏΡ€ΠΈΠΌΠ΅Π½Π΅Π½ΠΈΠ΅ комплСксов миРНК ΠΏΡ€ΠΈΠ²ΠΎΠ΄ΠΈΡ‚ ΠΊ Π²Ρ‹Ρ€Π°ΠΆΠ΅Π½Π½ΠΎΠΌΡƒ противовирусному эффСкту ΠΏΡ€ΠΈ ΠΎΠ΄Π½ΠΎΠ²Ρ€Π΅ΠΌΠ΅Π½Π½ΠΎΠΌ ΠΏΠΎΠ΄Π°Π²Π»Π΅Π½ΠΈΠΈ активности ΠΊΠ»Π΅Ρ‚ΠΎΡ‡Π½Ρ‹Ρ… Π³Π΅Π½ΠΎΠ² (FLT4, Nup98 ΠΈ Nup205). ΠŸΠ°Ρ€Π°Π»Π»Π΅Π»ΡŒΠ½ΠΎ с этим Π±Ρ‹Π»ΠΎ выявлСно, Ρ‡Ρ‚ΠΎ ΠΏΡ€ΠΈ трансфСкции комплСксов, Π±Π»ΠΎΠΊΠΈΡ€ΡƒΡŽΡ‰ΠΈΡ… ΠΎΠ±Ρ€Π°Π·ΠΎΠ²Π°Π½ΠΈΠ΅ ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ‚ΠΎΠ² экспрСссии, Π½Π΅ΠΎΠ±Ρ…ΠΎΠ΄ΠΈΠΌΡ‹Ρ… для вирусной Ρ€Π΅ΠΏΡ€ΠΎΠ΄ΡƒΠΊΡ†ΠΈΠΈ, ΠΏΠΎΠ²Ρ‹ΡˆΠ°Π΅Ρ‚ΡΡ ΡƒΡ€ΠΎΠ²Π΅Π½ΡŒ экспрСссии Π³Π΅Π½ΠΎΠ² IL-1Ξ² ΠΈ IL-28Ξ². Π”Π°Π½Π½Ρ‹Π΅ Ρ€Π΅Π·ΡƒΠ»ΡŒΡ‚Π°Ρ‚Ρ‹ ΡΠ²ΠΈΠ΄Π΅Ρ‚Π΅Π»ΡŒΡΡ‚Π²ΡƒΡŽΡ‚ ΠΎ Ρ‚ΠΎΠΌ, Ρ‡Ρ‚ΠΎ ΠΈΡΠΏΠΎΠ»ΡŒΠ·ΡƒΠ΅ΠΌΡ‹Π΅ миРНК ΠΎΠ±Π»Π°Π΄Π°ΡŽΡ‚ Π½Π΅ Ρ‚ΠΎΠ»ΡŒΠΊΠΎ противовирусной, Π½ΠΎ Ρ‚Π°ΠΊΠΆΠ΅ ΠΈ ΠΈΠΌΠΌΡƒΠ½ΠΎΠΌΠΎΠ΄ΡƒΠ»ΠΈΡ€ΡƒΡŽΡ‰Π΅ΠΉ Π°ΠΊΡ‚ΠΈΠ²Π½ΠΎΡΡ‚ΡŒΡŽ, Ρ‡Ρ‚ΠΎ способствуСт Π±ΠΎΠ»Π΅Π΅ эффСктивному ΠΈΠΌΠΌΡƒΠ½Π½ΠΎΠΌΡƒ ΠΎΡ‚Π²Π΅Ρ‚Ρƒ ΠΎΡ€Π³Π°Π½ΠΈΠ·ΠΌΠ°

    Phase IIb randomized trial of adjunct immunotherapy in patients with first-diagnosed tuberculosis, relapsed and multi-drug-resistant (MDR) TB

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    Placebo-controlled, randomized, phase 2b trial was conducted in 34 adults comprising 18 first-diagnosed (52.9%), 6 relapsed (17.6%), and 10 MDR-TB (29.4%) cases to investigate the safety and efficacy of an oral immune adjunct (V5). The immunotherapy (N = 24) and placebo (N = 10) arms received once-daily tablet of V5 or placebo for one month in addition to conventional anti-TB therapy (ATT) administered under directly observed therapy (DOT)
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