Orthopox and parapox virus specific PCR assay results.

<p>Representative agarose gel electrophoresis of products from PCR assays to detect orthopox or parapox viral DNA. Assays used 50 ng of template from one representative skin sample and appropriate controls. Tracks: M - 1 kbp ladder; 1, 5, 9 - no template control; 2, 6, 10 - sample 41; 3, 7, 11 - vaccinia virus; 4, 8, 12 - sealpox virus. PCR products from the OPV-HA assay (tracks 1–4, predicted size 1138 bp), the PPV-DNApol assay (tracks 5–8, predicted size 536 bp) and the PPV-up/do assay (tracks 9–12, predicted size 84 bp).</p

Transmission electron micrograph of a cetacean skin lesion.

<p>A transmission electron micrograph of sample 4 (<i>Stenella coeruleoalba</i> 2000/4) skin lesion. The box in micrograph <b>A</b>) has been shown at higher magnification in <b>B</b>). Magnification a) 12000×, bar = 1 um and b) 50 000×, bar = 0.4 um.</p

PCR results of cetacean skin samples.

<p>Species: <i>P. phocoena -</i> harbour porpoise (<i>Phocoena phocoena</i>), <i>S. coeruleoalba</i> - striped dolphin (<i>Stenella coeruleoalba</i>), <i>D. delphis</i> – short-beaked common dolphin (<i>Delphinus delphis</i>); Sex: F - Female, M - Male; Age: A - Adult, SA - Sub-adult, J – Juvenile, estimated by length; (T) – tattoo lesion; (? T)- dubious tattoo lesion; (old T) - old tattoo lesion; PPV – parapoxvirus; OPV – orthopoxvirus;</p>*<p>10 ng DNA used as template;</p>#<p>50 ng DNA used as template;</p>§<p>number of+indicates relative amount of PCR product; ND - not done; NA – not applicable.</p

DNA polymerase and DNA topoisomerase I PCR assay results.

<p>Representative agarose gels of PCR products from reactions using 50 ng DNA. <b>A</b>) DNA polymerase assay (predicted product size 543 bp); <b>B</b>) DNA topoisomerase I assay (predicted product size 344 bp). M - 100 bp ladder; track numbers are for sample numbers; 1–25 skin samples; 26 vaccinia virus; 27 sealpox virus; 28 no template control.</p